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Carcinogenesis |
Wageningen Centre for Food Sciences, Netherlands Institute for Dairy Research Food Research, Section Nutrition and Health, 6710 BA Ede, the Netherlands [A. L. A. S., D. S. M. L. T., R. V. d. M.], and Department of Gastroenterology, University Hospital, Groningen, the Netherlands [J. H. K.]
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ABSTRACT |
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 Top ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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The colonic epithelial proliferation in heme-fed rats was significantly increased compared to control rats [55.2 ± 5.8 versus 32.6 ± 6.3 dpm/µg DNA (mean ± SE); P < 0.05]. The fecal water of the heme group was highly cytotoxic compared to the controls (90 ± 2% versus 2 ± 1%; P < 0.001), although the concentrations of cytotoxic bile acids and fatty acids were significantly lower. Organic iron was significantly increased compared to the controls (257 ± 26 versus 80 ± 21 µM; P < 0.001). Spectrophotometric analyses suggest that this organic iron is heme-associated. Thiobarbituric acid-reactive substances were greatly increased in the fecal water of heme-fed rats compared to the controls (177 ± 12 versus 59 ± 7 µM; P < 0.05). Heme itself could not account for the increased cytotoxicity because the addition of heme to the fecal water of the control group, which was equimolar to the organic iron content of the fecal water of the heme group, did not influence the cytotoxicity. Hence, an additional heme-induced cytotoxic factor is involved, which may be modulated by the generation of luminal-reactive oxygen species. Protoporphyrin IX, ferric citrate, and bilirubin did not increase proliferation and cytotoxicity. In conclusion, dietary heme leads to the formation of an unknown, highly cytotoxic factor in the colonic lumen. This suggests that, in heme-fed rats, colonic mucosa is damaged by the intestinal contents. This results in a compensatory hyperproliferation of the epithelium, which supposedly increases the risk for colon cancer.
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INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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To explain this latter association, several hypotheses have been proposed, which were summarized recently (7) . First, risk for colon cancer is epidemiologically linked to the consumption of well-done fried meat, which is probably due to the presence of heterocyclic aromatic amines. These compounds are true carcinogens in animal models. However, the contribution of heterocyclic amines to human colon cancer incidence is thought to be very low because doses required for carcinogenicity in animal studies exceed the daily human intake by several orders of magnitude. Furthermore, the hypothesis is challenged by the fact that levels of heterocyclic amines in cooked white meat exceed levels in red meat (8) . Therefore, heterocyclic amines cannot explain the differential effects of red and white meat. Secondly, in the gastrointestinal tract, the reaction of nitrosating agents like NO and N2O3 with amines can form N-nitroso compounds. Although proven to be mutagenic in vitro, the carcinogenicity of these compounds in humans is still under debate. According to the third hypothesis, consumption of meat increases the intake of fat, which itself is often regarded as a risk factor for colon cancer. Dietary fat is thought to act, at least in part, by increasing the intracolonic concentrations of membrane-damaging bile acids and fatty acids or via the production of the potentially mitogenic diacylglycerol. Large prospective cohort studies, however, have shown that the association of colon cancer and red meat cannot be explained solely by the fat content of the meat (9) . The fourth hypothesis suggests a role for dietary iron in colorectal carcinogenesis because of its catalytic activity in the formation of oxygen radicals (10 , 11) . However, in animal studies in which different forms of iron were tested, a clear role for iron in colon cancer could not be established (12 , 13 , 14) .
It should be noted that, although iron from meat is mainly in the form of heme (content in beef is 
1.5 µmol heme/g dry weight; Ref. 15
), specific effects of the heme molecule on the colonic epithelium have not hitherto been considered. Heme absorption is very low, and most ingested heme is therefore delivered to the colon. It was shown that after consumption of red meat but not of chicken or fish (which have a low heme content), heme could be recovered from the feces (15)
. Heme is an amphipathic molecule with a bulky, hydrophobic tetrapyrrole ring structure with two propionic acid side chains. Earlier studies in our laboratory have shown that other amphipathic molecules, such as bile acids and fatty acids, can cause epithelial damage, resulting in a compensatory epithelial hyperproliferation (16)
. Furthermore, it was shown that heme is cytotoxic toward mammalian cells in vitro (17)
. Therefore, we hypothesized that dietary heme or its metabolic degradation products causes cytotoxic effects in the colonic lumen, which may affect the proliferation of the colonic epithelium. We tested this in rats and compared heme with equimolar amounts of other tetrapyrroles, protoporphyrin IX and bilirubin, which are normal physiological heme metabolites (15
, 18)
. Furthermore, because heme is an important iron carrier, ferric citrate was also included in the study.
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MATERIALS AND METHODS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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In Vivo Colonic Proliferation.
After the experimental feeding period of 14 days, nonfasted rats received injections i.p. of [methyl-[3H]thymidine (specific activity, 25 Ci/mmol; dose, 100 µCi/kg body weight; Amersham International, Buckinghamshire, United Kingdom) in 154 mM NaCl. After 2 h, they were killed by CO2 inhalation, and the colon was removed and longitudinally opened. Colonic contents were removed by rinsing with 154 mM KCl, and the mucosa was scraped off with a spatula and homogenized in 1 ml 154 mM KCl (Ultraturrax Pro200: Pro Scientific Inc., Monroe, CT). The scrapings were analyzed as described previously (20)
. For protein determinations, 100 µl of homogenate were diluted 10-fold with double-distilled water, and deoxycholic acid was added (final concentration, 0.15 mg/ml). After incubation for 10 min at room temperature, protein was precipitated with trichloroacetic acid (final concentration, 60 mg/ml). Samples were centrifuged for 15 min at 3,000 g, and the pellet was resolubilized in SDS (50 mg/ml) in 100 mM NaOH. Protein was quantified according to Smith et al. (21)
using the 2 Bicinchoninic Acid Protein Assay Kit (Pierce, Rockford, IL), with BSA as the standard.
Fecal Water Preparation.
Fecal water was prepared by reconstituting a small amount of freeze-dried feces with double-distilled water to 30% dry weight. After homogenizing, samples were incubated for 1 h in a shaking water bath at 37°C, with mixing every 15 min, followed by centrifugation for 10 min at 15,000 g. Then, supernatants were centrifuged at 15,000 g for another 2 min. The supernatant was aspirated, and osmolarity was measured (Osmomat 030-D, Gonotec, Berlin, Germany). If osmolarity differed from 300 mOsmol/L, another portion of freeze dried feces was reconstituted with double-distilled water, and the percentage dry weight was adjusted to obtain fecal water with an osmolarity of 300 mOsmol/L. Fecal water was stored at -20°C until analysis.
Cytotoxic Activity Assay.
Cytotoxic activity of fecal water was quantified by potassium-release from erythrocytes as described by Govers et al. (22)
with the following modifications. The dose-dependent cytotoxicity of pooled fecal water of control and heme groups was determined by supplementing increasing volumes of fecal water with saline to a total volume of 80 µl. After preincubation for 5 min at 37°C, 20 µl of a washed human erythrocyte suspension were added (final hematocrit, 5%) and incubated for 15 min at 37°C. Cytotoxicity was measured as described before (22)
. Subsequently, differences between experimental groups were quantified by testing the cytotoxicity of 10 µl of fecal water. Finally, to test whether fecal cytotoxicity of heme-fed rats was dependent on oxygen, the cytotoxicity of increasing volumes of fecal water of the heme group was determined as described above in an anaerobic cabinet (Coy Laboratory products, Ann Arbor, MI). Simultaneously, equal amounts were tested at ambient atmosphere.
Total Feces Analyses.
For total iron determination, feces were dry-ashed for 8 h at 550°C (Heraeus, Eurotherm 815, Boom Meppel, the Netherlands), followed by destruction (20 min; 180°C) with 500 µl of perchloric acid (70%) and 100 µl of H2O2 (30%). After dilution in double-distilled water, iron was measured using an atomic absorption spectrometer (Perkin-Elmer, model 1100, Norwalk, CT). The recovery of added ferric citrate was 95% ± 7%, and that of hemin was 85% ± 5%.
To measure sodium and potassium, feces were treated with 5% trichloroacetic acid for 1 h at room temperature and centrifuged for 2 min at 14,000 g. The supernatants were diluted with 0.05% CsCl, and sodium and potassium were measured by atomic emission spectrometry. For the determination of the ammonia content, feces were incubated for 15 min at 37°C in 5% perchloric acid. Samples were centrifuged for 2 min at 14,000 g, and ammonia was measured in the supernatant by using the Sigma Urea Nitrogen Kit (Sigma Diagnostics, No. 640, St. Louis, MO) and omitting the incubation step with urease. The percentage of water in the feces was calculated assuming that the total amount of sodium, potassium, ammonia, and their negatively charged counterions provided an osmolarity of 300 mOsmol/L in feces (23) .
Analyses of Fecal Water.
To determine the total iron in fecal water, samples were treated as described for feces except that the dry-ashing step was omitted, and 50 µl of perchloric acid and 20 of µl H2O2 were used for destruction. For inorganic iron determination, fecal water was incubated in 10% trichloroacetic acid for 20 min at 90°C and centrifuged at 14,000 g for 2 min. The supernatant was diluted in double-distilled water, and inorganic iron was measured by atomic absorption spectrometry. Organic iron was calculated as the difference between total iron and inorganic iron. Using these procedures, the recovery of added ferric citrate as inorganic iron was 97% ± 2%, and the recovery of hemin as organic iron was 92% ± 7%. For the determination of free fatty acids and bile acids in fecal water, acidified fecal water (final HCl concentration, 1M) was extracted three times with five volumes of diethyl ether. The diethyl ether phase was dried under nitrogen, and the extract was resolubilized in ethanol. Free fatty acids were determined using a colorimetric enzymatic assay (NEFA-C kit, Wako Chemicals, Neuss, Germany), and bile acids were measured with a fluorescent enzymatic assay, as described earlier (20)
.
For spectrophotometric analyses, an acidified chloroform-methanol extract (final HCl concentration, 1M) was obtained (24) . The chloroform phase was dried under nitrogen and resolubilized in methanol. An absorption spectrum was recorded from 300 to 700 nm on a spectrophotometer (Perkin-Elmer, Lambda 2, Norwalk, MO).
To evaluate lipid peroxidative processes in the lumen, TBARSs3 in fecal water were measured according to Ohkawa et al. (25) . Briefly, fecal water was diluted 10-fold with double-distilled water, and 100 µl of this diluted fecal water was mixed with 100 µl of 8.1% SDS and 1000 µl of 0.5% TBA in 10% acetic acid (pH 3.5). For blanks, TBA was omitted from the assay. After heating for 60 min at 95°C, TBARSs were extracted with 1.2 ml n-butyl alcohol. The absorbance of this butanol extract was measured at 532 nm. The amount of TBARSs was calculated as malondialdehyde equivalents by using 1,1,3,3,-tetramethoxypropane as the standard.
Statistics.
Results are presented as means ± SE (n = 8). A commercially available package (SPSS/PC + v2.0, SPSS Inc., Chicago, IL.) was used for all statistics. In the case of normally distributed data, one-way ANOVA was performed, followed by Fisher’s LSD test (two-sided) to test for significant differences between means of dietary treatments and control diet. When data of groups showed unequal variances, the distribution of data was normalized using ln-transformation, and data were treated as described above. If variances were still unequal, the nonparametric Kruskal-Wallis test was used, and differences between means were tested with the Mann-Whitney U test for significance (two-sided). For all parameters, data from each experimental group were compared with control data only.
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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. It shows that in both high-iron groups, fecal iron was significantly increased, whereas it was not affected by protoporphyrin IX or bilirubin. From these data, it can be calculated that apparent iron absorption from the control diet was 12%. Iron supplementation by either heme or ferric citrate did not increase the apparent iron absorption. Thus, a large majority of dietary heme iron and ferric citrate reached the colon. There, only heme iron had striking effects on fecal parameters not shown by the other diets (Table 1)
. Daily fecal output (total dry weight) was higher when heme was added to the diet. During the experiment, the softening of feces of heme-fed rats was observed, whereas feces from other experimental groups had an appearance similar to control feces. These symptoms may reflect a disturbance of the absorption or secretion function of the colon. Therefore, we measured major fecal cations (sodium, potassium, and ammonia) and found that these were greatly increased by dietary heme. Consequently, the net absorption of water was lower, as reflected by a higher fecal wet weight (%) in the heme group.
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shows that the heme diet significantly increased proliferation compared with the control diet, whereas proliferation was not affected by the other dietary treatments. DNA and protein content did not differ among the groups (DNA, 874 ± 83 µg/scraping; protein, 9.1 ± 1.0 mg/scraping).
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shows that the colonic cytotoxicity was extremely high in the heme group: already 10 µl of fecal water were sufficient to lyse all erythrocytes. This implies that at >10 µl, the cytotoxicity of the fecal water of the heme group would be underestimated. Therefore, the cytotoxicity of all fecal waters was tested using 10 µl in our bioassay; the results are shown in Fig. 3
. Again, only the heme group showed increased cytotoxicity of fecal water, whereas all other experimental groups did not differ from the control. Because bile acids and fatty acids are important determinants of fecal water cytotoxicity (16)
, we tested whether their concentrations were increased by dietary heme. This was not the case because fewer bile acids and fatty acids were present in the fecal water of the heme group (Fig. 4)
. Because we showed that about 90% of dietary heme reached the colon, we determined whether heme or heme-derived factors were solubilized in the aqueous phase. Fig. 5
shows that whereas both iron-supplemented groups had slightly elevated levels of total iron, only heme iron increased the organic iron content of the fecal water. In addition, Fig. 6
shows that there was a concomitant large increase in the absorbance at 400 nm of the fecal water for the heme group and a moderate increase for the protoporphyrin group, whereas the spectra of the ferric citrate and the bilirubin groups were not different from the control. Together, these results suggest that heme was solubilized in the aqueous phase of the feces. Because heme has been shown previously to be lytic toward erythrocytes (17)
, we tested whether heme itself could be responsible for the extreme cytotoxicity observed in the feces of the heme-fed rats. However, when we added 250 µM of heme to the fecal water of the control rats, which is equivalent to the organic iron concentration of fecal water of the heme group, the cytotoxicity was not enhanced under our experimental conditions (results not shown).
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. To assure that the TBARSs were not formed artificially during the preparation of the fecal water or during the TBARS assay, heme was added to lyophilized feces of the control group, and fecal water was prepared. No increase in TBARSs was seen in the fecal water of the heme-supplemented control feces compared with the control fecal water. In addition, the reactivity of the control fecal water toward TBA was not affected by the addition of heme directly in the assay. Finally, to test whether oxygen radicals themselves might be responsible for the heme-induced cytotoxicity, we determined the dose-dependent cytotoxicity of the fecal water of the heme group in an anaerobic cabinet and at ambient atmosphere. No significant differences were observed (results not shown). Thus, the cytotoxicity of fecal water did not depend on the presence of oxygen.
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DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Thus, the ingestion of heme obviously leads to the formation of very cytotoxic compounds in the intestinal lumen. Our results may shed some light on the nature of this heme-induced cytotoxic factor. Fig. 4
, which shows that the concentrations of bile acids and fatty acids were lower in fecal water of heme-fed rats compared with controls, suggests that these surfactants also were not responsible for the heme-induced cytotoxicity. This means that in heme-fed rats, an additional cytotoxic factor is formed and solubilized in fecal water. Fig. 5
shows that the total iron content in fecal water was not different between the heme group and the ferric citrate group. This implies that the total iron in fecal water is not related to cytotoxicity and hyperproliferation. However, the organic iron content of fecal water was much higher in the heme group than in the other dietary groups. In addition, a sharp rise in absorbance at 400 nm was observed in the fecal water of the heme group (Fig. 6)
. Heme compounds show a specific absorption at 400 nm (Soretband). These two observations indicate that part of the organic iron in fecal water might be intact heme that has escaped intestinal absorption and metabolic conversion. Indeed, others have shown that part of the dietary heme appears unmetabolized in feces (15
, 29
, 30)
. So the high cytotoxicity in the heme group could simply be caused by the heme itself. However, the addition of equimolar amounts of heme to fecal water of the control group did not enhance the cytotoxicity. Well-known metabolites of heme in the gastrointestinal tract are protoporphyrin, inorganic iron (15
, 29
, 30)
, and bilirubin (18)
. No increase in the cytotoxicity of the fecal water or in epithelial proliferation was seen when these compounds were added to the diet. Thus, a role for these luminal-derived products as a cytotoxic factor can be excluded. Our observation that bilirubin is inert in our model contrasts with the work of Babbs (11)
who suggested that iron, which acts as a catalyst in oxygen radical formation, is solubilized by linear tetrapyrroles like bilirubin. We question this role for linear tetrapyrroles in vivo because our spectrophotometric analyses show that bilirubin (which has an absorption maximum at 453 nm) is not solubilized in fecal water (Fig. 6)
. Hence, it does not chelate soluble iron in vivo. Moreover, we showed that the addition of bilirubin to the diet did not affect any luminal or mucosal parameter.
It is tempting to speculate that the formation of the cytotoxic compound may be due to the action of oxygen radicals. The increase in fecal TBARSs suggests that these reactive oxygen species are indeed formed in the colonic lumen of heme-fed rats. However, these radicals do not seem to be directly involved in heme-induced cytotoxicity because under both aerobic and anaerobic conditions, the cytotoxicity of fecal water was the same. Due to the presence of thirteen unsaturated bonds, heme itself seems particularly vulnerable to attack by these highly reactive oxygen species. Future research is needed to elucidate the nature of the heme-induced cytotoxic factor. This should provide more insight into its formation as well as into the mechanism of its cytotoxic effects.
In this study, heme was used as a model compound for the consumption of red meat. In the literature, only a few experimental studies regarding the effect of beef on tumor development in rats are reported. Rats who received injections of the experimental carcinogen dimethylhydrazine developed more colon tumors when they were beef-fed (31) . On the other hand, Lai et al. (32) observed no effect of lean beef on the formation of dimethylhydrazine-induced colon tumors. These contrasting results suggest that the effect of the carcinogen may be dependent on the dietary background. McIntosh et al. (31) used a high-fat diet (20% fat, w/w), which may promote colon tumor formation induced by carcinogens (33) compared with low-fat diets as used by Lai et al. (Ref. 32 ; 6.25% fat, w/w). This implies that if dimethylhydrazine-induced tumor formation is mediated by heme iron from beef, these heme effects can be modulated by other dietary factors. We think that our heme model is very well-suited to study possible interactions of heme with other dietary ingredients; for instance, the effect of poorly absorbed nutrients like calcium, fiber, or some antioxidants, which are epidemiologically associated with a decrease in colon cancer risk.
In conclusion, dietary heme induces fecal cytotoxicity and hyperproliferation of the colonic mucosa in rats. Consumption of red meat but not of white meat is associated with a high risk for colon cancer. Because the heme content of red meat is 10-fold higher than that of white meat (15) , we suggest that the association between red meat consumption and colon cancer may be due to its high content of heme iron. Because the formation of the highly cytotoxic factor in feces was specific for dietary heme, identification and quantification of this compound in fecal water would provide further insight into the molecular mechanism of the relationship between red meat consumption and colon cancer observed in epidemiological studies.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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1 Supported by Grant 904-62-167 from The Netherlands Organization for Scientific Research, Medical Sciences. An abstract of this study was presented at the Digestive Disease Week 1998. 
2 To whom requests for reprints should be addressed, at Wageningen Centre for Food Sciences, NIZO Food Research, Section Nutrition and Health, P. O. Box 20, 6710 BA Ede, the Netherlands. Phone: 31-318-659621; Fax: 31-318-650400; E-mail: Sesink{at}nizo.nl
3 TBARS, thiobarbituric acid-reactive substance; TBA, thiobarbituric acid; LSD, least significant difference.
Received 4/16/99. Accepted 9/22/99.
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REFERENCES |
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