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Tumor Biology |
Cancer Research U.K., Translational Oncology Laboratory, Bart’s and the London, Queen Mary’s School of Medicine and Dentistry, London EC1M 6BQ, United Kingdom [C. J. S., J. L. W., K. S., G. D. W., F. R. B.]; Department of Histopathology, Division of Investigative Sciences, Imperial College Faculty of Medicine, Hammersmith Hospital Campus, Du Cane Road, London W12 ONN, United Kingdom [G. S.]; Departments of Obstetrics and Gynaecology and Pathology, University of South Florida, Tampa, Florida 33606 [G. D. W.]; and AnorMED, Inc., Langley, British Columbia, V2Y 1N5, Canada [S. F., G. B.]
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ABSTRACT |
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 Top ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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, a cytokine that is expressed by tumor cells in ovarian cancer biopsies. IGROV cells invaded through Matrigel toward a CXCL12 gradient. Invasion was abrogated by the broad spectrum matrix metalloproteinase and TNF converting enzyme inhibitor Marimastat and was partially inhibited by neutralizing antitumor necrosis factor antibodies. These effects were not limited to the IGROV cell line. They could also be demonstrated in the CAOV-3 ovarian cancer cell line and primary ovarian tumor cells isolated from ovarian ascites. These biological effects of CXCL12 on IGROV cells were also inhibited by the small molecular weight CXCR4 antagonist AMD3100. Finally, we found abundant intracellular CXCL12 protein in tumor cells in 15 of 18 ovarian cancer biopsies but not in epithelial cells from normal ovary or borderline disease. The chemokine CXCL12 may have multiple biological effects in ovarian cancer, stimulating cell migration and invasion through extracellular matrix, as well as DNA synthesis and establishment of a cytokine network in situations that are suboptimal for tumor cell growth. |
INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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However, chemokines may play other roles in cancer. Some are potent angiogenic factors, whereas others can be angiostatic; alterations in the chemokine balance may contribute to the development of the tumor vasculature (8 , 9) .
In addition, the restricted expression of chemokine receptors, especially CXCR4 and CCR7, by tumor cells, may be one important step in the development of site-specific metastasis (8 , 10, 11, 12, 13, 14, 15, 16, 17) . Tumor cells from breast, prostate, pancreatic and ovarian carcinomas, neuroblastoma, glioblastoma, and some leukemias express chemokine receptors. In breast, prostate and ovarian cancer, neuroblastoma, and leukemia, the respective ligand is expressed at sites of tumor spread. When B16 melanoma cells were transduced with a retroviral vector containing cDNA for the chemokine receptor CCR7, metastasis to lymph nodes was increased; these results provided experimental proof that cancer cells may co-opt normal mechanisms of leukocyte homing to lymph nodes (18) .
As described above, we recently reported that CXCR4 was the only one of 14 chemokine receptors investigated that was expressed on a subset of tumor cells in ovarian neoplasms (10)
. Stimulation of CXCR4 induced a calcium flux and directed migration of the tumor cells. Moreover, CXCR4 was expressed on a subset of cells in primary ovarian tumors and its ligand CXCL12 (stromal cell-derived factor 1) was found in nanogram quantities in ascitic fluid from ovarian cancer patients. In this report, we have further studied the role of the CXCR4 receptor on ovarian tumor cell behavior. We report that stimulation of this chemokine receptor with CXCL12 also promotes invasion of cells through extracellular matrix, production of the proinflammatory cytokine TNF-3
, and stimulates cell growth. These actions may allow tumor cells to grow in suboptimal conditions and initiate a cytokine network in the surrounding stroma. The relevance of this is shown by the abundant and tumor-specific expression of CXCL12 in ovarian epithelium. Taken together with recent observations on the role of tumor cell CXCL12 in attracting tumor-promoting pre-DC2 cells into the microenvironment of human ovarian cancer (19)
, our data suggest that CXCR4/CXCL12 may provide important paracrine and autocrine signals that promote malignant progression in ovarian cancer.
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MATERIALS AND METHODS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Immunohistochemistry.
Using a standard avidin-biotin complex technique, a rabbit polyclonal mouse anti-CXCL12 antibody (Peprotech, London, United Kingdom) was used at a dilution of 1 in 250 and localized with 3,3'-diaminobenzidine, followed by counterstaining with Toluidine blue dye. Sections were scored ± if staining was equivocal, exhibiting either pale, heterogeneous, or barely above background and + where there was definite, consistent, and appropriate immunoreactivity. Appropriate controls were performed with omission of the primary antibody and also using preimmune serum before secondary reagents. No nonspecific staining was detected.
Cell Lines.
The ovarian cancer cell line IGROV (20)
was cultured in pyrogen-free conditions in RPMI 1640 supplemented with 10% FCS, and the CAOV-3 ovarian cancer cell line (American Type Culture Collection) was cultured in DMEM supplemented with 10% FCS. To allow recovery of cells after trypsinization, cells were cultured as single cell suspensions in Teflon-coated pots (Tuf Tainer; Perbio Science, United Kingdom Ltd., United Kingdom) at 0.5–1 x 106 cells/ml. IGROV cells were usually treated with 100 ng/ml CXCL12 (Peprotech). This concentration was previously shown to stimulate calcium flux and migration of these cells (10)
. In some experiments, concentrations of 10 and 50 ng/ml CXCL12 were also used.
Isolation of Primary Ovarian Tumor Cells from Ascitic Fluid.
Samples of ascitic fluid were collected from patients with ovarian carcinoma at the time of surgery or by paracentesis for palliative/diagnostic purposes. Each sample was spun down, and the cell isolate was treated with erythrocyte lysis buffer to remove RBCs. Tumor cells expressing the human epithelial antigen were isolated using MACS HEA Microbeads (Miltenyi Biotec, Bisley, Surrey, United Kingdom) according to the manufacturer’s instructions.
Proliferation Assays.
Cells (2 x 104) were plated in 24-well plates and cultured for 18 h before replacing the media with RPMI 1640 supplemented with 1% BSA (Sigma, Poole, United Kingdom) ± 100 ng/ml CXCL12 (Peprotech). In some experiments, 1 µg/ml anti-TNF- mAb (R&D Systems, Abingdon, United Kingdom), 20 ng/ml CCL2 (Peprotech), or 20 µg/ml anti-CXCR4 mAb (R&D Systems) were added. Cells were harvested by trypsinization and counted using trypan blue exclusion with a hemocytometer. Cells were cultured for 40 h in the presence of 1 µCi/ml [3H]thymidine (TRA61; Amersham Pharmacia Biotech, Amersham, United Kingdom). The radioactivity incorporated into the acid insoluble material was measured using liquid scintillation counting.
Apoptosis Assay.
IGROV cells (2 x 105) were cultured in Teflon pots overnight before stimulation with 100 ng/ml CXCL12. Apoptosis was measured using the Cell Death Detection ELISA (Roche Molecular Biologicals, Mannheim, Germany) according to the manufacturer’s instructions.
Western Blotting.
Cells (3 x 106) were cultured overnight in Teflon pots before stimulation with CXCL12. Cell lysates were prepared by adding ice-cold lysis buffer [50 mM Tris (pH 8.0), 1% Triton X-100, 100 mM NaCl, 1 mM MgCl2, 1 mM Na Van, 20 mM NaF, 25 µg/ml Aprotinin, 50 µg/ml leupeptin, 25 µg/ml pepstatin A, and 1 mM ß glycerophosphate; all from Sigma). A total of 10 µg of control or CXCL12-stimulated cell extracts was run on a SDS 10% SDS-polyacrylamide gel and transferred to a Hybond-ECL nylon membrane (Amersham). The membrane was probed using an antiphospho-AKT antibody and anti-AKT antibody (New England Biolabs, Beverly, MA) or anti-p44/42 MAPK antibody and antiphospho-p44/42 MAPK antibody (New England Biolabs) according to the manufacturer’s instructions. Western Blot Chemiluminescence Reagent Plus Kit (NEN Life Science Products, Boston, MA) was used for detection. Protein concentration equivalence was confirmed after probing by amido black staining and ß-actin antibody.
RNA Extraction and Real-Time Quantitative RT-PCR Analysis.
RNA was extracted using Tri Reagent (Sigma) and treated with 10 units of DNase (Pharmacia, Milton Keynes, United Kingdom) following the manufacturers’ instructions. DNase-treated RNA (2 µg) was reversed transcribed with Moloney murine leukemia virus reverse transcriptase (Promega, Southampton, United Kingdom) according to the manufacturer’s instructions and diluted to 100 µl with distilled water. Multiplex real-time analysis was performed using premade TNF- (FAM), and 18 s rRNA (VIC) specific primers and probes with the ABI Prism 7700 Sequence Detection System instrument and software (PE Applied Biosystems, Warrington, United Kingdom). RT-PCR was carried out with the TaqMan Universal RT-PCR Master Mix (PE Applied Biosystems) using 2.5 µl of cDNA in a 25-µl final reaction mixture. Cycling conditions were incubation at 50°C for 2 min, 10 min at 95°C, and 40 cycles of 15 s at 95°C and 1 min at 60°C. Experiments were performed in triplicate for each sample. TNF- was normalized (
Ct) to 18 s rRNA by subtracting the cycle threshold (Ct) value of 18 s rRNA from the Ct value of TNF-. Fold difference compared with control was calculated.
ELISA for TNF- in IGROV Cell Culture Supernatants.
TNF- was measured using the Quantikine TNF- ELISA kit (R&D Systems). The sensitivity of the assay was 4.4 pg/ml.
Invasion.
Invasion was assayed using Matrigel Invasion Chambers (24-well format, 8-µm pore; BD PharMingen). Medium (0.5 ml) containing 5 x 105 cells was added to the upper chamber, and 0.5 ml of either medium alone or media supplemented with 100 ng/ml CXCL12 was added to the lower chamber. Chambers were incubated overnight at 37°C and 5% CO2. Cells on the upper surface of the filter were removed using a cotton wool swab. Migrated cells on the lower surface were stained using DiffQuik (Dade Behring, Düdingen, Switzerland) before microscopic analysis. The number of migrated cells in 10 medium power fields (x20) was counted. In some experiments 1 µg/ml of a neutralizing anti-TNF- mAb (R&D Systems) was added to the cells in the upper chamber.
RT-PCR Screen for MMPs.
Total RNA and cDNA was prepared from all samples using Tri Reagent as described above. For RT-PCR, total RNA was DNase-treated to remove contaminating genomic DNA using RNase-free DNase I (Pharmacia Biotech). cDNA was synthesized from DNase-treated total RNA using the Ready-to-Go T-primed First Strand kit (Pharmacia Biotech). The primers for GAPDH were designed from sequences submitted to GenBank using Primer 3.0. The primers for MMPs, membrane-type MMPs, TIMPs, uPA, uPA receptor, and plasminogen activator inhibitor were designed by Thomas Leber, Stephen Robinson, and Kate Scott in our laboratory. The primer sequences and product sizes of the proteases and inhibitors expressed by IGROV cells are as follows in Table 1
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AMD3100.
AMD3100, a small molecular weight CXCR4 antagonist, was used at a final concentration of 1 µg/ml. Concentrations of between 100 ng and 1 µg/ml have been shown to inhibit HIV entry into cells in vitro (21)
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Statistical Analysis.
The results of the migration assays were evaluated using the Welch’s approximate t test (which is a more stringent analysis than the Student’s t test).
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Activation of Akt/PKB in IGROV Cells after CXCL12 Stimulation.
PKB is a downstream effector of PI3k and has been implicated in several signal transduction pathways, many of which promote cell survival (22)
. It is also important in the chemotactic response (23)
. IGROV cells were stimulated with 100 ng/ml CXCL12, and total cell lysates were prepared at various time points. Western blots were performed using 10 µg of total protein, and these were probed for phospho-Akt and Akt. Activation of Akt was seen in IGROV cells, with a 3–4-fold increase in phospho-Akt (relative to Akt) within 10 min. This induction of phospho-Akt was still maintained at 2 h (Fig. 1)
. Phospho-Akt (100 ng/ml) was used as this was the concentration shown in our previous publication to induce calcium flux within the cells. CXCL12 (50 ng/ml) also induced activation of Akt, whereas low concentrations of CXCL12 (10 ng/ml) did not induce detectable signaling (data not shown).
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(24)
and can lead to a variety of cell-type specific effects and gene induction. p44 and p42 MAPK (ERK1 and ERK2, respectively) are important components of this MAPK cascade. IGROV cells were stimulated with 100 ng/ml CXCL12, and total cell lysates were prepared at various time points. Western blots were performed using 10 µg of total protein, and these were probed for phospho-p44/42 MAPK, nonphosphorylated p44/42 MAPK, and ß-actin. In IGROV cells, there was approximately a 3-fold increase in the ratio of phopsho-p44/42 MAPK to p44/42 MAPK after 15 min (Fig. 2)
. The ratio then decreased back to control levels after 30 min before increasing to 4–5-fold of control levels after 2 h. These results suggest that there is biphasic MAPK signaling in IGROV cells in response to CXCL12, as has been shown for other cell types (25)
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CXCL12 Causes Proliferation of IGROV Cells.
CXCL12 was added to IGROV cells maintained in serum-free suboptimal growth conditions. There was a significant increase in IGROV cell numbers after stimulation with 100 ng/ml CXCL12 at 4 days (Fig. 3
; P < 0.005). There was also significant proliferation of IGROV cells to 50 ng/ml CXCL12 (P < 0.05), whereas lower concentrations of CXCL12 (10 ng/ml) did not significantly increase cell number (data not shown). Proliferation assays performed in Teflon pots with nonadherent cells showed a similar increase in cell number. There was no increase in cell number when IGROV cells were stimulated with 50 ng/ml of the chemokine CCL2 (data not shown). Addition of anti-CXCR4 mAb at 10 µg/ml inhibited the CXCL12-dependent increase in cell number; the anti-CXCR4 mAb had no effect on the growth of unstimulated cells. Thus, CXCR4 was necessary for the CXCL12-dependent increase in cell number, but there was no evidence for an autocrine growth loop because addition of anti-CXCR4 mAb did not decrease proliferation of IGROV cells below control levels. We found no evidence that CXCL12 was acting as a survival factor for IGROV cells. When we used the Cell Death Detection ELISA, no evidence of apoptosis was detected either in control or CXCL12-stimulated cultures (data not shown). However, CXCL12 stimulated uptake of [3H]thymidine in IGROV cells (Fig. 4)
, demonstrating that CXCL12 stimulates DNA synthesis.
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after CXCL12 Stimulation of IGROV Cells. is an important mediator of tumor:stromal cell communication in the tumor microenvironment of epithelial ovarian cancer (26)
. We have also found that chemokine (CCL1, CCL2, or CCL5) treatment of monocytic cells and macrophages induces a pulse of de novo TNF- synthesis (27)
. IGROV cells were stimulated with 100 ng/ml CXCL12 and TNF- protein, and mRNA levels were measured at various time points using ELISA and real-time quantitative TaqMan RT-PCR. TNF- mRNA was up-regulated after CXCL12 stimulation, reaching a 4-fold increase over control levels after 3 h (Fig. 5A)
. TNF- protein was not detected in control cultures, but 15pg/ml TNF- was found in the medium 8 h after CXCL12 stimulation, increasing to 32pg/ml at 24 h (Fig. 5B)
. This difference in detection may reflect the extreme sensitivity of quantitative real time RT-PCR in comparison to ELISA or may indicate a role for the membrane bound form of TNF.
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can be a growth factor for some tumor cell lines, we also added 1 µg/ml neutralizing antibody to TNF- when IGROV cells were growing in the presence of CXCL12. This concentration of anti-TNF- was used as it had been previously demonstrated in this lab to neutralize chemokine-induced TNF- and MMP-9 production by monocytes (27)
. However, neutralization of TNF- release had no effect on the CXCL12 stimulation of [3H]thymidine uptake (Fig. 4)
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CXCL12 Stimulates Ovarian Cancer Cell Invasion.
IGROV cells were also tested for their ability to invade through growth factor-reduced Matrigel invasion chambers. In response to a gradient generated by 100 ng/ml CXCL12, IGROV cells invaded through Matrigel (Fig. 6A)
. Two and 20 µM of the metallo-enzyme inhibitor Marimastat inhibited invasion toward CXCL12 (Fig. 6A)
. These doses of Marimastat had no effect on IGROV cell growth (data not shown). IGROV cells do not invade through Matrigel in response to a gradient of 20 ng/ml CCL2, a chemokine widely expressed in ovarian cancer (data not shown).
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. Treatment of IGROV cells with CXCL12 for 8 or 24 h did not induce the expression of additional MMPs (Fig. 7)
. Real-time RT-PCR was used to look for increase in abundance of MMP-7, MMP-11, and MMP-15 after CXCL12 treatment. CXCL12 does not appear to alter the expression of the MMPs that we have investigated, however, there are many more MMPs that could be affected by stimulation with CXCL12. Alternatively a cytokine downstream of CXCL12 could affect MMP expression. The induction of TNF- by CXCL12 appeared to be involved in the process of invasion. As shown in Fig. 6B
, addition of a neutralizing anti-TNF- mAb significantly inhibited CXCL12-stimulated Matrigel invasion (P < 0.002). However, IGROV and CAOV-3 cells do not migrate or invade toward 30, 50, or 100 ng/ml TNF-, and addition of these concentrations of TNF- to tumor cells in the Matrigel assay did not enhance the invasive capacity of these cells (data not shown).
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, and CAOV-3 cells were able to invade through growth factor-reduced Matrigel invasion chambers toward a CXCL12 gradient (Fig. 8D)
. CAOV-3 cells also produced TNF- after stimulation with 100 ng/ml CXCL12; there was a 2-fold increase in TNF- mRNA after 3 h of stimulation with CXCL12 (data not shown).
Primary ovarian tumor cells isolated from ascites were able to produce TNF- mRNA in response to stimulation with CXCL12 (Fig. 5C)
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AMD3100, a CXCR4 Antagonist, May Have Therapeutic Potential in Ovarian Cancer.
AMD3100 is a specific antagonist for CXCR4 that blocks HIV infection of T-tropic X4-using virus in vitro and in vivo and inhibits migration of monocytic cells toward CXCL12 (21)
. AMD3100 has been evaluated in a Phase I clinical trial in healthy volunteers and subsequently in HIV-positive patients. Treatment of the ovarian cancer cell lines with AMD3100 abrogated the increase in cell number after CXCL12 stimulation (Fig. 9A)
. AMD3100 treatment alone did not affect cell proliferation compared with the unstimulated control, again suggesting that no autocrine growth stimulatory loops exist, at least in this cell line. These results demonstrate that CXCR4 is necessary and sufficient for CXCL12-induced growth stimulation. AMD3100 also inhibited the invasion of IGROV cells toward CXCL12 (Fig. 9B)
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, Fig. 10a
), including 5 samples from women with a family history of ovarian cancer. There was weak staining of Fallopian tube epithelium and rete ovarii. Seven benign specimens were studied (2 serous, 4 mucinous, and 1 teratoma dermoid), 1 of 2 serous was weakly positive and 1 of 2 was positive, 4 of 4 mucinous cystadenomas and the teratoma were negative. Eight borderline ovarian tumors were studied; the one mucinous borderline was negative, and of the seven serous borderline tumors 3 of 7 were negative, 3 of 7 were weakly positive (Fig. 10b)
, and 1 of 7 was positive.
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, Fig. 10, c and d
). Both endometrioid ovarian cancers were positive. Finally of the five clear cell cancers, 3 of 5 were positive and 2 of 5 were negative. It may be significant that, in keeping with the results on invasion in vitro, enhanced staining was observed in epithelial cells lining cystic spaces or in areas of invasion (Fig. 10c)
and in more pleomorphic neoplastic cells. There was also a trend toward stronger expression in the higher grade tumors. Another consistent observation was increased staining for CXCL12 in tumor-associated myofibroblasts in areas of desmoplasia (Fig. 10d)
. Fibroblast or stromal cell staining was not seen in normal ovaries. These immunohistochemistry data suggest that CXCL12 could be acting locally within the ovarian solid tumor microenvironment, especially in papillary serous carcinomas and that expression of CXCL12 may increase with malignant progression.
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DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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We initially suggested that CXCR4/CXCL12 might be involved in peritoneal spread because we detected high concentrations of CXCL12 in ascitic fluid. However, the data in this paper suggest that this chemokine might have other roles in the solid tumor microenvironment, stimulating DNA synthesis and inflammatory cytokine production as well as invasion through Matrigel. These effects may be mediated by well-defined MAPK and PI3K signaling pathways. CXCL12 may not only stimulate a subset of CXCR4-expressing tumors cells to migrate but may act in an autocrine or paracrine manner to permit tumor cells to grow in suboptimal conditions.
These actions of CXCL12 were not exclusive to IGROV cells. CXCL12 stimulated cell proliferation in another CXCR4-expressing ovarian cancer cell line CAOV-3, with a 2–3-fold increase in cell numbers after 6 days. CXCL12 also stimulated biphasic activation of the MAPK pathway in CAOV-3 cells, their invasion through Matrigel and induced TNF- mRNA in CAOV-3 cells and primary tumor cells.
In our previous study, we detected CXCL12 in 63 ovarian cancer ascitic fluid samples (range, 0.61–9.33 ng/ml; median, 6.02 ng/ml; Ref. 10 ). The concentration of CXCL12 that was active on ovarian cancer cell lines in vitro was generally one log higher than this, but it is entirely possible that local concentrations of the chemokine are much higher in vivo but we have no way of measuring this. Indeed, immunohistochemistry showed very strong staining for CXCL12. It would also be interesting to know if other cytokines/growth factors present in the solid ovarian tumors and ascites synergize with the action of CXCL12 on the tumor cells. In this respect, we note that CXCL12 was not generally secreted by ovarian cancer cells in vitro but cells in vivo stained strongly for this chemokine.
In IGROV and CAOV-3 cells, CXCL12 stimulation resulted in the phosphorylation of p44/42 MAPK (ERK1/ERK2) and stimulation of IGROV cells also resulted in the phosphorylation of Akt/PKB. Phosphorylation of Akt/PKB was maintained for at least 2 h. Activation of Akt/PKB has been shown to have roles in chemotaxis and survival (22) and is recruited to the leading edge of the cell during neutrophil chemotaxis (28) . In fibroblasts, Akt/PKB can promote survival by blocking caspase 3 activity (29) , whereas in hematopoietic cells, Akt/PKB can promote the induction of Bcl-2 and thereby inhibit apoptosis. Cheng et al. (30) demonstrated that Akt/PKB is overexpressed in a small proportion of solid ovarian tumors. In animal models of prostate cancer, increased Akt/PKB activity has also been shown to contribute to tumor progression by accelerating tumor growth (31) .
In IGROV cells, phosphorylation of p44/42 MAPK was biphasic. There was an initial increase in the ratio of phospho-p44/42 MAPK to p44/42 MAPK after 15 min. This ratio then decreased back to control levels after 30 min before increasing again after 1 h. Recent work by Han et al. (25)
showed biphasic activation of p44/42 MAPK in astrocytes in response to CXCL12. Early activation of p44/42 MAPK was directly attributable to CXCL12 stimulation; late activation was indirectly mediated by CXCL12-induced TNF-. A similar mechanism may be responsible for the biphasic response seen in IGROV cells.
The invasion of IGROV and CAOV-3 cells through Matrigel may be dependent on the presence of MMPs and other matrix-degrading enzymes. In vivo, metastasizing cancer cells must degrade extracellular matrix and cross basement membranes to reach the lymphatic system and other sites of metastasis. IGROV expressed mRNA for MMP-7 and MMP-11, which are secreted enzymes, and MMP-15, which is a transmembrane MMP that can activate pro-MMP-2. IGROV also expressed mRNA for TIMP-1 and TIMP-2, which can inhibit the activity of various MMPs, and uPA (32)
. None of these molecules appeared to be regulated by CXCL12. Therefore, it is probable that IGROV cells produce a variety of matrix-degrading enzymes and that CXCL12 merely gives them a direction in which to move. Invasion was inhibited by Marimastat and also anti-TNF- mAb. Apart from being a broad-spectrum MMP inhibitor, Marimastat is also a TNF converting enzyme inhibitor and as such blocks TNF- release from cells (33)
. The doses of Marimastat used in these experiments inhibited TNF- production by THP-1 cells in our laboratory (27)
. There are some reports that TNF- stimulates cancer cell invasion and modulates adhesion molecule expression and activity (34, 35, 36)
. We previously showed that CXCL12 up-regulated ß1 integrin on IGROV and CAOV-3 cells (10)
. The role of TNF- in this integrin up-regulation is now being investigated.
The induction of TNF- in IGROV and CAOV-3 cells and primary tumor cells after CXCL12 stimulation may have additional roles in the tumor microenvironment. When chronically produced, this cytokine may act as an endogenous tumor promoter, contributing to tissue remodeling and stromal development necessary for tumor growth and spread. TNF- mRNA is abundant in malignant ovarian epithelium, and a series of experiments has implicated TNF- in stromal/tumor communication in ovarian cancer (reviewed in Refs. 2
, 37
). However, apart from TNF- itself, this is the first factor that we have found that induces TNF- in ovarian cancer cells. A pulse of TNF- induced by CXCL12 could therefore stimulate production of other cytokines and proteases in the microenvironment around the tumor cell.
In this paper, we have focused on the action of CXCL12 on the epithelial ovarian tumor cells. However, a recent paper defines a paracrine role for this chemokine in the ovarian cancer microenvironment. In agreement with our data, Zou et al. (19) reported that ovarian epithelial tumor cells express high levels of CXCL12. They demonstrated that this CXCL12 induces DC2 precursor chemotaxis and adhesion/transmigration, up-regulates preDC2 VLA-5, and protects pre-DC2s from tumor macrophage IL-10-induced apoptosis. As these pre-DC2 are poor inducers of T-cell responses, tumor immunity may be modulated/dysregulated by CXCL12.
The biological actions of CXCL12 on ovarian cancer cells were inhibited by the bicyclam inhibitor of CXCR4, AMD3100. AMD3100 is a specific antagonist for CXCR4, blocks HIV infection of T-tropic X4-using virus in vitro and in vivo, and inhibits migration of monocytic cells toward CXCL12 (21 , 38) . AMD3100 has been evaluated in a Phase I clinical trial in healthy volunteers and subsequently in HIV-positive patients (39) . The leukocytosis observed in the Phase I trial of AMD3100 led to the initiation of an additional Phase I study in healthy volunteers in which it was demonstrated that AMD3100 was able to mobilize pluripotent hematopoietic stem cells (40) . IGROV cells grow as i.p. xenografts in nude mice, and AM3100 is active in murine models of HIV/AIDS and rheumatoid arthritis (41 , 42) . Hence, we will be able to carry out preclinical studies of the potential of AMD3100 alone and in combination with biological and chemotherapeutic agents in ovarian cancer.
In conclusion, the selective expression of the chemokine receptor CXCR4 and its ligand CXCL12 by malignant ovarian epithelium has a number of important consequences to tumor development and spread. This chemokine/ligand receptor pair may stimulate directed migration and invasion of tumor cells, as well as promoting their growth and the establishment of a tumor-promoting cytokine network in suboptimal conditions and subverting tumor immunity. CXCR4 or its ligand is a target for therapeutic intervention.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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1 C. J. S. and J. L. W. contributed equally to this paper. 
2 To whom requests for reprints should be addressed, Phone: 44-0-20-7882-6106; Fax: 44-0-20-7882-6110; E-mail: frances.balkwill{at}cancer.org.uk
3 The abbreviations used are: TNF-, tumor necrosis factor ; mAb, monoclonal antibody; MAPK, mitogen-activated protein kinase; RT-PCR, reverse transcription-PCR; MMP, matrix metalloproteinase; TIMP, tissue inhibitors of MMP; uPA, urokinase plasminogen activator; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PKB, protein kinase B; PI3k, phosphatidylinositol 3'-kinase; ERK, extracellular signal-regulated kinase.
Received 4/ 3/02. Accepted 8/16/02.
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REFERENCES |
|---|
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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(SDF-1) to CXCR4 chemokine receptor in normal human megakaryoblasts but not in platelets induces phosphorylation of mitogen-activated protein kinase p42/44 (MAPK), ELK-1 transcription factor and serine’threonine kinase AKT. Eur. J. Haematol., 64: 164-172, 2000.[Medline]
provokes up-regulation of 2ß1 and 5ß1 integrins, and cell migration in OST osteosarcoma cells. Cell Biol. Int., 25: 319-329, 2001.[Medline]
-induced motility and invasion of human osteosarcoma cells: possible involvement of NF-
B activation. Clin. Exp. Metastasis, 18: 121-129, 2000.[Medline]
receptor-deficient mice. J. Immunol., 167: 4686-4692, 2001.This article has been cited by other articles:
|
|
 |
|
  Correlation of CXCL12 Expression and FoxP3+ Cell Infiltration with Human Papillomavirus Infection and Clinicopathological Progression of Cervical Cancer Am. J. Pathol., October 1, 2009; 175(4): 1525 - 1535.
|
|
|
|
 |
|
 Y. Akishima-Fukasawa, Y. Nakanishi, Y. Ino, Y. Moriya, Y. Kanai, and S. Hirohashi Prognostic Significance of CXCL12 Expression in Patients With Colorectal Carcinoma Am J Clin Pathol, August 1, 2009; 132(2): 202 - 210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kwong, H. Kulbe, D. Wong, P. Chakravarty, and F. Balkwill An antagonist of the chemokine receptor CXCR4 induces mitotic catastrophe in ovarian cancer cells Mol. Cancer Ther., July 1, 2009; 8(7): 1893 - 1905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Ma, K. Norsworthy, N. Kundu, W. H. Rodgers, P. A. Gimotty, O. Goloubeva, M. Lipsky, Y. Li, D. Holt, and A. Fulton CXCR3 expression is associated with poor survival in breast cancer and promotes metastasis in a murine model Mol. Cancer Ther., March 1, 2009; 8(3): 490 - 498. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Lee, J. M. Kim, and E. J. Lee Functional expression of CXCR4 in somatotrophs: CXCL12 activates GH gene, GH production and secretion, and cellular proliferation J. Endocrinol., November 1, 2008; 199(2): 191 - 199. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. O. Rehman and C.-Y. Wang SDF-1{alpha} Promotes Invasion of Head and Neck Squamous Cell Carcinoma by Activating NF-{kappa}B J. Biol. Chem., July 18, 2008; 283(29): 19888 - 19894. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hatziapostolou, C. Polytarchou, D. Panutsopulos, L. Covic, and P. N. Tsichlis Proteinase-Activated Receptor-1-Triggered Activation of Tumor Progression Locus-2 Promotes Actin Cytoskeleton Reorganization and Cell Migration Cancer Res., March 15, 2008; 68(6): 1851 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Li, H. Alizadeh, and J. Y. Niederkorn Differential Expression of Chemokine Receptors on Uveal Melanoma Cells and Their Metastases Invest. Ophthalmol. Vis. Sci., February 1, 2008; 49(2): 636 - 643. [Abstract] [Full Text] [PDF]
|
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|
|
B. Schade, S. H.L. Lam, D. Cernea, V. Sanguin-Gendreau, R. D. Cardiff, B. L. Jung, M. Hallett, and W. J. Muller Distinct ErbB-2 Coupled Signaling Pathways Promote Mammary Tumors with Unique Pathologic and Transcriptional Profiles Cancer Res., August 15, 2007; 67(16): 7579 - 7588. [Abstract] [Full Text] [PDF]
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 D. Uchida, T. Onoue, Y. Tomizuka, N. M. Begum, Y. Miwa, H. Yoshida, and M. Sato Involvement of an Autocrine Stromal Cell Derived Factor-1/CXCR4 System on the Distant Metastasis of Human Oral Squamous Cell Carcinoma Mol. Cancer Res., July 1, 2007; 5(7): 685 - 694. [Abstract] [Full Text] [PDF]
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 I. Kryczek, S. Wei, E. Keller, R. Liu, and W. Zou Stroma-derived factor (SDF-1/CXCL12) and human tumor pathogenesis Am J Physiol Cell Physiol, March 1, 2007; 292(3): C987 - C995. [Abstract] [Full Text] [PDF]
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 R. A. Siddiqui, K. A. Harvey, G. P. Zaloga, and W. Stillwell Modulation of Lipid Rafts by {Omega}-3 Fatty Acids in Inflammation and Cancer: Implications for Use of Lipids During Nutrition Support Nutr Clin Pract, February 1, 2007; 22(1): 74 - 88. [Abstract] [Full Text] [PDF]
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A. Sutton, V. Friand, S. Brule-Donneger, T. Chaigneau, M. Ziol, O. Sainte-Catherine, A. Poire, L. Saffar, M. Kraemer, J. Vassy, et al. Stromal Cell-Derived Factor-1/Chemokine (C-X-C Motif) Ligand 12 Stimulates Human Hepatoma Cell Growth, Migration, and Invasion Mol. Cancer Res., January 1, 2007; 5(1): 21 - 33. [Abstract] [Full Text] [PDF]
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G. Castellano, J. F. Reid, P. Alberti, M. L. Carcangiu, A. Tomassetti, and S. Canevari New Potential Ligand-Receptor Signaling Loops in Ovarian Cancer Identified in Multiple Gene Expression Studies Cancer Res., November 15, 2006; 66(22): 10709 - 10719. [Abstract] [Full Text] [PDF]
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 F. Dommange, G. Cartron, C. Espanel, N. Gallay, J. Domenech, L. Benboubker, M. Ohresser, P. Colombat, C. Binet, H. Watier, et al. CXCL12 polymorphism and malignant cell dissemination/tissue infiltration in acute myeloid leukemia FASEB J, September 1, 2006; 20(11): 1913 - 1915. [Abstract] [Full Text] [PDF]
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T. C. Walser, S. Rifat, X. Ma, N. Kundu, C. Ward, O. Goloubeva, M. G. Johnson, J. C. Medina, T. L. Collins, and A. M. Fulton Antagonism of CXCR3 Inhibits Lung Metastasis in a Murine Model of Metastatic Breast Cancer. Cancer Res., August 1, 2006; 66(15): 7701 - 7707. [Abstract] [Full Text] [PDF]
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 A. Ottaiano, R. Franco, A. Aiello Talamanca, G. Liguori, F. Tatangelo, P. Delrio, G. Nasti, E. Barletta, G. Facchini, B. Daniele, et al. Overexpression of Both CXC Chemokine Receptor 4 and Vascular Endothelial Growth Factor Proteins Predicts Early Distant Relapse in Stage II-III Colorectal Cancer Patients. Clin. Cancer Res., May 1, 2006; 12(9): 2795 - 2803. [Abstract] [Full Text] [PDF]
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J. D. Holland, M. Kochetkova, C. Akekawatchai, M. Dottore, A. Lopez, and S. R. McColl Differential functional activation of chemokine receptor CXCR4 is mediated by g proteins in breast cancer cells. Cancer Res., April 15, 2006; 66(8): 4117 - 4124. [Abstract] [Full Text] [PDF]
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S. Scala, P. Giuliano, P. A. Ascierto, C. Ierano, R. Franco, M. Napolitano, A. Ottaiano, M. L. Lombardi, M. Luongo, E. Simeone, et al. Human Melanoma Metastases Express Functional CXCR4 Clin. Cancer Res., April 15, 2006; 12(8): 2427 - 2433. [Abstract] [Full Text] [PDF]
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 T. Kakinuma and S. T. Hwang Chemokines, chemokine receptors, and cancer metastasis J. Leukoc. Biol., April 1, 2006; 79(4): 639 - 651. [Abstract] [Full Text] [PDF]
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S. Ohira, M. Sasaki, K. Harada, Y. Sato, Y. Zen, K. Isse, K. Kozaka, A. Ishikawa, K. Oda, Y. Nimura, et al. Possible Regulation of Migration of Intrahepatic Cholangiocarcinoma Cells by Interaction of CXCR4 Expressed in Carcinoma Cells with Tumor Necrosis Factor-{alpha} and Stromal-Derived Factor-1 Released in Stroma Am. J. Pathol., April 1, 2006; 168(4): 1155 - 1168. [Abstract] [Full Text] [PDF]
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 A. Belmadani, P. B. Tran, D. Ren, and R. J. Miller Chemokines regulate the migration of neural progenitors to sites of neuroinflammation. J. Neurosci., March 22, 2006; 26(12): 3182 - 3191. [Abstract] [Full Text] [PDF]
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 F. Vianello, N. Papeta, T. Chen, P. Kraft, N. White, W. K. Hart, M. F. Kircher, E. Swart, S. Rhee, G. Palu, et al. Murine B16 Melanomas Expressing High Levels of the Chemokine Stromal-Derived Factor-1/CXCL12 Induce Tumor-Specific T Cell Chemorepulsion and Escape from Immune Control. J. Immunol., March 1, 2006; 176(5): 2902 - 2914. [Abstract] [Full Text] [PDF]
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 J. A. Burger and T. J. Kipps CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment Blood, March 1, 2006; 107(5): 1761 - 1767. [Abstract] [Full Text] [PDF]
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K. Yasumoto, K. Koizumi, A. Kawashima, Y. Saitoh, Y. Arita, K. Shinohara, T. Minami, T. Nakayama, H. Sakurai, Y. Takahashi, et al. Role of the CXCL12/CXCR4 Axis in Peritoneal Carcinomatosis of Gastric Cancer Cancer Res., February 15, 2006; 66(4): 2181 - 2187. [Abstract] [Full Text] [PDF]
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 T. Florio, S. Casagrande, F. Diana, A. Bajetto, C. Porcile, G. Zona, S. Thellung, S. Arena, A. Pattarozzi, A. Corsaro, et al. Chemokine Stromal Cell-Derived Factor 1{alpha} Induces Proliferation and Growth Hormone Release in GH4C1 Rat Pituitary Adenoma Cell Line through Multiple Intracellular Signals Mol. Pharmacol., February 1, 2006; 69(2): 539 - 546. [Abstract] [Full Text] [PDF]
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 H. Schabath, S. Runz, S. Joumaa, and P. Altevogt CD24 affects CXCR4 function in pre-B lymphocytes and breast carcinoma cells J. Cell Sci., January 15, 2006; 119(2): 314 - 325. [Abstract] [Full Text] [PDF]
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I. Airoldi, L. Raffaghello, E. Piovan, C. Cocco, B. Carlini, A. Amadori, M. V. Corrias, and V. Pistoia CXCL12 Does Not Attract CXCR4+ Human Metastatic Neuroblastoma Cells: Clinical Implications Clin. Cancer Res., January 1, 2006; 12(1): 77 - 82. [Abstract] [Full Text] [PDF]
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H. Kulbe, T. Hagemann, P. W. Szlosarek, F. R. Balkwill, and J. L. Wilson The Inflammatory Cytokine Tumor Necrosis Factor-{alpha} Regulates Chemokine Receptor Expression on Ovarian Cancer Cells Cancer Res., November 15, 2005; 65(22): 10355 - 10362. [Abstract] [Full Text] [PDF]
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A. Katayama, T. Ogino, N. Bandoh, S. Nonaka, and Y. Harabuchi Expression of CXCR4 and Its Down-Regulation by IFN-{gamma} in Head and Neck Squamous Cell Carcinoma Clin. Cancer Res., April 15, 2005; 11(8): 2937 - 2946. [Abstract] [Full Text] [PDF]
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S.-B. Peng, V. Peek, Y. Zhai, D. C. Paul, Q. Lou, X. Xia, T. Eessalu, W. Kohn, and S. Tang Akt Activation, but not Extracellular Signal-Regulated Kinase Activation, Is Required for SDF-1{alpha}/CXCR4-Mediated Migration of Epitheloid Carcinoma Cells Mol. Cancer Res., April 1, 2005; 3(4): 227 - 236. [Abstract] [Full Text] [PDF]
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C. Laverdiere, B. H. Hoang, R. Yang, R. Sowers, J. Qin, P. A. Meyers, A. G. Huvos, J. H. Healey, and R. Gorlick Messenger RNA Expression Levels of CXCR4 Correlate with Metastatic Behavior and Outcome in Patients with Osteosarcoma Clin. Cancer Res., April 1, 2005; 11(7): 2561 - 2567. [Abstract] [Full Text] [PDF]
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S. Scala, A. Ottaiano, P. A. Ascierto, M. Cavalli, E. Simeone, P. Giuliano, M. Napolitano, R. Franco, G. Botti, and G. Castello Expression of CXCR4 Predicts Poor Prognosis in Patients with Malignant Melanoma Clin. Cancer Res., March 1, 2005; 11(5): 1835 - 1841. [Abstract] [Full Text] [PDF]
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M. C. P. Smith, K. E. Luker, J. R. Garbow, J. L. Prior, E. Jackson, D. Piwnica-Worms, and G. D. Luker CXCR4 Regulates Growth of Both Primary and Metastatic Breast Cancer Cancer Res., December 1, 2004; 64(23): 8604 - 8612. [Abstract] [Full Text] [PDF]
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F. Marchesi, P. Monti, B. E. Leone, A. Zerbi, A. Vecchi, L. Piemonti, A. Mantovani, and P. Allavena Increased Survival, Proliferation, and Migration in Metastatic Human Pancreatic Tumor Cells Expressing Functional CXCR4 Cancer Res., November 15, 2004; 64(22): 8420 - 8427. [Abstract] [Full Text] [PDF]
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C. Murdoch, A. Giannoudis, and C. E. Lewis Mechanisms regulating the recruitment of macrophages into hypoxic areas of tumors and other ischemic tissues Blood, October 15, 2004; 104(8): 2224 - 2234. [Abstract] [Full Text] [PDF]
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G. G. Vaday, S.-B. Hua, D. M. Peehl, M. H. Pauling, Y.-H. Lin, L. Zhu, D. M. Lawrence, H. D. Foda, and S. Zucker CXCR4 and CXCL12 (SDF-1) in Prostate Cancer: Inhibitory Effects of Human Single Chain Fv Antibodies Clin. Cancer Res., August 15, 2004; 10(16): 5630 - 5639. [Abstract] [Full Text] [PDF]
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Y. S. Haviv, W. J. van Houdt, B. Lu, D. T. Curiel, and Z. B. Zhu Transcriptional targeting in renal cancer cell lines via the human CXCR4 promoter Mol. Cancer Ther., June 1, 2004; 3(6): 687 - 691. [Abstract] [Full Text] [PDF]
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R. A. Bartolome, B. G. Galvez, N. Longo, F. Baleux, G. N. P. van Muijen, P. Sanchez-Mateos, A. G. Arroyo, and J. Teixido Stromal Cell-Derived Factor-1{alpha} Promotes Melanoma Cell Invasion across Basement Membranes Involving Stimulation of Membrane-Type 1 Matrix Metalloproteinase and Rho GTPase Activities Cancer Res., April 1, 2004; 64(7): 2534 - 2543. [Abstract] [Full Text] [PDF]
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A. Prasad, A. Z. Fernandis, Y. Rao, and R. K. Ganju Slit Protein-mediated Inhibition of CXCR4-induced Chemotactic and Chemoinvasive Signaling Pathways in Breast Cancer Cells J. Biol. Chem., March 5, 2004; 279(10): 9115 - 9124. [Abstract] [Full Text] [PDF]
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K. Jankowski, M. Kucia, M. Wysoczynski, R. Reca, D. Zhao, E. Trzyna, J. Trent, S. Peiper, M. Zembala, J. Ratajczak, et al. Both Hepatocyte Growth Factor (HGF) and Stromal-Derived Factor-1 Regulate the Metastatic Behavior of Human Rhabdomyosarcoma Cells, But Only HGF Enhances Their Resistance to Radiochemotherapy Cancer Res., November 15, 2003; 63(22): 7926 - 7935. [Abstract] [Full Text] [PDF]
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G. Helbig, K. W. Christopherson II, P. Bhat-Nakshatri, S. Kumar, H. Kishimoto, K. D. Miller, H. E. Broxmeyer, and H. Nakshatri NF-{kappa} B Promotes Breast Cancer Cell Migration and Metastasis by Inducing the Expression of the Chemokine Receptor CXCR4 J. Biol. Chem., June 6, 2003; 278(24): 21631 - 21638. [Abstract] [Full Text] [PDF]
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S. Barbero, R. Bonavia, A. Bajetto, C. Porcile, P. Pirani, J. L. Ravetti, G. L. Zona, R. Spaziante, T. Florio, and G. Schettini Stromal Cell-derived Factor 1{alpha} Stimulates Human Glioblastoma Cell Growth through the Activation of Both Extracellular Signal-regulated Kinases 1/2 and Akt Cancer Res., April 15, 2003; 63(8): 1969 - 1974. [Abstract] [Full Text] [PDF]
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) above control cultures (
), but this increase was inhibited by the anti-CXCR4 neutralizing mAb (
). Values are the mean ± SE of eight determinations. * denotes P < 0.05; ** denotes P < 0.01. One representative experiment of three is shown.
); AMD3100 alone had no effect on cell number (