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Tumor Biology |
Divisions of Experimental Therapy [M. M., M. A. v. G., A. H. S., J. H. M. S.], Medical Oncology [J. H. M. S.], and Pathology [I. F. F., M. J. v. d. V.], The Netherlands Cancer Institute, 1066 CX Amsterdam; and Department of Pathology, Free University Hospital, 1081 HV Amsterdam [G. L. S., A. C. L. M. P., R. J. S.], the Netherlands
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ABSTRACT |
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 Top ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Using BXP-21 and BXP-34, prominent staining of BCRP was observed in placental syncytiotrophoblasts, in the epithelium of the small intestine and colon, in the liver canalicular membrane, and in ducts and lobules of the breast. Furthermore, BCRP was present in veinous and capillary endothelium, but not in arterial endothelium in all of the tissues investigated. In the tissues studied, the mRNA levels of BCRP were assessed using reverse transcription-PCR, and these corresponded with the levels of BCRP protein estimated from immunohistochemical staining. The presence of BCRP at the placental syncytiotrophoblasts is consistent with the hypothesis of a protective role of BCRP for the fetus. The apical localization in the epithelium of the small intestine and colon indicates a possible role of BCRP in the regulation of the uptake of p.o. administered BCRP substrates by back-transport of substrate drugs entering from the gut lumen. Therefore, it may be useful to attempt to modulate the uptake of p.o. delivered BCRP substrates, e.g., topotecan or irinotecan, by using a BCRP inhibitor. Clinical trials testing this hypothesis have been initiated in our institute.
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INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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For many drug transporters, a normal physiological role is known. For instance, P-gp is highly expressed in the blood-brain barrier, the intestine, and the placenta and has a protective function for the brain and the fetus by extruding toxic agents (6) . MRP1 also appears to function as an outward pump for xenobiotics (7) and contributes to the blood-cerebrospinal fluid barrier (8 , 9) . For BCRP, the normal physiological function has not been established as yet. In normal human tissues, high expression of BCRP mRNA has been noted in the placenta (1 , 4) . Furthermore, although results between previous studies (1 , 4) differ somewhat, low expression of BCRP in liver, small intestine, colon, ovary, kidney, and heart was reported. This expression profile allows speculation on a role of BCRP, like P-gp, in protection of the fetus and in the regulation of transport of chemicals through the epithelium of the gastrointestinal tract. The mRNA expression of BCRP has been assessed in tissue extracts, but it has not yet been determined in which cell types BCRP is expressed and what the subcellular localization of BCRP is.
We have developed recently the Mab BXP-34 directed against human BCRP (10) . In this study, we describe a newly developed Mab directed against BCRP, BXP-21. We have used the BXP-21 and BXP-34 Mabs to characterize the tissue distribution and subcellular localization of BCRP. Moreover, for a number of tissues we isolated mRNA from the same blocks for semiquantitative RT-PCR evaluation of the BCRP expression levels. Knowledge of the normal tissue distribution may add to the understanding of the normal function of BCRP and may be valuable for future clinical purposes.
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MATERIALS AND METHODS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Immunization and Mab Production.
The development of the Mab BXP-34, directed against BCRP, has been described elsewhere (10)
. An independently developed Mab, BXP-21, was raised in Balb/C mice analogous to described methods (10
, 18)
by injection with a fusion protein consisting of the Escherichia coli maltose-binding protein and a 126 amino acids part of the BCRP peptide [amino acids 271-396 of BCRP (GenBank accession no. AF098951)]. This fusion protein was made using the plasmid vector pMal-c (19)
. The fusion protein was produced in Escherichia coli DH5
and purified by amylose resin affinity chromatography (19)
. The mice were housed and treated according to current regulations and standards of the Institutional Animal Ethics Committee.
The mice were killed, and subsequently draining popliteal lymph nodes were removed and used for fusion with mouse myeloma Sp2/0 cells, as described previously (14) . Hybridoma supernatants containing Mabs were first screened on ELISA plates, coated with the above-mentioned fusion protein, or coated with an irrelevant fusion protein as negative control. BCRP fusion protein-positive cultures were screened on octo-spins containing eight cytospins of a mixture of MCF-7 MR and MCF-7 parental tumor cells. Slides were stained as described below. Hybrid cells that secreted antibodies of interest were selected and subcloned three times by limiting dilution. The isotype was determined using IsoStrips (Boehringer Mannheim).
Western Blotting.
Cells were scraped and subsequently lysed in hypotonic lysis buffer, consisting of 100 µM KCl, 2 µM MgCl2, 100 µM Tris-HCl (pH 7.4), 1% SDS, supplemented with protease inhibitors ("Complete"; Roche Diagnostics, Germany). Lysates were sonicated and stored at -80°C. Protein levels were determined using the Lowry method. Proteins were separated on a 7.5% polyacrylamide gel and subsequently transferred electrophoretically to nitrocellulose membranes (Schleicher & Schuell, Dassel, Germany). Proteins were hybridized using BXP-21 (1:50) and HRP-conjugated goat antimouse IgG (1:1000; Dako, Glostrup, Denmark). For control purposes, P-gp, MRP1, and MRP2 were hybridized using C219 (20)
, MRPr1 (18)
, and M2III-6 (21)
as primary antibodies, respectively. Subsequently, proteins were visualized using ECL (Amersham Life Sciences, ’s-Hertogenbosch, the Netherlands).
Immunoprecipitation.
Cells were preincubated for 2 h with RPMI without methionine and without FCS before labeling. Cells were labeled overnight with 4 µCi/ml [35S]methionine (Amersham Life Sciences) in RPMI without methionine with 10% FCS. Cells were homogenized in lysis buffer (PBS, 1% NP40, 1 mM EDTA, and "Complete" protease inhibitors) for 30 min on ice. Subsequently, nuclei and large debris were removed by centrifugation for 1 min at 14,000 rpm. Supernatants containing approximately 5 x 105 cpm were diluted with lysis buffer and incubated for 1.5 h at 4°C with 2 µg BXP-34 or BXP-21. Immune complexes were precipitated by the addition of 100 µl of 10% protein A-Sepharose in PBS (CL-4B; Pharmacia, Uppsala, Sweden) and incubation at 4°C for 1 h. Precipitates were washed twice with lysis buffer containing 2% BSA and four times with PBS. Next, samples were taken up in 50 µl of sample buffer [final concentrations, 62.5 mM Tris-HCl (pH 6.8), 10% glycerol, 3% SDS, 5% 2-mercaptoethanol, and 0.05% bromphenol blue]. Protein A-Sepharose was removed by centrifugation, and samples were loaded on a 7.5% polyacrylamide gel. Gels were stained with Coomassie Blue (BluePrint Fast-PAGE Stain; Life Technologies, Inc.) and incubated with NAMP100 amplifier (Amersham Life Sciences). Subsequently, gels were analyzed using a phosphorimaging system (Fujix Bas 2000; Fuji Photo Film Co. Ltd., Tokyo, Japan).
Deglycosylation.
Half of a batch of cell lysates of [35S]methionine-labeled T8 and MX3 tumor cells, as prepared for immunoprecipitation, were incubated with 2 units of peptide-N-glycosidase F (Roche Diagnostics Nederland B.V., Almere, the Netherlands) at 37°C for 1 h, and the other half was mock-treated under identical conditions. Subsequently, samples were treated as described above for the immunoprecipitation, and gels were analyzed using the phosphorimager.
Immunohistochemistry.
Cryostat sections (4 µm) were cut, dried overnight at room temperature, and fixed in acetone for 8 min at room temperature. Cytospin preparations of tumor cells were also fixed in acetone. Formaldehyde-fixed paraffin-embedded tissues were deparaffinized in xylene and rehydrated. Endogenous peroxidase activity was blocked using 0.3% (v/v) H2O2 in methanol for 20 min. Before staining, paraffin sections were pretreated with 10 mM citric acid (pH 6.0) for 20 min. The slides were first incubated with 5% normal goat serum/PBS for 30 min. Subsequently, frozen sections and cytospins were incubated for 60 min at room temperature with a 1:150 or 1:100 dilution of BXP-21 or BXP-34 hybridoma supernatant, respectively, whereas paraffin sections were incubated with a 1:150 or 1:100 dilution of BXP-21 or BXP-34, respectively, at 4°C overnight. BXP-21 and BXP-34 were diluted in PBS/BSA.
Two staining methods were applied. In the first method, biotinylated goat antimouse IgG (Dako; 1:200) and HRP-conjugated streptavidin (both in 90% PBS/BSA + 10% normal human serum) were used as secondary reagents. Color development was performed using 0.4 mg/ml AEC. The second method used the tyramide/FITC amplification method, as described by De Vree et al. (22) . In this case, after incubation with the BXP-34 or BXP-21 Mabs, slides were incubated for 60 min with HRP-conjugated goat antimouse IgG (Dako; 1:100 in 90% PBS/BSA + 10% normal human serum), subsequently incubated for 10 min with 1:100 tyramide/FITC in amplification buffer (NEN Life Science Products, Boston, Massachusetts), and finally incubated for 60 min with HRP-conjugated rabbit anti-FITC (Dako; 1:100 in PBS/BSA). Color development was achieved using AEC. After counterstaining with hematoxylin, slides were mounted.
For each type of tissue, negative controls were included, i.e., by omission of the primary Mab, by using the irrelevant IgG1 Mab MOPC 21 (ICN Pharmaceuticals, Aurora, Ohio) or the IgG2a Mab PI 17 (American Type Culture Collection, Manassas, Virginia).
BCRP Expression in Cell Lines and Normal Human Tissue.
Poly(A)+ RNA was isolated from cell lines or from 30 x 30-µm cryosections/tissue sample using RNAzol, according to the manufacturer’s description. mRNA aliquots (3 µg) were used for semiquantitative RT-PCR. The PBGD (NM000190) gene was used as an internal standard. This housekeeping gene was selected, because it is expressed independently of the cell cycle (23
, 24)
. The following primers were applied: 5'-caaccattgcatcttggctg-3' (forward, nt 1914-1933) and 5'-caaggccacgtgattcttcc-3' (reverse, nt 2118-2137) for BCRP and 5'-tctggtaacggcaatgcggc-3' (forward, nt 31-50) and 5'-ccagggcatgttcaagctcc-3' (reverse, nt 264-283) for PBGD. For each RNA sample, 12 reactions were performed using 14, 16, and 18 to 36 cycles. Water was amplified for a total of 36 cycles as a negative control. DNA was labeled using [-32P]dCTP, and products were separated electrophoretically on a 6% polyacrylamide gel. DNA bands were quantified using a phosphorimaging system. Finally, the relative expression of BCRP as compared with that of PBGD (BCRP/PBGD) was calculated using the method of de Lange et al. (25)
. Briefly, at least four intensities of bands were measured in the log-linear part of the amplification cycle versus signal intensity curves, and subsequently the distance between the curves of the BCRP gene and that of the PBGD gene was used to calculate the relative expression levels. The assay was performed in duplicate. Poly(A)+ RNA from the human ovarian IGROV1 and the human small cell lung cancer cell line GLC4-ADR (both having very low levels of BCRP), the BCRP-overexpressing cell line T8 (high level of BCRP; Ref. 3
), and the partial revertant T8-40 (intermediate levels of BCRP; Ref. 3
) was used as control.
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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. Specificity of the antibody was further demonstrated using BCRP-transfected monkey kidney COS7 cells, as described previously for BXP-34 (10
; data not shown). BXP-34 was shown before to not be suitable for Western blotting of BCRP (10)
. However, by using BXP-21, a band of approximately Mr 72,000, corresponding to the predicted molecular weight of BCRP, was detected in Western blots of BCRP-overexpressing tumor cell lines (Fig. 2)
. Relative levels of BCRP correlated with known mRNA expression levels of BCRP in the cell lines studied (2
, 3) . BXP-21 did not cross-react with the known MDR transporters P-gp, MRP1, and MRP2 in cytospin preparations (data not shown) and in Western blots (Fig. 3)
. Immunoprecipitation experiments using BXP-21 or BXP-34 Mabs resulted in bands of approximately Mr 72,000, whereas no bands were detected at this position using an isotype-matched irrelevant antibody (Fig. 4A)
. Interestingly, the molecular weight of the bands in the T8 and MX3 differed slightly in both Western blots and immunoprecipitation experiments. Treatment with peptide-N-glycosidase F yielded bands with an equal, slightly lower, molecular weight of approximately 62,000 in both cell lines, showing that this difference was caused by different levels of glycosylation in these cell lines (Fig. 4B)
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; Fig. 1
). In several normal human tissues, strong immunoreactivity was observed (Table 2)
. Placental tissue showed apical staining of the syncytiotrophoblasts (Fig. 5A)
, whereas liver was strongly stained at the bile canalicular membrane (Fig. 5B)
. Prominent staining was observed in the gastrointestinal tract, with strong apical staining of the epithelium of the small intestine and colon (Fig. 5, C and D
, respectively), and at the apical side in a proportion (but not all) of the ducts and lobules of the breast (Table 2)
. Furthermore, in almost all of the tissues tested, staining of the venous and capillary endothelial cells was observed (e.g., Fig. 5, E and F
). In contrast, only sporadic staining for BCRP was observed in arterial endothelium (Fig. 5, G and H)
. No significant staining was observed in blood cells, i.e., erythrocytes, leukocytes, and platelets.
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. Using this assay, we confirmed previously reported (1
, 4)
high expression of BCRP in the placenta (BCRP/PBGD ratio of 4.2, as compared with 5.0 in the T8 cell line). In general, results from the RT-PCR assay were consistent with the immunohistochemical staining. Because of blood vessel endothelial expression of BCRP, mRNA levels of BCRP were partly influenced by the density of blood vessels in the respective tissues; e.g., low levels of immunohistochemical staining in the heart and breast correlated with low BCRP/PBGD ratios (0.26 and 0.85, respectively). High levels of BCRP mRNA in the cervix and ovary (ratios, 3.25 and 5.40, respectively) corresponded with extensive immunohistochemical staining of many blood vessels in the tissue sections used in this study (for ovary, see Fig. 5F
). Furthermore, the relatively large epithelial (villous) surface in the small intestine sections corresponded with a higher BCRP/PBGD ratio than in the colon sections (ratios, 3.85 and 1.60, respectively). |
DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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From previously reported mRNA data (1
, 4)
, BCRP is known to be highly expressed in the placenta and at lower levels in the liver, small intestine, colon, and ovary. In one study (4)
, low expression of BCRP was observed in the kidney and heart as well. Using semiquantitative RT-PCR, we confirmed the expression of BCRP in these tissues, although the relative levels differed. These differences may be caused by the sections of the respective tissues that were used in these studies. Notably, in contrast to the study of Allikmets (4)
, the expression level of BCRP in the heart, as determined by our semiquantitative RT-PCR assay, was one of the lowest of all of the tissues tested, in line with our staining results in the heart (Table 2)
. Furthermore, our RT-PCR data showed that low to moderate levels of BCRP mRNA are present in every tissue tested. This background level of BCRP was explained by immunohistochemical staining, which showed BCRP to be present in endothelium of veins and capillaries. The importance of endothelial expression of BCRP for the observed tissue expression levels of BCRP is illustrated by our results in ovary and cervix tissue. These tissues contain many blood vessels and indeed showed very high expression of BCRP for the ovary, even higher than that found in placental tissue (Table 2)
.
Nonendothelial staining for BCRP was observed in only a limited number of tissues, i.e., the placenta, small intestine, colon, liver, and breast. The presence of BCRP in placental syncytiotrophoblastic cells indicates that BCRP may have a protective function for the fetus. Interestingly, both in wild-type and in mdr1a/1b(-/-) mice, inhibition of mouse Bcrp1 by GF120918, a recently described inhibitor of human BCRP and mouse Bcrp1 (27 , 28) , resulted in at least 2-fold increased uptake of p.o. administered BCRP substrate topotecan in the fetus (29) . Notably, relative mRNA levels of mouse Bcrp1 in the placenta are lower than that observed in humans; therefore, the protective effect in humans may well be stronger than that observed in mice.
Furthermore, prominent apical staining of BCRP was observed in the epithelium of both the small intestine and the colon, as well as in the canalicular membranes of the liver. The staining in the liver canalicular membrane indicates that BCRP may be involved in excretion processes in the liver, similar to many other ABC transporters, e.g., P-gp and MRP2 (30) . The presence of BCRP in the small intestine and colon suggests that BCRP is involved in the regulation of uptake of substrates from the gastrointestinal tract by back-transport of substrates entering from the gut lumen. This hypothesis is strengthened by the significantly increased plasma levels of p.o. administered topotecan in wild-type or mdr1a/1b (-/-) mice in the presence of the BCRP inhibitor GF120918 (29) . Staining in the breast was observed at the apical side of some ductal epithelial cells. Staining for MRP1 has also been observed in this cell type (31) . The function of these transporters at this location remains subject to additional investigations.
Finally, BCRP was present in the endothelial layer of veins and capillaries in all of the tissues. At this moment, it is not known if BCRP contributes to transport across the endothelium. The endothelium is known to be quite permeable for several substances, which are known to pass through the endothelial layer between loosely connected endothelial cells. The endothelium in the brain is different in that the endothelial cells form tight junctions, creating the blood-brain barrier. Therefore, expression of transporter proteins at the blood-brain barrier may contribute to protection of the brain. For P-gp, such a specific role in the blood-brain barrier is well established (6) . Studies investigating the influence of BCRP in the blood-brain barrier in mice are currently ongoing in our laboratory.
The knowledge of the normal tissue distribution of BCRP, as described in this report, allows attempts to increase exposure of certain tissues. One clinical implication of our findings is that p.o. administration of BCRP substrates, e.g., topotecan and irinotecan (3) , may be more efficient when combined with an inhibitor of BCRP. This concept has been proven in mice and patients for paclitaxel, which has low oral bioavailability because of its high affinity for P-gp (32) , when administered p.o. combined with the effective P-gp blocker cyclosporin A (33 , 34) . It is well known that oral bioavailability of topotecan is relatively low and variable (30 ± 7.7%) in patients (35) . Considering previously obtained results in mice (29) and the presence of BCRP in the small intestine and colon of humans as reported in this study, this bioavailability can possibly be improved by combining oral topotecan with a BCRP inhibitor, such as GF120918 (28) . We have recently started clinical trials aiming at testing the feasibility of this approach. If feasible, this principle may work for other BCRP substrate drugs as well.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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1 Supported in part by Grants NKI 99-2060 and NKI 2000-2143 from the Dutch Cancer Society and by Grant AF.35 from the Netherlands Asthma Foundation. 
2 M. M. and G. L. S. contributed equally to this study.
3 To whom requests for reprints should be addressed, at Division of Experimental Therapy, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, the Netherlands. Phone: 31-20-5122569; Fax: 31-20-5122050; E-mail: jhm_schellens{at}nki.nl
4 The abbreviations used are: P-gp, P-glycoprotein; AEC, 3-amino-9-ethylcarbazole; ECL, enhanced chemiluminescence; BCRP, Breast Cancer Resistance Protein; RT-PCR, reverse transcription-PCR; HRP, horseradish peroxidase; Mab, monoclonal antibody; MDR, multidrug-resistance; MRP, MDR Protein; PBGD, porphobilinogen deaminase; PBS/BSA, 1% BSA in PBS; nt, nucleotide.
Received 9/ 7/00. Accepted 2/13/01.
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S. Velamakanni, T. Janvilisri, S. Shahi, and H. W. van Veen A Functional Steroid-Binding Element in an ATP-Binding Cassette Multidrug Transporter Mol. Pharmacol., January 1, 2008; 73(1): 12 - 17. [Abstract] [Full Text] [PDF]
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Y. Zhang, H. Wang, J. D. Unadkat, and Q. Mao Breast Cancer Resistance Protein 1 Limits Fetal Distribution of Nitrofurantoin in the Pregnant Mouse Drug Metab. Dispos., December 1, 2007; 35(12): 2154 - 2158. [Abstract] [Full Text] [PDF]
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S. Shukla, C.-P. Wu, K. Nandigama, and S. V. Ambudkar The naphthoquinones, vitamin K3 and its structural analogue plumbagin, are substrates of the multidrug resistance linked ATP binding cassette drug transporter ABCG2 Mol. Cancer Ther., December 1, 2007; 6(12): 3279 - 3286. [Abstract] [Full Text] [PDF]
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N. Mizuno, T. Takahashi, H. Kusuhara, J. D. Schuetz, T. Niwa, and Y. Sugiyama Evaluation of the Role of Breast Cancer Resistance Protein (BCRP/ABCG2) and Multidrug Resistance-Associated Protein 4 (MRP4/ABCC4) in The Urinary Excretion of Sulfate and Glucuronide Metabolites of Edaravone (MCI-186; 3-Methyl-1-phenyl-2-pyrazolin-5-one) Drug Metab. Dispos., November 1, 2007; 35(11): 2045 - 2052. [Abstract] [Full Text] [PDF]
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N. A. de Vries, J. Zhao, E. Kroon, T. Buckle, J. H. Beijnen, and O. van Tellingen P-Glycoprotein and Breast Cancer Resistance Protein: Two Dominant Transporters Working Together in Limiting the Brain Penetration of Topotecan Clin. Cancer Res., November 1, 2007; 13(21): 6440 - 6449. [Abstract] [Full Text] [PDF]
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P. Matsson, G. Englund, G. Ahlin, C. A. S. Bergstrom, U. Norinder, and P. Artursson A Global Drug Inhibition Pattern for the Human ATP-Binding Cassette Transporter Breast Cancer Resistance Protein (ABCG2) J. Pharmacol. Exp. Ther., October 1, 2007; 323(1): 19 - 30. [Abstract] [Full Text] [PDF]
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T. Ando, H. Kusuhara, G. Merino, A. I. Alvarez, A. H. Schinkel, and Y. Sugiyama Involvement of Breast Cancer Resistance Protein (ABCG2) in the Biliary Excretion Mechanism of Fluoroquinolones Drug Metab. Dispos., October 1, 2007; 35(10): 1873 - 1879. [Abstract] [Full Text] [PDF]
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J. Enokizono, H. Kusuhara, and Y. Sugiyama Effect of Breast Cancer Resistance Protein (Bcrp/Abcg2) on the Disposition of Phytoestrogens Mol. Pharmacol., October 1, 2007; 72(4): 967 - 975. [Abstract] [Full Text] [PDF]
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N. S. Sabeva, E. J. Rouse, and G. A. Graf Defects in the Leptin Axis Reduce Abundance of the ABCG5-ABCG8 Sterol Transporter in Liver J. Biol. Chem., August 3, 2007; 282(31): 22397 - 22405. [Abstract] [Full Text] [PDF]
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N. Mizuno, T. Takahashi, Y. Iwase, H. Kusuhara, T. Niwa, and Y. Sugiyama Human Organic Anion Transporters 1 (hOAT1/SLC22A6) and 3 (hOAT3/SLC22A8) Transport Edaravone (MCI-186; 3-methyl-1-phenyl-2-pyrazolin-5-one) and Its Sulfate Conjugate Drug Metab. Dispos., August 1, 2007; 35(8): 1429 - 1434. [Abstract] [Full Text] [PDF]
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 L.-L. Hu, X.-X. Wang, X. Chen, J. Chang, C. Li, Y. Zhang, J. Yang, W. Jiang, and S.-M. Zhuang BCRP gene polymorphisms are associated with susceptibility and survival of diffuse large B-cell lymphoma Carcinogenesis, August 1, 2007; 28(8): 1740 - 1744. [Abstract] [Full Text] [PDF]
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G. Pan, N. Giri, and W. F. Elmquist Abcg2/Bcrp1 Mediates the Polarized Transport of Antiretroviral Nucleosides Abacavir and Zidovudine Drug Metab. Dispos., July 1, 2007; 35(7): 1165 - 1173. [Abstract] [Full Text] [PDF]
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T. Yamagata, H. Kusuhara, M. Morishita, K. Takayama, H. Benameur, and Y. Sugiyama Improvement of the Oral Drug Absorption of Topotecan through the Inhibition of Intestinal Xenobiotic Efflux Transporter, Breast Cancer Resistance Protein, by Excipients Drug Metab. Dispos., July 1, 2007; 35(7): 1142 - 1148. [Abstract] [Full Text] [PDF]
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J. Enokizono, H. Kusuhara, and Y. Sugiyama Regional Expression and Activity of Breast Cancer Resistance Protein (Bcrp/Abcg2) in Mouse Intestine: Overlapping Distribution with Sulfotransferases Drug Metab. Dispos., June 1, 2007; 35(6): 922 - 928. [Abstract] [Full Text] [PDF]
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R. W. Robey, S. Shukla, K. Steadman, T. Obrzut, E. M. Finley, S. V. Ambudkar, and S. E. Bates Inhibition of ABCG2-mediated transport by protein kinase inhibitors with a bisindolylmaleimide or indolocarbazole structure Mol. Cancer Ther., June 1, 2007; 6(6): 1877 - 1885. [Abstract] [Full Text] [PDF]
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I. E.L.M. Kuppens, E. O. Witteveen, R. C. Jewell, S. A. Radema, E. M. Paul, S. G. Mangum, J. H. Beijnen, E. E. Voest, and J. H.M. Schellens A Phase I, Randomized, Open-Label, Parallel-Cohort, Dose-Finding Study of Elacridar (GF120918) and Oral Topotecan in Cancer Patients Clin. Cancer Res., June 1, 2007; 13(11): 3276 - 3285. [Abstract] [Full Text] [PDF]
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J. Xu, H. Peng, Q. Chen, Y. Liu, Z. Dong, and J.-T. Zhang Oligomerization Domain of the Multidrug Resistance-Associated Transporter ABCG2 and Its Dominant Inhibitory Activity Cancer Res., May 1, 2007; 67(9): 4373 - 4381. [Abstract] [Full Text] [PDF]
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W. Liu, M. R. Baer, M. J. Bowman, P. Pera, X. Zheng, J. Morgan, R. A. Pandey, and A. R. Oseroff The Tyrosine Kinase Inhibitor Imatinib Mesylate Enhances the Efficacy of Photodynamic Therapy by Inhibiting ABCG2 Clin. Cancer Res., April 15, 2007; 13(8): 2463 - 2470. [Abstract] [Full Text] [PDF]
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C. Q. Xia, N. Liu, G. T. Miwa, and L.-S. Gan Interactions of Cyclosporin A with Breast Cancer Resistance Protein Drug Metab. Dispos., April 1, 2007; 35(4): 576 - 582. [Abstract] [Full Text] [PDF]
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 B. Ebert, A. Seidel, and A. Lampen Phytochemicals Induce Breast Cancer Resistance Protein in Caco-2 Cells and Enhance the Transport of Benzo[a]pyrene-3-sulfate Toxicol. Sci., April 1, 2007; 96(2): 227 - 236. [Abstract] [Full Text] [PDF]
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M. Wolde, A. Fellows, J. Cheng, A. Kivenson, B. Coutermarsh, L. Talebian, K. Karlson, A. Piserchio, D. F. Mierke, B. A. Stanton, et al. Targeting CAL as a Negative Regulator of {Delta}F508-CFTR Cell-Surface Expression: AN RNA INTERFERENCE AND STRUCTURE-BASED MUTAGENETIC APPROACH J. Biol. Chem., March 16, 2007; 282(11): 8099 - 8109. [Abstract] [Full Text] [PDF]
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J. Enokizono, H. Kusuhara, and Y. Sugiyama Involvement of Breast Cancer Resistance Protein (BCRP/ABCG2) in the Biliary Excretion and Intestinal Efflux of Troglitazone Sulfate, the Major Metabolite of Troglitazone with a Cholestatic Effect Drug Metab. Dispos., February 1, 2007; 35(2): 209 - 214. [Abstract] [Full Text] [PDF]
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M. Grube, S. Reuther, H. Meyer zu Schwabedissen, K. Kock, K. Draber, C. A. Ritter, C. Fusch, G. Jedlitschky, and H. K. Kroemer Organic Anion Transporting Polypeptide 2B1 and Breast Cancer Resistance Protein Interact in the Transepithelial Transport of Steroid Sulfates in Human Placenta Drug Metab. Dispos., January 1, 2007; 35(1): 30 - 35. [Abstract] [Full Text] [PDF]
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P. Breedveld, D. Pluim, G. Cipriani, F. Dahlhaus, M. A. J. van Eijndhoven, C. J. F. de Wolf, A. Kuil, J. H. Beijnen, G. L. Scheffer, G. Jansen, et al. The Effect of Low pH on Breast Cancer Resistance Protein (ABCG2)-Mediated Transport of Methotrexate, 7-Hydroxymethotrexate, Methotrexate Diglutamate, Folic Acid, Mitoxantrone, Topotecan, and Resveratrol in In Vitro Drug Transport Models Mol. Pharmacol., January 1, 2007; 71(1): 240 - 249. [Abstract] [Full Text] [PDF]
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 H. Wang, X. Wu, K. Hudkins, A. Mikheev, H. Zhang, A. Gupta, J. D. Unadkat, and Q. Mao Expression of the breast cancer resistance protein (Bcrp1/Abcg2) in tissues from pregnant mice: effects of pregnancy and correlations with nuclear receptors. Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1295 - E1304. [Abstract] [Full Text] [PDF]
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J. G. Turner, J. L. Gump, C. Zhang, J. M. Cook, D. Marchion, L. Hazlehurst, P. Munster, M. J. Schell, W. S. Dalton, and D. M. Sullivan ABCG2 expression, function, and promoter methylation in human multiple myeloma Blood, December 1, 2006; 108(12): 3881 - 3889. [Abstract] [Full Text] [PDF]
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 K. K. W. To, Z. Zhan, and S. E. Bates Aberrant Promoter Methylation of the ABCG2 Gene in Renal Carcinoma Mol. Cell. Biol., November 15, 2006; 26(22): 8572 - 8585. [Abstract] [Full Text] [PDF]
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 B. Sarkadi, L. Homolya, G. Szakacs, and A. Varadi Human Multidrug Resistance ABCB and ABCG Transporters: Participation in a Chemoimmunity Defense System. Physiol Rev, October 1, 2006; 86(4): 1179 - 1236. [Abstract] [Full Text] [PDF]
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M. J. Zamek-Gliszczynski, K. A. Hoffmaster, J. E. Humphreys, X. Tian, K.-i. Nezasa, and K. L. R. Brouwer Differential Involvement of Mrp2 (Abcc2) and Bcrp (Abcg2) in Biliary Excretion of 4-Methylumbelliferyl Glucuronide and Sulfate in the Rat J. Pharmacol. Exp. Ther., October 1, 2006; 319(1): 459 - 467. [Abstract] [Full Text] [PDF]
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J. A. Seamon, C. A. Rugg, S. Emanuel, A. M. Calcagno, S. V. Ambudkar, S. A. Middleton, J. Butler, V. Borowski, and L. M. Greenberger Role of the ABCG2 drug transporter in the resistance and oral bioavailability of a potent cyclin-dependent kinase/Aurora kinase inhibitor. Mol. Cancer Ther., October 1, 2006; 5(10): 2459 - 2467. [Abstract] [Full Text] [PDF]
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 S. Vander Borght, L. Libbrecht, A. Katoonizadeh, J. van Pelt, D. Cassiman, F. Nevens, A. Van Lommel, B. E. Petersen, J. Fevery, P. L. Jansen, et al. Breast Cancer Resistance Protein (BCRP/ABCG2) Is Expressed by Progenitor Cells/Reactive Ductules and Hepatocytes and Its Expression Pattern Is Influenced by Disease Etiology and Species Type: Possible Functional Consequences J. Histochem. Cytochem., September 1, 2006; 54(9): 1051 - 1059. [Abstract] [Full Text] [PDF]
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I. Szatmari, G. Vamosi, P. Brazda, B. L. Balint, S. Benko, L. Szeles, V. Jeney, C. Ozvegy-Laczka, A. Szanto, E. Barta, et al. Peroxisome Proliferator-activated Receptor {gamma}-regulated ABCG2 Expression Confers Cytoprotection to Human Dendritic Cells J. Biol. Chem., August 18, 2006; 281(33): 23812 - 23823. [Abstract] [Full Text] [PDF]
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N. E. Jordanides, H. G. Jorgensen, T. L. Holyoake, and J. C. Mountford Functional ABCG2 is overexpressed on primary CML CD34+ cells and is inhibited by imatinib mesylate Blood, August 15, 2006; 108(4): 1370 - 1373. [Abstract] [Full Text] [PDF]
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 A. Kobayashi, Y. Takanezawa, T. Hirata, Y. Shimizu, K. Misasa, N. Kioka, H. Arai, K. Ueda, and M. Matsuo Efflux of sphingomyelin, cholesterol, and phosphatidylcholine by ABCG1 J. Lipid Res., August 1, 2006; 47(8): 1791 - 1802. [Abstract] [Full Text] [PDF]
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W. Chearwae, S. Shukla, P. Limtrakul, and S. V. Ambudkar Modulation of the function of the multidrug resistance-linked ATP-binding cassette transporter ABCG2 by the cancer chemopreventive agent curcumin. Mol. Cancer Ther., August 1, 2006; 5(8): 1995 - 2006. [Abstract] [Full Text] [PDF]
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T. Nakanishi, K. Shiozawa, B. A. Hassel, and D. D. Ross Complex interaction of BCRP/ABCG2 and imatinib in BCR-ABL-expressing cells: BCRP-mediated resistance to imatinib is attenuated by imatinib-induced reduction of BCRP expression Blood, July 15, 2006; 108(2): 678 - 684. [Abstract] [Full Text] [PDF]
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 S. Choudhuri and C. D. Klaassen Structure, Function, Expression, Genomic Organization, and Single Nucleotide Polymorphisms of Human ABCB1 (MDR1), ABCC (MRP), and ABCG2 (BCRP) Efflux Transporters International Journal of Toxicology, July 1, 2006; 25(4): 231 - 259. [Abstract] [Full Text] [PDF]
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R. G. Deeley, C. Westlake, and S. P. C. Cole Transmembrane Transport of Endo- and Xenobiotics by Mammalian ATP-Binding Cassette Multidrug Resistance Proteins. Physiol Rev, July 1, 2006; 86(3): 849 - 899. [Abstract] [Full Text] [PDF]
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 A Mohan, M Kandalam, H L Ramkumar, L Gopal, and S Krishnakumar Stem cell markers: ABCG2 and MCM2 expression in retinoblastoma Br J Ophthalmol, July 1, 2006; 90(7): 889 - 893. [Abstract] [Full Text] [PDF]
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M. Leggas, J. C. Panetta, Y. Zhuang, J. D. Schuetz, B. Johnston, F. Bai, B. Sorrentino, S. Zhou, P. J. Houghton, and C. F. Stewart Gefitinib Modulates the Function of Multiple ATP-Binding Cassette Transporters In vivo. Cancer Res., May 1, 2006; 66(9): 4802 - 4807. [Abstract] [Full Text] [PDF]
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H. Wang, L. Zhou, A. Gupta, R. R. Vethanayagam, Y. Zhang, J. D. Unadkat, and Q. Mao Regulation of BCRP/ABCG2 expression by progesterone and 17beta-estradiol in human placental BeWo cells Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E798 - E807. [Abstract] [Full Text] [PDF]
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H. E. M. zu Schwabedissen, M. Grube, A. Dreisbach, G. Jedlitschky, K. Meissner, K. Linnemann, C. Fusch, C. A. Ritter, U. Volker, and H. K. Kroemer EPIDERMAL GROWTH FACTOR-MEDIATED ACTIVATION OF THE MAP KINASE CASCADE RESULTS IN ALTERED EXPRESSION AND FUNCTION OF ABCG2 (BCRP) Drug Metab. Dispos., April 1, 2006; 34(4): 524 - 533. [Abstract] [Full Text] [PDF]
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K.-i. Nezasa, X. Tian, M. J. Zamek-Gliszczynski, N. J. Patel, T. J. Raub, and K. L. R. Brouwer ALTERED HEPATOBILIARY DISPOSITION OF 5 (AND 6)-CARBOXY-2',7'-DICHLOROFLUORESCEIN IN Abcg2 (Bcrp1) AND Abcc2 (Mrp2) KNOCKOUT MICE Drug Metab. Dispos., April 1, 2006; 34(4): 718 - 723. [Abstract] [Full Text] [PDF]
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K. Meissner, B. Heydrich, G. Jedlitschky, H. Meyer zu Schwabedissen, I. Mosyagin, P. Dazert, L. Eckel, S. Vogelgesang, R. W. Warzok, M. Bohm, et al. The ATP-binding Cassette Transporter ABCG2 (BCRP), a Marker for Side Population Stem Cells, Is Expressed in Human Heart J. Histochem. Cytochem., February 1, 2006; 54(2): 215 - 221. [Abstract] [Full Text] [PDF]
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A. Swiatecka-Urban, A. Brown, S. Moreau-Marquis, J. Renuka, B. Coutermarsh, R. Barnaby, K. H. Karlson, T. R. Flotte, M. Fukuda, G. M. Langford, et al. The Short Apical Membrane Half-life of Rescued {Delta}F508-Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Results from Accelerated Endocytosis of {Delta}F508-CFTR in Polarized Human Airway Epithelial Cells J. Biol. Chem., November 4, 2005; 280(44): 36762 - 36772. [Abstract] [Full Text] [PDF]
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U. Henriksen, J. U. Fog, T. Litman, and U. Gether Identification of Intra- and Intermolecular Disulfide Bridges in the Multidrug Resistance Transporter ABCG2 J. Biol. Chem., November 4, 2005; 280(44): 36926 - 36934. [Abstract] [Full Text] [PDF]
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B. Ebert, A. Seidel, and A. Lampen Identification of BCRP as transporter of benzo[a]pyrene conjugates metabolically formed in Caco-2 cells and its induction by Ah-receptor agonists Carcinogenesis, October 1, 2005; 26(10): 1754 - 1763. [Abstract] [Full Text] [PDF]
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A. Shafran, I. Ifergan, E. Bram, G. Jansen, I. Kathmann, G. J. Peters, R. W. Robey, S. E. Bates, and Y. G. Assaraf ABCG2 Harboring the Gly482 Mutation Confers High-Level Resistance to Various Hydrophilic Antifolates Cancer Res., September 15, 2005; 65(18): 8414 - 8422. [Abstract] [Full Text] [PDF]
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S. Matsushima, K. Maeda, C. Kondo, M. Hirano, M. Sasaki, H. Suzuki, and Y. Sugiyama Identification of the Hepatic Efflux Transporters of Organic Anions Using Double-Transfected Madin-Darby Canine Kidney II Cells Expressing Human Organic Anion-Transporting Polypeptide 1B1 (OATP1B1)/Multidrug Resistance-Associated Protein 2, OATP1B1/Multidrug Resistance 1, and OATP1B1/Breast Cancer Resistance Protein J. Pharmacol. Exp. Ther., September 1, 2005; 314(3): 1059 - 1067. [Abstract] [Full Text] [PDF]
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M. Hirano, K. Maeda, S. Matsushima, Y. Nozaki, H. Kusuhara, and Y. Sugiyama Involvement of BCRP (ABCG2) in the Biliary Excretion of Pitavastatin Mol. Pharmacol., September 1, 2005; 68(3): 800 - 807. [Abstract] [Full Text] [PDF]
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M. J. Zamek-Gliszczynski, K. A. Hoffmaster, X. Tian, R. Zhao, J. W. Polli, J. E. Humphreys, L. O. Webster, A. S. Bridges, J. C. Kalvass, and K. L. R. Brouwer MULTIPLE MECHANISMS ARE INVOLVED IN THE BILIARY EXCRETION OF ACETAMINOPHEN SULFATE IN THE RAT: ROLE OF MRP2 AND BCRP1 Drug Metab. Dispos., August 1, 2005; 33(8): 1158 - 1165. [Abstract] [Full Text] [PDF]
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X.-f. Zhou, X. Yang, Q. Wang, R. A. Coburn, and M. E. Morris EFFECTS OF DIHYDROPYRIDINES AND PYRIDINES ON MULTIDRUG RESISTANCE MEDIATED BY BREAST CANCER RESISTANCE PROTEIN: IN VITRO AND IN VIVO STUDIES Drug Metab. Dispos., August 1, 2005; 33(8): 1220 - 1228. [Abstract] [Full Text] [PDF]
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H. E. Meyer zu Schwabedissen, G. Jedlitschky, M. Gratz, S. Haenisch, K. Linnemann, C. Fusch, I. Cascorbi, and H. K. Kroemer VARIABLE EXPRESSION OF MRP2 (ABCC2) IN HUMAN PLACENTA: INFLUENCE OF GESTATIONAL AGE AND CELLULAR DIFFERENTIATION Drug Metab. Dispos., July 1, 2005; 33(7): 896 - 904. [Abstract] [Full Text] [PDF]
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R. R. Vethanayagam, H. Wang, A. Gupta, Y. Zhang, F. Lewis, J. D. Unadkat, and Q. Mao FUNCTIONAL ANALYSIS OF THE HUMAN VARIANTS OF BREAST CANCER RESISTANCE PROTEIN: I206L, N590Y, AND D620N Drug Metab. Dispos., June 1, 2005; 33(6): 697 - 705. [Abstract] [Full Text] [PDF]
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A. L. A. Sesink, I. C. W. Arts, V. C. J. de Boer, P. Breedveld, J. H. M. Schellens, P. C. H. Hollman, and F. G. M. Russel Breast Cancer Resistance Protein (Bcrp1/Abcg2) Limits Net Intestinal Uptake of Quercetin in Rats by Facilitating Apical Efflux of Glucuronides Mol. Pharmacol., June 1, 2005; 67(6): 1999 - 2006. [Abstract] [Full Text] [PDF]
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 N. Zelcer, K. van de Wetering, M. Hillebrand, E. Sarton, A. Kuil, P. R. Wielinga, T. Tephly, A. Dahan, J. H. Beijnen, and P. Borst Mice lacking multidrug resistance protein 3 show altered morphine pharmacokinetics and morphine-6-glucuronide antinociception PNAS, May 17, 2005; 102(20): 7274 - 7279. [Abstract] [Full Text] [PDF]
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C. Q. Xia, N. Liu, D. Yang, G. Miwa, and L.-S. Gan EXPRESSION, LOCALIZATION, AND FUNCTIONAL CHARACTERISTICS OF BREAST CANCER RESISTANCE PROTEIN IN CACO-2 CELLS Drug Metab. Dispos., May 1, 2005; 33(5): 637 - 643. [Abstract] [Full Text] [PDF]
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 M. T. Budak, O. S. Alpdogan, M. Zhou, R. M. Lavker, M.A. M. Akinci, and J. M. Wolosin Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells J. Cell Sci., April 15, 2005; 118(8): 1715 - 1724. [Abstract] [Full Text] [PDF]
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D. Kolwankar, D. D. Glover, J. A. Ware, and T. S. Tracy EXPRESSION AND FUNCTION OF ABCB1 AND ABCG2 IN HUMAN PLACENTAL TISSUE Drug Metab. Dispos., April 1, 2005; 33(4): 524 - 529. [Abstract] [Full Text] [PDF]
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U. Henriksen, U. Gether, and T. Litman Effect of Walker A mutation (K86M) on oligomerization and surface targeting of the multidrug resistance transporter ABCG2 J. Cell Sci., April 1, 2005; 118(7): 1417 - 1426. [Abstract] [Full Text] [PDF]
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I. Ifergan, G. Jansen, and Y. G. Assaraf Cytoplasmic Confinement of Breast Cancer Resistance Protein (BCRP/ABCG2) as a Novel Mechanism of Adaptation to Short-Term Folate Deprivation Mol. Pharmacol., April 1, 2005; 67(4): 1349 - 1359. [Abstract] [Full Text] [PDF]
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M. H.G.P. Raaijmakers, E. P.L.M. de Grouw, L. H.H. Heuver, B. A. van der Reijden, J. H. Jansen, R. J. Scheper, G. L. Scheffer, T. J.M. de Witte, and R. A.P. Raymakers Breast Cancer Resistance Protein in Drug Resistance of Primitive CD34+38- Cells in Acute Myeloid Leukemia Clin. Cancer Res., March 15, 2005; 11(6): 2436 - 2444. [Abstract] [Full Text] [PDF]
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S. Zhou, Y. Zong, P. A. Ney, G. Nair, C. F. Stewart, and B. P. Sorrentino Increased expression of the Abcg2 transporter during erythroid maturation plays a role in decreasing cellular protoporphyrin IX levels Blood, March 15, 2005; 105(6): 2571 - 2576. [Abstract] [Full Text] [PDF]
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S. Zhang, X. Wang, K. Sagawa, and M. E. Morris FLAVONOIDS CHRYSIN AND BENZOFLAVONE, POTENT BREAST CANCER RESISTANCE PROTEIN INHIBITORS, HAVE NO SIGNIFICANT EFFECT ON TOPOTECAN PHARMACOKINETICS IN RATS OR MDR1A/1B (-/-) MICE Drug Metab. Dispos., March 1, 2005; 33(3): 341 - 348. [Abstract] [Full Text] [PDF]
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K. F. K. Ejendal and C. A. Hrycyna Differential Sensitivities of the Human ATP-Binding Cassette Transporters ABCG2 and P-Glycoprotein to Cyclosporin A Mol. Pharmacol., March 1, 2005; 67(3): 902 - 911. [Abstract] [Full Text] [PDF]
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Y. Nakamura, M. Oka, H. Soda, K. Shiozawa, M. Yoshikawa, A. Itoh, Y. Ikegami, J. Tsurutani, K. Nakatomi, T. Kitazaki, et al. Gefitinib ("Iressa", ZD1839), an Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor, Reverses Breast Cancer Resistance Protein/ABCG2-Mediated Drug Resistance Cancer Res., February 15, 2005; 65(4): 1541 - 1546. [Abstract] [Full Text] [PDF]
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C. Ozvegy-Laczka, G. Varady, G. Koblos, O. Ujhelly, J. Cervenak, J. D. Schuetz, B. P. Sorrentino, G.-J. Koomen, A. Varadi, K. Nemet, et al. Function-dependent Conformational Changes of the ABCG2 Multidrug Transporter Modify Its Interaction with a Monoclonal Antibody on the Cell Surface J. Biol. Chem., February 11, 2005; 280(6): 4219 - 4227. [Abstract] [Full Text] [PDF]
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Y. Imai, E. Ishikawa, S. Asada, and Y. Sugimoto Estrogen-Mediated Post transcriptional Down-regulation of Breast Cancer Resistance Protein/ABCG2 Cancer Res., January 15, 2005; 65(2): 596 - 604. [Abstract] [Full Text] [PDF]
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D. Kobayashi, I. Ieiri, T. Hirota, H. Takane, S. Maegawa, J. Kigawa, H. Suzuki, E. Nanba, M. Oshimura, N. Terakawa, et al. FUNCTIONAL ASSESSMENT OF ABCG2 (BCRP) GENE POLYMORPHISMS TO PROTEIN EXPRESSION IN HUMAN PLACENTA Drug Metab. Dispos., January 1, 2005; 33(1): 94 - 101. [Abstract] [Full Text] [PDF]
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P. Pavek, G. Merino, E. Wagenaar, E. Bolscher, M. Novotna, J. W. Jonker, and A. H. Schinkel Human Breast Cancer Resistance Protein: Interactions with Steroid Drugs, Hormones, the Dietary Carcinogen 2-Amino-1-methyl-6-phenylimidazo(4,5-b)pyridine, and Transport of Cimetidine J. Pharmacol. Exp. Ther., January 1, 2005; 312(1): 144 - 152. [Abstract] [Full Text] [PDF]
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 H. E.U. Meyer zu Schwabedissen, M. Grube, B. Heydrich, K. Linnemann, C. Fusch, H. K. Kroemer, and G. Jedlitschky Expression, Localization, and Function of MRP5 (ABCC5), a Transporter for Cyclic Nucleotides, in Human Placenta and Cultured Human Trophoblasts: Effects of Gestational Age and Cellular Differentiation Am. J. Pathol., January 1, 2005; 166(1): 39 - 48. [Abstract] [Full Text] [PDF]
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P.L. R. Ee, X. He, D. D. Ross, and W. T. Beck Modulation of breast cancer resistance protein (BCRP/ABCG2) gene expression using RNA interference Mol. Cancer Ther., December 1, 2004; 3(12): 1577 - 1584. [Abstract] [Full Text] [PDF]
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F. Loganzo, M. Hari, T. Annable, X. Tan, D. B. Morilla, S. Musto, A. Zask, J. Kaplan, A. A. Minnick Jr., M. K. May, et al. Cells resistant to HTI-286 do not overexpress P-glycoprotein but have reduced drug accumulation and a point mutation in {alpha}-tubulin Mol. Cancer Ther., October 1, 2004; 3(10): 1319 - 1327. [Abstract] [Full Text] [PDF]
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N. Mizuno, M. Suzuki, H. Kusuhara, H. Suzuki, K. Takeuchi, T. Niwa, J. W. Jonker, and Y. Sugiyama IMPAIRED RENAL EXCRETION OF 6-HYDROXY-5,7-DIMETHYL-2-METHYLAMINO-4-(3-PYRIDYLMETHYL) BENZOTHIAZOLE (E3040) SULFATE IN BREAST CANCER RESISTANCE PROTEIN (BCRP1/ABCG2) KNOCKOUT MICE Drug Metab. Dispos., September 1, 2004; 32(9): 898 - 901. [Abstract] [Full Text] [PDF]
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K. Yanase, S. Tsukahara, S. Asada, E. Ishikawa, Y. Imai, and Y. Sugimoto Gefitinib reverses breast cancer resistance protein-mediated drug resistance Mol. Cancer Ther., September 1, 2004; 3(9): 1119 - 1125. [Abstract] [Full Text] [PDF]
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F. A. de Jong, S. Marsh, R. H. J. Mathijssen, C. King, J. Verweij, A. Sparreboom, and H. L. McLeod ABCG2 Pharmacogenetics: Ethnic Differences in Allele Frequency and Assessment of Influence on Irinotecan Disposition Clin. Cancer Res., September 1, 2004; 10(17): 5889 - 5894. [Abstract] [Full Text] [PDF]
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