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Studies on the Intracellular Composition of Liver and Liver Tumor from Rats Fed 4-Dimethylaminoazobenzene^*

J. M. Price , M.S., J. A. Miller , Ph.D., E. C. Miller, Ph.D. and G. M. Weber, B.S.

(From the MeArdle Memorial Laboratory, Medical School, University of Wisconsin, Madison 6, Wisconsin)

1. The livers and liver tumors of rats fed 4-dimethylaminoazobenzene for 4 to 5 months werehomogenized and separated by differential centrifugation into the nuclei, large granules, smallgranules, and a supernatant fluid (particles non sedimentable at 19,000 x g). The homogenates and fractions were analyzed for protein, nucleic acids, riboflavin, and protein-bound aminoazo dyes.
   
2. Although the total protein contents of the livers and tumors were similar, the nuclear fraction of the tumors contained 52 per cent more, and the large granules 63 per cent less, protein than did the corresponding fractions of the livers.
   
3. Some desoxypentosenucleic acid was found in the large-granule fraction of the livers and in all the cytoplasmic fractions of the tumors. Histological evidence is presented that this unusual dis tribution of desoxypentosenucleic acid was largely due to fragments of the nuclei of white blood cells and necrotic tumor cells.
   
4. The nuclear and supernatant, fluid fractions of the tumors contained almost twice as muchpentosenucleic acid, and the large-granule fraction only half as much, as did the same fractions of the livers.
   
5. The large-granule fraction of the tumors contained only half as much riboflavin as did the same fraction of the livers, but the concentration of the vitamin per gram of protein increased by 38 percent.
   
6. No protein-bound dye was detected in any of the tumor fractions. The percentage distribution of bound dye in the various fractions of the livers was constant from 1 week to 4 months.
   
7. While the composition of each fraction of the liver was altered by the ingestion of the dye, thefractions of the tumors collected either during or after feeding of the dye were nearly the same incomposition.
   

^* This work was supported by grants from the National Cancer Institute, United States Public Health Service, and the Jane Coffin Childs Memorial Fund for Medical Research.

Predoctorate Research Fellow, National Cancer Institute.

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