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Dehydrogenase Studies of Tissue from Normal and Tumor-bearing Mice

I. Total Dehydrogenase Activity

(From the College of St. Scholastica, Unit of the Institutum Divi Thomae, Duluth 2, Minnesota)

Quantitative determinations of the total dehydrogenase activities of the muscle, liver, kidney, brain, and spleen of normal homozygous dba and C57 mice and heterozygous Rockland albino mice revealed a remarkable similarity of activity among the various animal strains. The presence of a tumor (transplanted dbrB adenocarcinoma, methylcholanthrene-induced tumors in dba mice, and transplanted sarcoma 180 in Rockland mice) did not alter the ability of any of the tissue extracts studied to decolorize methylene blue. The total dehydrogenase activity was independent of the sex, strain, and age of the animals, both normal and tumor-bearing. The addition of 0.5 mg. of coenzyme I markedly accelerated the total dehydrogenase activity of the muscle, liver, and brain of normal and tumor-bearing animals, the degree of acceleration in tissues from tumor-bearing animals being about the same as that in tissues from normal animals. The addition of the same amount of coenzyme I to extracts of the different tumors resulted in a pronounced stimulation of activity. The total dehydrogenase activity of the spleen from normal and tumor-bearing animals was not accelerated to any appreciable extent by the addition of a large amount of coenzyme I.