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Tripeptidyl Peptidase II Plays a Role in the Radiation Response of Selected Primary Cell Types but not Based on Nuclear Translocation and p53 Stabilization

Clinic for Radiotherapy, University Hospital Freiburg, Freiburg, Germany

Requests for reprints: Gabriele Niedermann, Clinic for Radiotherapy, University Hospital Freiburg, 79106 Freiburg, Germany. Phone: 49-761-270-9514; Fax: 49-761-270-9513; E-mail: gabriele.niedermann{at}uniklinik-freiburg.de.

Key Words: -irradiation • tripeptidyl peptidase II • knockout mice • DNA damage response • p53

The giant cytosolic protease tripeptidyl peptidase II (TPPII) was recently proposed to play a role in the DNA damage response. Shown were nuclear translocation of TPPII after -irradiation, lack of radiation-induced p53 stabilization in TPPII-siRNA–treated cells, and complete tumor regression in mice after -irradiation when combined with TPPII-siRNA silencing or a protease inhibitor reported to inhibit TPPII. This suggested that TPPII could be a novel target for tumor radiosensitization and prompted us to study radiation responses using TPPII-knockout mice. Neither the sensitivity to total body irradiation nor the radiosensitivity of resting lymphoid cells, which both strongly depend on p53, was altered in the absence of TPPII. Functional integrity of p53 in TPPII-knockout cells is further shown by a proper G₁ arrest and by the accumulation of p53 and its transcriptional targets, p21, Bax, and Fas, on -irradiation. Furthermore, we could not confirm radiation-induced nuclear translocation of TPPII. Nevertheless, after -irradiation, we found slightly increased mitotic catastrophe of TPPII-deficient primary fibroblasts and increased apoptosis of TPPII-deficient activated CD8⁺ T cells. The latter was accompanied by delayed resolution of the DNA double-strand break marker H2AX. This could, however, be due to increased apoptotic DNA damage rather than reduced DNA damage repair. Our data do not confirm a role for TPPII in the DNA damage response based on nuclear TPPII translocation and p53 stabilization but nevertheless do show increased radiation-induced cell death of selected nontransformed cell types in the absence of the TPPII protease. [Cancer Res 2009;69(8):3325–31]