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Experimental Therapeutics |
College of Pharmacy [Y. M., Y. G., S. S., J. J., X. X., M. G. W., J. L-S. A.], and James Cancer Hospital and Solove Research Institute [M. G. W., J. L-S. A.], The Ohio State University, Columbus, Ohio 43210
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ABSTRACT |
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90% of human tumor cells but seldom in normal somatic cells. This study evaluated the hypothesis that simultaneous shortening of telomeres and inhibition of telomerase results in synergistic and tumor-selective cytotoxicity. In telomerase-positive human pharynx FaDu tumor cells, paclitaxel caused telomere erosion (first detected at 1 h) and apoptosis. Expression of antisense to the RNA component of human telomerase (hTR) inhibited telomerase activity, shortened telomere length, reduced cell growth rate, and resulted in a significant higher sensitivity to paclitaxel. Another telomerase inhibitor, 3'-azido-3'-deoxythymidine (AZT), at a concentration that produced little or no cell detachment or apoptosis, inhibited the telomerase activity and enhanced the paclitaxel-induced cell detachment and apoptosis. AZT also enhanced the activity of paclitaxel in mice bearing well-established s.c. FaDu xenograft tumors (i.e., reduced residual tumor size, enhanced apoptotic cell fraction, and prolonged survival time), without enhancing host toxicity. In contrast, AZT did not enhance the paclitaxel activity in the telomerase-negative osteosarcoma Saos-2 cells nor in FaDu cells where telomerase was already suppressed by antisense hTR, confirming that the AZT effect in parent FaDu cells is mediated through telomerase inhibition. These results demonstrate that combined use of agents targeting both telomere and telomerase yielded synergistic activity selective for tumors that depend on telomerase for telomere maintenance. |
INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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90% of human tumors but seldom in normal somatic cells (6)
. The selective expression of telomerase in tumor cells makes telomerase an attractive therapeutic target. However, telomerase inhibition results in cytotoxicity only after depletion of preexisting telomeres that occur over a significant lag time. For example, telomerase inhibitors resulted in cytotoxicity in HeLa cells after 23–26 cell doublings (7) . The long lag time may also allow for activation of the telomerase-independent ALT4 (8) . The requirement of telomere depletion limits the therapeutic potential of telomerase inhibitors.
We hypothesized that simultaneous shortening of telomeres and inhibition of telomerase may provide synergistic antitumor activity against tumor cells that depend on telomerase for telomere maintenance. This hypothesis was tested using paclitaxel that causes telomere erosion (9)
and two telomerase inhibitors. Antisense to the RNA portion of hTR, which blocks the template for telomere synthesis (7)
, was used as the specific telomerase inhibitor. Because of the difficulty in delivering antisense under in vivo conditions, the merit of the telomere/telomerase-targeting approach in vivo was investigated using a small molecule, AZT. AZT has multiple pharmacological actions, including inhibition of reverse transcriptase, human telomerase reverse transcriptase component, integrase, DNA polymerase 
, and thymidine kinase, and is preferentially incorporated into telomeric DNA and Z-DNA-containing regions of Chinese hamster ovary cells (10, 11, 12, 13, 14, 15)
. To determine the telomerase-directed selectivity in this approach, the effect of telomerase inhibition was compared in telomerase-positive human pharynx cancer FaDu cells and telomerase-negative human osteosarcoma Saos-2 cells that use ALT for telomere maintenance (8)
.
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MATERIALS AND METHODS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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-32P-ATP from ICN (Costa Mesa, CA); and all other culture supplies and Lipofectamine from Life Technologies, Inc. (Grand Island, NY). All other biological reagents, except those noted otherwise, were purchased from Roche (Indianapolis, IN). Stock solutions of paclitaxel were dissolved in 100% ethanol, G418, and IPTG in PBS, and AZT was dissolved in distilled water. Human pharynx cancer FaDu and osteosarcoma Saos-2 cells, purchased from American Type Culture Collection (Manassas, VA), were maintained in minimum essential medium and McCoy’s medium, respectively.
Construction of Recombinant Expression Vector Containing Antisense or Sense hTR.
Total RNA was isolated from FaDu cells and cDNA was synthesized. A 185-bp hTR fragment was obtained using reverse transcriptase-PCR with the primers 5'-CAGCTGACAT TTTTTGTTTGCTCTA-3' and 5'-GGGTTGCGGAGGGTGGGCCT-3'. The 185-bp fragment was isolated using Agarose Gel DNA Extraction Kit and ligated with pGEM-T Easy Vector (Promega, Madison, WI). The recombinant plasmid was digested using NotI, and the resulting 219-bp DNA fragment was subcloned into the NotI site of the expression vector pOPRSVI/MCS (LacSwitch II Inducible Mammalian Expression System; Stratagene, La Jolla, CA). The presence and orientation of the insert in the recombinant plasmid were verified by: (a) digestion with EcoRI and selecting the five clones that contained the insert; (b) digestion with PvuII, which produced fragments of 2856, 1296, 1106, and 608 bp for antisense recombinant plasmids and fragments of 2856, 1481, 1106, and 423 bp for sense recombinant plasmids. The results demonstrated one clone as sense and four clones as antisense orientations; and (c) confirmation of the antisense and sense orientation by sequencing with T7 or T3 promoter primer (fmol DNA Sequencing System; Promega).
Transfection of FaDu Cells with Recombinant Expression Vectors.
FaDu cells were stably transfected using the Lipofectamine method, first with the repressor vector pCMVLac I (LacSwitch II Inducible Mammalian Expression System) and subsequently with the pOPRSVI/MCS-antisense or sense hTR recombinant plasmid DNA. The clones expressing both the Lac repressor and antisense or sense hTR were selected and maintained in 200 µg/ml hygromycin B and 400 µg/ml G418. IPTG (3 mM), which decreases the binding affinity of Lac repressor protein to the operator vector and triggers transcription and expression of the inserted gene, was used to induce the expression of antisense or sense hTR.
Measurement of Telomerase Activity and Telomere Length.
A modified nonradioactive TRAP was used to measure telomerase activity in all experiments, except for the AZT experiments, which used intracellular TRAP (16
, 17)
. Telomere length was measured by three methods. The mean length of terminal restriction fragments in total cells was measured using our recently developed solution hybridization-based method, i.e., TALA (18)
. This method measured both the amount and length of telomere (18)
. In brief, genomic DNA was isolated, and 10 µg of DNA were digested at 37°C overnight with 10 units each of HinfI/CfoI/HeaIII. The oligonucleitide probe (TTAGGG)4 was labeled by -32P-ATP with polynucleotide T4 kinase. Three ng of the probe was added to 2.5 µg of DNA solution. After denaturation at 98°C for 5 min, hybridization was performed at 55°C overnight. The resulting samples were electrophoresed on 0.7% agarose gel. After drying under vacuum without heating, the gel was exposed to phosphorimage screen, and the result was analyzed using the area-under-curve method of the ImageQuant software from Molecular Dynamics (Sunnyvale, CA). The point representing 50% of the area-under-curve was the mean telomere length.
FISH was used to measure the telomere signals in individual cells (18) . Flow-FISH was used to measure the fluorescence signal and the DNA content of a cell and to calculate the average telomere signals normalized to DNA contents in total cells. Flow-FISH was performed as previously described (19) , with the exception that cells were fixed in 10% formaldehyde followed by three washes with PBS containing 0.1% BSA.
In Vitro Drug Activity Evaluation.
Drug treatment was initiated after cells were allowed to attach to the growth surface. AZT treatment was initiated 24 h before paclitaxel treatment to allow for the conversion of AZT to nucleotides. Drug effects were measured as (a) a reduction of the number of cells that remained attached to the growth surface, using the sulforhodamine B assay (20)
, (b) an increase in the fraction of cells that detached from the growth surface, i.e., ratio of (detached cells that were the cells in the supernatant of the culture medium and in two subsequent Versene rinses) to (attached cells that were harvested by trypsinization after Versene rinses), and (c) an increase in the extent of apoptosis as indicated by the release of DNA-histone complex from the nucleus to the cytoplasm (20)
.
The effect of antisense hTR on paclitaxel activity was additionally evaluated using a clonogenic assay as described previously (21) . Briefly, 2.5 x 103 cells were seeded on 6-well plates for 24 h before dosing with different concentrations (0, 0.5, 1.0, 2.5, 5.0, and 10.0 nM) of paclitaxel. After treatment for 96 h, the numbers of colonies with >16 cells, in five randomly selected microscopic fields/well at 25x magnification, were counted.
M-phase cells were identified morphologically after Giemsa staining by the disappearance of nucleus membrane and appearance of chromosomes.
In Vivo Treatment with Paclitaxel and/or AZT.
FaDu cells were implanted s.c. (106 viable cells in 0.1-ml physiological saline) in the right and left flanks of male Balb c/nu/nu mice (6–8 weeks old, National Cancer Institute). Mice were cared for in accordance with institutional guidelines. Drug treatment was initiated 2 weeks after tumor implantation or when tumors in all animals reached a size of >3 mm in diameter. The paclitaxel group received 5 consecutive daily i.v. injections of 10 mg/kg/day via the tail vein; the dosing solution (200 µl) consisted of 1 volume of paclitaxel dissolved in Cremophor/ethanol and 9 volumes of physiological saline. The control group received 5 consecutive daily treatment of 200-µl physiological saline. The AZT group received a 7-day infusion of AZT at a rate of 200 ng/h, delivered by a 1002D minipump (Alzet Corp., Palo Alto, CA) s.c. implanted on the back of the animal and removed at the end of infusion. The combination group received both drugs, with the paclitaxel treatment initiated 1 day after the initiation of AZT treatment. Some animals were euthanized on day 10, and the tumors were removed and processed for histological evaluation. The number of apoptotic cells and nonapoptotic cells in tumors, in four randomly selected microscopic fields at x400 magnification, were determined using image analysis procedures as described previously (22)
. A separate experiment determined the surviving fraction and the survival time where animals were monitored for 100 days or until death or moribundity. Moribundity was reached when the length of the tumor was 
1 cm.
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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40% (Fig. 1A)
. This was achieved at 100-1000 nM paclitaxel. Subsequent studies used 200 nM, which resulted in cell detachment from the growth surface and apoptosis. The fraction of detached cells increased with the duration of paclitaxel treatment from <1% before treatment to 14% at 12 h and 40% at 48 h (Fig. 1B)
. Morphological evaluation indicated that >60% of the attached cells were interphase cells, and >80% of the detached cells were M-phase cells.
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).
Telomere Erosion in FaDu Cells by Paclitaxel.
The TALA results indicate that paclitaxel treatment for up to 48 h did not affect the telomere length in the attached cells but resulted in significant telomere length reduction in detached cells (Fig. 2A
, Table 1
). TALA measures the population-average telomere length of all cells and does not indicate the telomere status in individual cells. Hence, we also used FISH to visualize the telomere status in individual cells and used Flow-FISH to quantify the telomere length in individual cells and to standardize the telomere length by the DNA content. This normalization was necessary because paclitaxel is known to increase the fraction of cells in the G2-M phase with tetraploid DNA.
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. These results are consistent with the TALA results (Table 1)
. No significant differences were observed in the extents of telomere erosion/shortening measured by these two methods (P > 0.14), indicating that these methods gave comparable quantitative measurements.
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. This effect was observed in some but not all chromosomes. After paclitaxel treatment, the fraction of M-phase cells with eroded telomeres increased from 40% at 1 h to 70% at 48 h in the detached cells and from 20% at 1 h to 50% at 48 h in the attached cells (Fig. 2C)
. In comparison, only 10% of the M-phase cells in the untreated controls showed telomere erosion. No significant changes in telomere signals were observed in interphase cells (data not shown), indicating that the telomere erosion effect of paclitaxel is specific to M-phase cells.
Changes in Telomerase Activity after Paclitaxel Treatment.
In FaDu cells, paclitaxel induced the telomerase activity at early time points, reaching a peak level of 150% of the pretreatment level at 12 h. The telomerase activity then returned to the pretreatment level at 24 h and further declined to 60 and 20% of the pretreatment level at 48 and 96 h, respectively (Fig. 3)
. Similar changes were observed in attached and detached cells, with greater changes in the detached cells.
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74%; Fig. 4A
), gradual reduction in telomere length (30–38% after 42 days; Fig. 4B
), and slower growth rate (increase of doubling time from 22 h to 27 h after 19 days of IPTG induction), whereas the four control groups did not show these changes. After IPTG induction for 55–60 days, most antisense-expressing cells began to detach from the flask and showed apoptotic morphology. The remaining cells stopped growing and showed morphological characteristics of cell senescence (23)
, including enlarged and flattened morphology with increased granularity (data not shown). In contrast, none of the four types of controls showed signs of apoptosis or senescence after 100 days.
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5 and 60% cell growth inhibition at 48 h, a slight increase in the detached cell fraction (<7.5% at 24 and 48 h, Fig. 1B
) and no measurable apoptosis at up to 48 h (Fig. 1C)
. Fig. 4A
shows that AZT inhibited telomerase, with 40 and 100% inhibition of telomerase activity at 1 and 10 µM AZT, respectively. Table 2
shows that 48-h treatment with 10 µM AZT did not affect the telomere length of attached cells. The detached fraction was not analyzed because of inadequate cell numbers.
Effect of Telomerase Inhibition on Paclitaxel Activity in FaDu Cells.
Measurements of cytotoxicity by cell number reduction and clonogenic assays show similar results; cotreatment with antisense hTR significantly increased paclitaxel activity (Fig. 5, A and B)
. The IC50 of paclitaxel in antisense hTR-expressing cells (i.e., cells stably transfected with antisense hTR and induced by IPTG treatment) was significantly lower than the values in the control cells that were either not transfected or transfected with sense hTR (P < 0.01; Student’s t test). The telomere signal in antisense-expressing cells was also significantly lower than the signal in control cells (Table 2)
.
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), and accelerated the appearance of apoptosis (12 versus 24 h; Fig. 1C
). The differences between paclitaxel alone and paclitaxel plus AZT were statistically significant (P < 0.05; Student’s t test) for the IC30s (after 48-h treatment), cell detachment (after 12–36-h treatment), and apoptosis (after 12–48-h treatment).
Measurement of the mean telomere length using TALA indicates that addition of AZT to paclitaxel did not significantly enhance the extent of paclitaxel-induced telomere erosion in detached cells (Table 1)
. However, because AZT significantly increased the detached cell fraction induced by paclitaxel (Fig. 1B)
, AZT in effect enhanced the number of cells with shortened telomere. In attached cells, addition of AZT to paclitaxel enhanced the telomere length reduction from 5 to 20%. However, the difference was not statistically significant (P = 0.09).
Lack of Effect of AZT in Telomerase-negative Cells.
AZT did not enhance the paclitaxel effect in the telomerase-negative Saos-2 cells (P = 0.6; Student’s t test), nor in FaDu cells where telomerase activity was already inhibited by expression of antisense hTR (P = 0.37; Fig. 5, C and D
).
Effect of AZT on Antitumor Activity of Paclitaxel in Mice Bearing FaDu Xenograft Tumor.
AZT enhanced the in vivo activity of paclitaxel. Table 3
shows the size and the morphological data of tumors obtained 3 days after drug treatment was ended. The paclitaxel/AZT combination resulted in a decrease in tumor size, whereas animals in untreated control and single agent groups showed up to 4-fold increase in tumor size. The combination group also showed 2–4-fold higher fraction of apoptotic cells in the residual tumors compared with the other groups. Fig. 6
shows the long-term survival data measured up to 100 days after initiation of treatment. The combination group showed a longer survival time and a higher survival rate.
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DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Paclitaxel is one of the most important anticancer drugs developed in the last two decades and has a broad spectrum of activity against multiple human tumors (24)
. Paclitaxel promotes polymerization and stabilization of microtubules, causes blockade of cells in the M phase, and induces apoptosis (25)
. Paclitaxel-induced telomere erosion has been observed in metastatic murine melanoma K1735 cells (9)
and is confirmed in this study. The TALA and Flow-FISH results show that telomere erosion by paclitaxel was readily detected in the detached cells but not in the attached cells (Tables 1
and 2
). This is likely a result of the differences in M phase and interphase cell fractions in the attached and detached cells (i.e., >80% detached cells were M-phase cells and >60% of attached cells were interphase cells), because, as shown by the FISH results, telomere erosion by paclitaxel was specific to M-phase cells.
The mechanism of telomere erosion by paclitaxel is unknown but is not likely attributable to telomerase inhibition for the following reasons. First, telomere erosion by paclitaxel occurred while there was a transient increase in telomerase activity (i.e., at or before 12 h; Fig. 3
). Second, telomerase inhibition by antisense hTR or AZT caused gradual and slow reduction of the telomere length, i.e., 35% over 42 days for antisense hTR (Fig. 4B)
and shortening 15–20 bp per cell doubling for AZT (10)
. In contrast, telomere erosion by paclitaxel was more abrupt and extensive (i.e., 
35% after 1 day).
This study indicates telomere shortening/deletion in M-phase cells as an early effect of paclitaxel treatment, occurring at 1 h. This effect precedes other biochemical events during paclitaxel-induced apoptosis. For example, paclitaxel and other microtubule agents activate c-Jun NH2-terminal kinase and inactivate extracellular signal-regulated kinase at 8–16 h, which is followed by caspase-3 activation at 30–48 h in human epidermal cancer KB-3 cells (26) . In human prostate cancer PC-3, leukemia HL60, and Jurkat T cells, paclitaxel activates caspase-3 at 12 h and causes cleavage of poly(ADP-ribose) polymerase at 24 h (27, 28, 29) . The finding that telomere erosion by paclitaxel occurred before the detection of hallmark biochemical events in apoptosis suggests that the early onset telomere erosion is unlikely to be the result of apoptosis.
The consequence of telomere erosion by paclitaxel is not known. Telomere erosion is associated with apoptosis. For example, apoptosis in human HeLa 293 and MW451 cells induced by hydroxyl radicals was associated with telomere erosion and occurred without caspase activation (30) . Telomere shortening by telomerase inhibition induced apoptosis in several cell types (7 , 31, 32, 33, 34) , whereas elongation of telomere in human fibroblast IDH4 and prostate DU145 cells resulted in higher resistance to apoptosis induced by serum depletion (35) . Furthermore, telomerase inhibitors enhanced the cytotoxicity of paclitaxel, indicating the importance of telomere maintenance (or lack thereof) in the paclitaxel activity. A similar finding has been observed for cisplatin; treatment of cisplatin-resistant human glioblastoma U251-MG cells with an antisense to telomerase reduces the telomerase activity and enhances the cisplatin-induced apoptosis (32) . We speculate that telomere erosion contributes to the antitumor activity of paclitaxel. The significance of the transient telomerase induction by paclitaxel is unclear but may be related to the telomere erosion because up-regulation of telomerase activity is associated with telomere shortening (6 , 36) .
Additional studies are needed to determine the mechanism by which paclitaxel induces telomere erosion, the role of telomere erosion in drug-induced apoptosis, and whether the rapid onset telomere erosion effect is specific to paclitaxel, microtubule-targeting agents, or other drug classes.
An interesting and unexpected finding is the eradication of tumors in 20% (2 of 10) of the mice treated with AZT alone (Fig. 4)
. It is not clear which of the known or other yet-unknown effects of AZT resulted in its antitumor effect in FaDu xenograft tumor. However, this finding is consistent with the antitumor activity of AZT observed in other tumor systems, e.g., AZT inhibited the growth of breast cancer cell lines (11)
and methylnitrosourea-induced rat mammary tumors (37)
.
In conclusion, our results establish that simultaneous targeting of telomeres and telomerase represents a potential strategy to take advantage of the tumor specificity of telomerase for the treatment of tumor cells that depend on telomerase for telomere maintenance. The tumor selectivity of this therapeutic approach is suggested by the finding that AZT enhanced the activity of paclitaxel without enhancing the toxicity. Studies on the effect of simultaneous telomere/telomerase targeting on germ-line and stem cells are needed to confirm the tumor selectivity. Studies to determine whether this approach is generally applicable to telomerase-positive tumor cells are also warranted.
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FOOTNOTES |
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1 This study was supported, in part, by National Cancer Institute, NIH, Department of Health and Human Services Research Grant R01CA77091. 
3 To whom requests for reprints should be addressed, at 500 West 12th Avenue, Columbus, OH 43210. Phone: (614) 292-4244; Fax: (614) 688-3223; E-mail: au.1{at}osu.edu
4 The abbreviations used are: ALT, alternative telomere lengthening; AZT, 3'-azido-3'-deoxythymidine; FISH, fluorescence in situ hybridization; Flow-FISH, quantification of fluorescence signal from FISH using flow cytometry; hTR, human telomerase RNA; IPTG, isopropyl-1-thio-ß-D-galactopyranoside; TALA, telomere amount and length assay; TRAP, telomeric repeat amplification protocol.
Received 5/14/02. Accepted 12/ 2/02.
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, control with no drug treatment; 
, AZT alone; 
, paclitaxel alone; 
, paclitaxel plus 1 µM AZT; 
, paclitaxel plus 10 µM AZT. A, reduction of number of FaDu cells attached to growth surface because of treatment, measured by the sulforhodamine B assay. Results are expressed as percentage of controls. For experiments using only paclitaxel, the controls were without drug treatment. For the experiments using combinations of paclitaxel and AZT, because AZT alone reduced the cell number by 
-DNA, cleaved with EcoRI and HindIII). Horizontal white bars depict the locations of the mean telomere length. The mean telomere lengths were 2.9, 2.8, 2.8, 1.9, 1.9, 2.7, 2.6, 1.7, 1.6, 2.7, 2.2, 1.6, 1.6, and 2.7 Kb for Lanes 1 through 14.
), and 1.31 ± 0.03 nM in IPTG + antisense hTR cells (
2 test).