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Antigenic Properties of Nuclei Segregated from Spleens of Normal and Leukemic Mice^*

Kristen Arnesen, Yvette Goldsmith and Anna Dean Dulaney

(From the Sloan-Kettering Institute for Cancer Research, New York 21, New York)

Nuclei have been isolated from spleens of normal and leukemic mice by use of a modified Dounce procedure. These nuclei are relatively pure as judged by morphologic and staining criteria. Such nuclei preparations are poor antigens in that very low titers of antibodies to nuclei are produced upon immunization of rabbits. Antigenic specificity was not demonstrated through use of the complement fixation test. Cross reactions between the nuclei and cytoplasmic fractions were demonstrated in complement fixation tests. Cytoplasmic fractions from the same spleen cells were found to be superior antigens.

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