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Effects of Phosphate Bond Energy on Nucleic Acid Stability in Tumor Homogenates^*

(From the McArdle Memorial Laboratory, Medical School, University of Wisconsin, Madison 6, Wisconsin)

Studies of the nucleic acid phosphorus and ribose in homogenates incubated at 37.6° C. demonstrated that when homogenates of Flexner-Jobling rat carcinoma were provided with ATP by means of an active anaerobic glycolysis, the nucleic acids were maintained. In the absence of a system to maintain ATP, the nucleic acid declined at a linear rate of approximately 23 per cent per hour. Brain homogenates maintained their nucleic acid even in the absence of ATP. Homogenates of liver and kidney gave intermediate rates of decomposition for nucleic acid (9 and 18 per cent respectively).

^* This work was supported by a grant from the American Cancer Society on the recommendation of the Committee on Growth of the National Research Council.