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Studies with Radioactive Carbon-Labeled Acetate on Cholesterol Metabolism in Rats Fed p-Dimethylaminoazobenzene^*

Carl G. Baker, M.D. and David M. Greenberg, Ph.D.

(From the Division of Biochemistry, University of California, Berkeley 4, California)

1. Radioactive carboxyl-labeled acetate was injected into normal rats and into rats bearing butter yellow-induced hepatomata and the labeled cholesterol was isolated from various individual whole organs by the digitonide method.

2. No significant differences were found in the specific activities of the liver cholesterol in the two groups.

3. A higher percentage of cholesterol was found in the livers of the tumor-bearing animals than in those of the control rats.

4. The ability of the adrenal tissue per unit weight to make cholesterol is much greater than that of any other tissue studied.

5. The coprosterol and dihydrocholesterol digitonides isolated from feces were also radioactive. A discussion of the probable metabolic pathway is presented.

6. The occurrence of a lung tumor in one of the rats which received the butter yellow diet and its corresponding cholesterol study is reported.