| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
Mayo Clinic School of Medicine, Rochester, Minnesota
 |
ABSTRACT |
|---|
 Top ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
|
INTRODUCTION |
|---|
|
TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
20% of all hepatobiliary malignancies (1
, 2)
. The incidence of this deadly neoplasm is increasing in Western countries (1, 2, 3, 4, 5)
. Surgical resection and liver transplantation are potentially curative therapies. Unfortunately, more than two-thirds of cholangiocarcinomas are too advanced at the time of presentation to benefit from these surgical approaches. Moreover, recurrence after surgery is common and problematic. Currently available chemotherapeutic agents and radiation therapy are ineffective at improving survival (2)
. Accordingly, there is a need for improved therapies for this neoplasm. When drugs or cytokines are used for the treatment of advanced cancers, selective induction of cancer cell apoptosis is a desirable outcome (6, 7, 8) . The cytokine tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is an attractive agent for achieving this goal (9) . This death ligand selectively induces apoptosis in many transformed and malignant cells (10, 11, 12) without apparent toxicity to normal tissues in the rodent, baboon, and chimpanzee (13, 14, 15) . Although one study has indicated that TRAIL might be toxic to human hepatocytes, this toxicity appears to reflect the formulation of cytokine used and to be circumvented with different TRAIL preparations (14 , 16, 17, 18, 19) . The potential importance of the TRAIL pathway for the treatment of cancers is highlighted by the recent introduction of TRAIL receptor (TRAIL-R) agonistic antibodies into human Phase I trials (20) .
One previous study has suggested that cholangiocarcinomas might be susceptible to TRAIL-induced apoptosis (12) . In that report, however, several cholangiocarcinoma cell lines were resistant to TRAIL (12) . TRAIL resistance is common in other cancers and has been attributed to the loss of TRAIL-Rs, up-regulation of TRAIL decoy receptors (DcRs), enhanced expression of cellular FLICE-inhibitory protein [cFLIP; an endogenous inhibitor of death receptor (DR) signaling], or alterations in expression of the Bcl-2 family of proteins (15 , 16) . For example, absence of the proapoptotic proteins Bak and Bax as well as enhanced expression of the antiapoptotic proteins Bcl-2 and Bcl-xL has been shown to convey TRAIL resistance (21, 22, 23, 24, 25) . Cholangiocarcinomas, however, do not manifest changes in Bax, Bak, Bcl-2, or Bcl-XL but, instead, frequently overexpress the antiapoptotic Bcl-2 family member Mcl-1 (26 , 27) . The potential ability of Mcl-1 to inhibit TRAIL-mediated apoptosis has not been previously assessed but may be critical if TRAIL is to be useful for the treatment of cholangiocarcinoma.
The objective of this study was to determine whether Mcl-1 mediates TRAIL resistance in cholangiocarcinoma cells. To address this question, we used TRAIL-resistant cholangiocarcinoma cell lines and small-interfering-RNA (siRNA) technology to attenuate Mcl-1 expression (28, 29, 30) . Results of this analysis not only suggest that selective Mcl-1 down-regulation sensitizes cholangiocarcinoma cell lines to TRAIL-mediated apoptosis, but also identify two approaches for overcoming this Mcl-1-mediated TRAIL resistance.
|
MATERIALS AND METHODS |
|---|
|
TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
Cell Culture.
The human cholangiocarcinoma cell lines KMCH, KMBC, and Witt were grown in DMEM supplemented with 10% fetal bovine serum, 10% bovine calf serum, 100,000 units/liter penicillin, 100 mg/liter streptomycin, 100 mg/liter gentamicin as described previously (31
, 32)
. The nonmalignant human cholangiocyte cell line H69 (33)
was cultured in the same medium.
Cell Treatment and Quantitation of Apoptosis.
FLAG epitope-tagged human recombinant TRAIL (amino acids 95–281) was prepared as described previously (34
, 35)
. Cells were incubated with the FLAG-TRAIL in the presence of anti-FLAG M2 monoclonal antibody (2 µg/ml). Flavopiridol was dissolved in DMSO and was diluted more than 1000-fold into culture medium before treatment. Apoptosis was quantitated by assessing the characteristic nuclear changes of apoptosis (i.e., chromatin condensation and nuclear fragmentation) using the nuclear binding dye 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) and fluorescence microscopy (36)
.
siRNA Treatment.
A specific double-stranded 21-nucleotide RNA sequence homologous to the target message was used to silence Mcl-1, Bcl-2, and Bcl-xL expression. siRNAs for human Bcl-2 and Mcl-1 were purchased from Dharmacon (Lafayette, CO.). siRNA for Bcl-xL was designed and synthesized using the software www.ambion.com and Silencer siRNA Construction kit from Ambion (Austin, TX) according to the manufacturer’s instructions. The sequence of the double-stranded RNA used to block Bcl-xL expression in the current experiments is 5'-AAC TGA CTC CAG CTG TAT CCT CCT GTC TC-3' (T7-promoter bold).
Inhibition of protein expression and TRAIL-induced apoptosis was assessed after transient transfection of cholangiocarcinoma cells with siRNA. Briefly, cells grown in 12-well dishes were transiently transfected with 20 nM siRNA using 6 µl/ml siPORT Lipid (Ambion Inc.) in a total transfection volume of 0.5 ml of DMEM containing 10% fetal bovine serum. After incubation at 37°C/5% CO2 for 5 h, 1.5 ml of normal growth medium was added. Samples were then prepared and analyzed for apoptosis or by immunoblot as described below (see "Immunoblot Analysis").
Stable Transfection of Mcl-1 Hairpin RNA Expression Plasmid.
The pSSH1 plasmid containing the human H1 RNA promoter for the expression of small hairpin RNA (shRNA) was a gift from Dr. D. Billadeau (Mayo Clinic, Rochester, MN). Double-stranded DNA template (5'-CCC CGG GAC TGG CTA GTT AAA CTT CAA GAG AGT TTA ACT AGC CAG TCC CGT TTT TGG AAA-3') was inserted into the pSSH1 plasmid after the H1 RNA promoter (pSSH1-Mcl-1). The DNA insert contains sense and antisense sequences (bold type) of the Mcl-1 mRNA and a 9-nucleotide linker sequence, yielding transcription of shRNA against Mcl-1. The plasmid was subjected to nucleotide sequencing for confirmation.
Transfection with the pSSH1-Mcl-1 plasmid was performed using a standard lipofection method. Stably transfected KMCH clones were selected in medium containing 1200 µg/ml G418. Individual colonies were subcloned and screened for Mcl-1 protein expression by immunoblot analysis. Established clones were grown in DMEM supplemented with 10% fetal bovine serum, 10% bovine calf serum, 100,000 units/liter penicillin, 100 mg/liter streptomycin, 100 mg/liter gentamicin, and 200 mg/liter G418.
Immunoblot Analysis.
Cells were lysed by incubation on ice for 30 min in lysis buffer containing 20 mM Tris-HCl (pH 7.5), 1% Triton X-100, 150 mM NaCl, 10% glycerol, 1 mM Na3VO4, 50 mM NaF, 100 mM phenylmethylsulfonyl fluoride, and a commercial protease inhibitor mixture (Complete Protease Inhibitor Cocktail; Boehringer-Mannheim Biochemica, Mannheim, Germany). After insoluble material was pelleted by centrifugation at 14,000 x g for 15 min at 4°C, the supernatants were collected. Samples were resolved by 12.5% SDS-PAGE, transferred to nitrocellulose membrane, and blotted with primary antibodies at a dilution of 1:1000. Peroxidase-conjugated secondary antibodies (Biosource International, Camarillo, CA) were incubated at a dilution of 1:2000 to 1:10000. Bound antibody was visualized using chemiluminescent substrate (ECL; Amersham, Arlington Heights, IL) and was exposed to Kodak X-OMAT film.
Mitochondrial Membrane Potential.
The mitochondrial membrane potential was monitored in single-cultured KMCH cells using the mitochondrial membrane potential-sensitive dye tetramethylrhodamine ethyl ester as described previously (37)
. Briefly, cells were preincubated in Krebs-Ringer-HEPES buffer containing tetramethylrhodamine ethyl ester (1 µM) for 15 min at 37°C and then were incubated in the presence of 50 nM tetramethylrhodamine ethyl ester throughout the experiment. Cellular fluorescence was visualized using multiparameter digitized video microscopy system (excitation 530 nm; emission 580 nm) and was quantitated using the Metafluor quantitative fluorescence software (Universal Imaging Corp., West Chester, PA) as described previously (38
, 39)
.
Immunocytochemistry.
KMCH cells were grown on coverslips and were fixed with PBS containing 3% paraformaldehyde for 20 min at 37°C. After three washes with PBS, the cells were permeabilized in PBS containing 0.0125% 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfate (CHAPS), washed again in PBS three times for 3 min, and then were blocked for 60 min at 37°C in blocking buffer containing 5% goat serum, 5% glycerol, 0.04% sodium azide in PBS (pH 7.2). The cells were then incubated with anti-active Bax antibody diluted 1:200 in blocking buffer at room temperature for 16 h, washed three times in PBS for 10 min, incubated with tetramethylrhodamine-conjugated goat-antimouse IgG at a concentration of 4 µg/ml for 45 min at 37°C, washed again in PBS three times for 3 min, rinsed in H2O, and mounted using Prolong Antifade kit (Molecular Probes) before examination by fluorescence microscopy.
Subcellular Fractionation.
Cytosolic extracts for cytochrome c immunoblot assay were obtained as described by Leist et al. (40)
. Briefly, at the desired time points, the culture medium was exchanged with ice-cold permeabilization buffer [210 mM D-mannitol, 70 mM sucrose, 10 mM HEPES, 5 mM succinate, 0.2 mM EGTA, 0.15% BSA, 80 µg/ml digitonin (pH 7.2)]. After a 5-min incubation at 4°C, cells were centrifuged for 10 min at 13,000 x g. Supernatants representing the cytosolic extracts were used for immunoblot analysis.
Caspase Activation.
Cells were cultured on 35-mm glass-bottomed microwell dishes (Mattek Corp., Ashland, MA) at a density of 1000 cells/dish. Total cellular caspase activation was assessed in single cells using the fluorophore-tagged pancaspase inhibitor N-(N
-carboxyfluoresceinvalinylalanyl)aspartic acid fluoromethylketone [(FAM-VAD-fmk) CaspaTag; Intergen Co., Purchase, NY]. Briefly, the commercially supplied lyophilized FAM-VAD-FMK was reconstituted into a 150x stock with DMSO and was further diluted into a 30x working solution with PBS. Before the experiment, 10 µl of this solution was added to 290 µl of PBS for each dish. The DMEM was gently aspirated and was replaced with 300 µl of the fluorophore-containing buffer. Cells were incubated at 37°C for 1 h, washed twice with the supplied working solution wash buffer, and mounted on the microscope stage of an inverted fluorescent microscope (Axiovert 35M; Carl Zeiss, Inc., Thornwood, NY). Excitation light provided by a 100-W mercury vapor lamp was passed through an interference and neutral-density filter wheel assembly (Eastern Microscope, Research Triangle Park, NC) to select wavelength and intensity under computer control. Fluorescence was visualized using excitation and emission wavelengths of 490 nm and 520 nm, respectively. Digital images were captured using a cooled charged coupling device camera (CCD camera, KAF-1400; Photometrics, Tucson, AZ) and were analyzed and quantitated using fluorescence imaging software (Metafluor Imaging System; Universal Imaging Corp.). Fluorescence intensity of individual cells was quantitated; and the average fluorescence intensity of 100 cells per experimental group was determined.
Statistical Analysis.
All of the data represent at least three independent experiments and are expressed as the means ± SD unless otherwise indicated. Differences between groups were compared using ANOVA and a post hoc Bonferroni test was used to correct for multiple comparisons.
|
RESULTS |
|---|
|
TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
. TRAIL induced minimal apoptosis, 2.5-fold above control values, in the transformed but nonmalignant cell line H69 (9 ± 2% versus 4 ± 1%; P < 0.05). In contrast, apoptosis above control rates was not observed for any of the three human cholangiocarcinoma cell lines. Thus, these three cholangiocarcinoma cell lines are resistant to TRAIL-mediated apoptosis.
|
. The DcRs DcR1 and DcR2 were also detected in nonmalignant cells and cholangiocarcinoma cells, albeit with a decreased expression of DcR1 in Witt cells and an increased expression in KMBC and KMCH cells. Thus, as has been reported in other cell lines (15
, 16
, 41)
, there was no consistent correlation between DcR expression and resistance to TRAIL cytotoxicity. Furthermore, TRAIL insensitivity could not be explained by a failure to express cognate TRAIL DRs.
|
. There was a slight elevation in expression of cFLIPLong in Witt cells and of cFLIPShort in KMBC cells, but in the third cholangiocarcinoma cell line KMCH, both forms of cFLIP were only weakly expressed. Therefore, TRAIL resistance across cell lines also could not be explained by a uniform elevation of cFLIP.
Mcl-1 Expression Is Enhanced in Cholangiocarcinoma Cell Lines.
In some model systems, TRAIL resistance has been ascribed to loss of proapoptotic and/or enhanced expression of antiapoptotic members of the Bcl-2 family. Therefore, expression of these proteins was analyzed by immunoblot analysis (Fig. 1C)
. The cell lines appeared to express comparable amounts of the antiapoptotic proteins Bax and Bak. The antiapoptotic protein Bcl-2 was expressed in the nonmalignant cell line H69 and in KMCH cells; however, it could not be identified in the two other cholangiocarcinoma cell lines Witt and KMBC. Bcl-xL was present in H69, Witt, and KMBC cells; but almost no Bcl-xL was found in KMCH cells. Mcl-1 was expressed in H69 cells; and its expression was even higher in all three cholangiocarcinoma cell lines. These data raised the possibility that Mcl-1 may render cholangiocarcinoma cell lines resistant to TRAIL-induced cell death.
Mcl-1-siRNA, but Not Bcl-2- or Bcl-xL-siRNA, Sensitizes Cholangiocarcinoma Cells to TRAIL-Induced Apoptosis.
To further evaluate the ability of the three antiapoptotic Bcl-2 family members to inhibit TRAIL-induced apoptosis, siRNA technology was used. Transient transfection with specific siRNAs for Bcl-2, Bcl-xL, and Mcl-1 demonstrated their ability to reduce expression of the corresponding polypeptides (data not shown). In all three cholangiocarcinoma cell lines, the Bcl-2- and Bcl-xL siRNAs failed to significantly increase the sensitivity of the cells to TRAIL (Fig. 2)
. In contrast, Mcl-1 down-regulation significantly enhanced TRAILinduced apoptosis, resulting in apoptosis of 30–50% of KMBC and KMCH cells (Fig. 2, A and B)
and almost 70% of Witt cells (Fig. 2C)
. Taken together, these data strongly suggest that Mcl-1, but not other members of the Bcl-2-family, protect cholangiocarcinoma cells from TRAIL-induced apoptosis.
|
. In addition, the expression of TRAIL death and DcRs as well as cFLIPLong and cFLIPShort was unaltered (Fig. 3, B and C)
. Subsequent studies were performed to compare the time- and dose-dependence of TRAIL-induced apoptosis in the stably transfected cell line and parental KMCH cells. At all of the concentrations examined, TRAIL-mediated apoptosis was significantly greater in the cells transfected with Mcl-1 shRNA, reaching 50% apoptosis at 50 ng/ml TRAIL and a maximum of more than 80% apoptosis at 400–800 ng/ml TRAIL (Fig. 4A)
. In contrast, the parental line barely exhibited 50% apoptosis at 1600 ng/ml TRAIL, indicating a 32-fold lower sensitivity to TRAIL. Thus, like the transient transfections, cells stably transfected with Mcl-1-siRNA were sensitized to TRAIL even over time (Fig. 4B)
.
|
|
.
|
. After incubation with TRAIL for 6 h, a marked increase in activated Bax immunoreactivity was observed in the Mcl-1-shRNA-transfected cells (Fig. 5, B and C)
but not in the TRAIL-treated parent cell line. Thus, inhibiting Mcl-1 expression appears to facilitate mitochondrial depolarization by permitting the activation of the proapoptotic Bcl-protein Bax at mitochondria.
Bax promotes apoptosis by inducing mitochondrial release of cytochrome c, which in turn facilitates caspase activation via the apoptosome (44)
. To ensure that the observed Bax activation resulted in the postulated downstream changes, cytochrome c release into the cytosol and caspase activation was examined. After incubation with TRAIL, cytochrome c was more readily detectable in cytosol from the cell line stably transfected with Mcl-1 shRNA than in cytosol from parental cells (Fig. 6A)
. TRAIL-induced activation of caspases was likewise facilitated by stable transfection with Mcl-1 shRNA (Fig. 6B)
. Taken together, these data suggest that Mcl-1 protects cholangiocarcinoma cells against TRAIL by inhibiting the mitochondrial pathway of apoptosis.
|
. In contrast, other Bcl-2 family proteins, TRAIL-Rs, and cFLIP are unaltered (Fig. 7)
. As was seen with Mcl-1 siRNA (Fig. 3)
and shRNA (Figs. 4)
, pretreatment with flavopiridol sensitizes the KMCH cells to TRAIL-mediated killing (Fig. 8)
.
|
|
|
DISCUSSION |
|---|
|
TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
The present results demonstrate that cholangiocarcinoma cells express TRAIL-Rs but are nonetheless resistant to TRAIL. These cells also express TRAIL DcRs, which bind TRAIL but are incapable of initiating apoptotic signaling cascades because they lack complete cytoplasmic death domains and do not bind Fas-associated death domain protein (FADD; Refs. 15 , 48 ). When first identified, the TRAIL DcRs were thought to induce TRAIL resistance by sequestering TRAIL and preventing it from binding to its legitimate DRs, TRAIL-R1/DR4 and TRAIL-R2/DR5 (48, 49, 50, 51, 52, 53) . Several subsequent studies, however, failed to find any correlation between DcR expression and TRAIL resistance in various cancer types (15 , 16 , 41) . These results were similar to our present studies in human cholangiocarcinoma cell lines, in which expression of the DcRs was variable among the cell lines. Witt cells, for example, minimally expressed DcR1 as compared with the other cells lines but were equally resistant to TRAIL cytotoxicity.
Additional experiments were performed to assess other potential mechanisms of TRAIL resistance. cFLIP is an antiapoptotic protein that interferes with TRAIL signaling at the level of the death-inducing signaling complex (23 , 54, 55, 56, 57, 58) . Although cFLIP has been implicated in TRAIL resistance, expression of this polypeptide was also variable among the cholangiocarcinoma cell lines. cFLIPShort, which is universally regarded as an antiapoptotic polypeptide, was expressed abundantly only by KMBC cells. These data suggested that resistance to TRAIL was likely mediated downstream of the death-inducing signaling complex.
TRAIL-mediated apoptosis, like Fas-induced death, requires downstream mitochondrial dysfunction to fully engage the death program in many cell types (59 , 60) . This mitochondrial dysfunction, which is characterized by mitochondrial permeabilization, loss of mitochondrial transmembrane potential, and release of cytochrome c into the cytosol (61, 62, 63, 64) , is regulated by pro- and antiapoptotic members of the Bcl-2 family. For example, TRAIL-induced apoptosis in a colon carcinoma-derived cell line is dependent on the proapoptotic protein Bax (21 , 22) . Conversely, antiapoptotic Bcl-2 proteins have been shown to inhibit TRAIL-mediated cell killing (24 , 65) . Results of the present study indicate that the inhibition of TRAIL-mediated apoptosis in cholangiocarcinoma cells occurs at the level of mitochondria and is mediated by the antiapoptotic protein Mcl-1. Several observations support this interpretation. First, of the antiapoptotic Bcl-2 family of proteins, only Mcl-1 is consistently overexpressed in all three cholangiocarcinoma cell lines relative to the monmalignant human cholangiocyte H69 cell line. These data are consistent with immunohistochemical studies suggesting that Mcl-1 is overexpressed in human cholangiocarcinoma (27) . Second, when siRNA technology is used to diminish expression of the antiapoptotic proteins Bcl-2, Bcl-xL, and Mcl-1, only Mcl-1 siRNA sensitizes all of the cholangiocarcinoma cell lines to TRAIL-mediated apoptosis. Finally, in a cell line stably transfected with Mcl-1 shRNA, mitochondrial dysfunction with membrane depolarization and cytochrome c release is readily observed after TRAIL treatment. Taken together, these data strongly implicate Mcl-1-mediated inhibition of mitochondrial cytochrome c release as a mechanism of TRAIL resistance in human cholangiocarcinoma cells.
The present results indicate that Mcl-1 plays a dominant role in TRAIL resistance despite the expression of Bcl-2 in the KMCH cell line. This unexpected observation suggests that Mcl-1 and Bcl-2 may have different functions. Indeed, these observations are reminiscent of studies with Bcl-2 and Bcl-xL transgenic mice, in which differential protective effects are observed depending on the model of injury (65 , 66) . More recently, elimination of Mcl-1 has been shown to be required for the initiation of apoptosis after UV light irradiation despite the expression of Bcl-xL (43) . The elegant studies by Nijhawan et al. (43) suggest that Mcl-1 is upstream of Bax translocation to mitochondria, an essential mechanism for TRAIL-mediated apoptosis in colon carcinoma cell lines (22) . Our studies, demonstrating more efficient Bax activation and translocation to mitochondria in cells transfected with Mcl-1 shRNA, are consistent with and help integrate these observations. In human cholangiocarcinoma cells, Mcl-1 would appear to be upstream of Bax in mediating its antiapoptotic effects. Moreover, once Mcl-1 is eliminated, Bax is more efficiently activated by cytotoxic TRAIL-initiated signaling pathways.
Consistent with these results, we observed that flavopiridol, which reduces Mcl-1 expression, also sensitizes cholangiocarcinoma cells to TRAIL. These results confirm and extend the previous suggestion that flavopiridol plus a TRAIL agonist might be a rational combination for treating other malignancies (67) .
The present study focused on the mechanisms of TRAIL resistance in cholangiocarcinoma cells. TRAIL resistance is common in malignant cell lines; and strategies to overcome this resistance will be necessary to augment the therapeutic efficacy of TRAIL for treatment of human solid tumors. Mechanisms of TRAIL resistance are complex and vary among different cell types. The current observations suggest that Mcl-1 is instrumental in mediating TRAIL resistance in human cholangiocarcinomas, as has been recently observed in epidermal growth factor-stimulated fibroblasts (68) . On the basis of the present results, approaches to inhibit Mcl-1 expression will likely be necessary for successful treatment of human cholangiocarcinomas with TRAIL. Mcl-1 may be down-regulated by siRNA approaches or flavopiridol, as shown in this study, or by inhibiting Mcl-1 induction using selective inhibitors of receptor tyrosine kinases (46 , 69 , 70) . Given the uniformly fatal nature of this cancer, additional preclinical studies and perhaps even pilot clinical studies of these approaches in combination with TRAIL are warranted.
|
FOOTNOTES |
|---|
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Requests for reprints: Gregory J. Gores, Mayo Medical School, Clinic, and Foundation, 200 First Street SW, Rochester, MN 55905. Phone: (507) 284-0686; Fax: (507) 284-0762; E-mail: gores.gregory{at}mayo.edu
Received 9/ 3/03. Revised 2/19/04. Accepted 3/12/04.
|
REFERENCES |
|---|
|
TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
|---|
B and protects against TRAIL-mediated apoptosis, yet retains an incomplete death domain. Immunity, 7: 813-20, 1997.[CrossRef][Medline]
-Ray-induced apoptosis in transgenic mice with proliferative abnormalities in their intestinal epithelium: re-entry of villus enterocytes into the cell cycle does not affect their radioresistance but enhances the radiosensitivity of the crypt by inducing p53. Oncogene, 15: 131-41, 1997.[CrossRef][Medline]
TNF-related apoptosis-inducing ligand in human cancer cell lines. Cancer Res, 63: 621-6, 2003.This article has been cited by other articles:
|
|
 |
|
  J. H. Song, K. Kandasamy, and A. S. Kraft ABT-737 Induces Expression of the Death Receptor 5 and Sensitizes Human Cancer Cells to TRAIL-induced Apoptosis J. Biol. Chem., September 5, 2008; 283(36): 25003 - 25013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Mott, S. F. Bronk, R. A. Mesa, S. H. Kaufmann, and G. J. Gores BH3-only protein mimetic obatoclax sensitizes cholangiocarcinoma cells to Apo2L/TRAIL-induced apoptosis Mol. Cancer Ther., August 1, 2008; 7(8): 2339 - 2347. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhou, G.-D. Lu, C.-S. Ong, C.-N. Ong, and H.-M. Shen Andrographolide sensitizes cancer cells to TRAIL-induced apoptosis via p53-mediated death receptor 4 up-regulation Mol. Cancer Ther., July 1, 2008; 7(7): 2170 - 2180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Wang, W. Chen, W. Zeng, L. Bai, Y. Tesfaigzi, S. A. Belinsky, and Y. Lin Akt-mediated eminent expression of c-FLIP and Mcl-1 confers acquired resistance to TRAIL-induced cytotoxicity to lung cancer cells Mol. Cancer Ther., May 1, 2008; 7(5): 1156 - 1163. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. S. Prabhakar, N. Mulherkar, and K. V. Prasad Role of IG20 Splice Variants in TRAIL Resistance Clin. Cancer Res., January 15, 2008; 14(2): 347 - 351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chetoui, K. Sylla, J.-V. Gagnon-Houde, C. Alcaide-Loridan, D. Charron, R. Al-Daccak, and F. Aoudjit Down-Regulation of Mcl-1 by Small Interfering RNA Sensitizes Resistant Melanoma Cells to Fas-Mediated Apoptosis Mol. Cancer Res., January 1, 2008; 6(1): 42 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Meslin, A. Hamai, P. Gao, A. Jalil, N. Cahuzac, S. Chouaib, and M. Mehrpour Silencing of Prion Protein Sensitizes Breast Adriamycin-Resistant Carcinoma Cells to TRAIL-Mediated Cell Death Cancer Res., November 15, 2007; 67(22): 10910 - 10919. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. W. Meng, S.-H. Lee, H. Dai, D. Loegering, C. Yu, K. Flatten, P. Schneider, N. T. Dai, S. K. Kumar, B. D. Smith, et al. MCL-1 as a Buffer for Proapoptotic BCL-2 Family Members during TRAIL-induced Apoptosis: A MECHANISTIC BASIS FOR SORAFENIB (BAY 43-9006)-INDUCED TRAIL SENSITIZATION J. Biol. Chem., October 12, 2007; 282(41): 29831 - 29846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. W. Werneburg, M. E. Guicciardi, S. F. Bronk, S. H. Kaufmann, and G. J. Gores Tumor Necrosis Factor-related Apoptosis-inducing Ligand Activates a Lysosomal Pathway of Apoptosis That Is Regulated by Bcl-2 Proteins J. Biol. Chem., September 28, 2007; 282(39): 28960 - 28970. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Kobayashi, S.-H. Lee, X. W. Meng, J. L. Mott, S. F. Bronk, N. W. Werneburg, R. W. Craig, S. H. Kaufmann, and G. J. Gores Serine 64 Phosphorylation Enhances the Antiapoptotic Function of Mcl-1 J. Biol. Chem., June 22, 2007; 282(25): 18407 - 18417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-R. A. Hussain, C. M. Cheney, A. J. Johnson, T. S. Lin, M. R. Grever, M. A. Caligiuri, D. M. Lucas, and J. C. Byrd Mcl-1 Is a Relevant Therapeutic Target in Acute and Chronic Lymphoid Malignancies: Down-Regulation Enhances Rituximab-Mediated Apoptosis and Complement-Dependent Cytotoxicity Clin. Cancer Res., April 1, 2007; 13(7): 2144 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Chen, Y. Dai, H. Harada, P. Dent, and S. Grant Mcl-1 Down-regulation Potentiates ABT-737 Lethality by Cooperatively Inducing Bak Activation and Bax Translocation Cancer Res., January 15, 2007; 67(2): 782 - 791. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, J. Xu, N. Jhala, P. Pawar, Z. B. Zhu, L. Ma, C.-H. Byon, and J. M. McDonald Fas-Mediated Apoptosis in Cholangiocarcinoma Cells Is Enhanced by 3,3'-Diindolylmethane through Inhibition of AKT Signaling and FLICE-Like Inhibitory Protein Am. J. Pathol., November 1, 2006; 169(5): 1833 - 1842. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Jung, J.-W. Park, K. S. Choi, J.-W. Park, H. I. Lee, K.-S. Lee, and T. K. Kwon Curcumin sensitizes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis through CHOP-independent DR5 upregulation Carcinogenesis, October 1, 2006; 27(10): 2008 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Palacios, R. Yerbes, and A. Lopez-Rivas Flavopiridol Induces Cellular FLICE-Inhibitory Protein Degradation by the Proteasome and Promotes TRAIL-Induced Early Signaling and Apoptosis in Breast Tumor Cells. Cancer Res., September 1, 2006; 66(17): 8858 - 8869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. Kern, A. M. Haugg, A. F. Koch, T. Schilling, K. Breuhahn, H. Walczak, B. Fleischer, C. Trautwein, C. Michalski, H. Schulze-Bergkamen, et al. Cyclooxygenase-2 Inhibition Induces Apoptosis Signaling via Death Receptors and Mitochondria in Hepatocellular Carcinoma. Cancer Res., July 15, 2006; 66(14): 7059 - 7066. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Han, L. A. Goldstein, B. R. Gastman, and H. Rabinowich Interrelated Roles for Mcl-1 and BIM in Regulation of TRAIL-mediated Mitochondrial Apoptosis J. Biol. Chem., April 14, 2006; 281(15): 10153 - 10163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ohira, M. Sasaki, K. Harada, Y. Sato, Y. Zen, K. Isse, K. Kozaka, A. Ishikawa, K. Oda, Y. Nimura, et al. Possible Regulation of Migration of Intrahepatic Cholangiocarcinoma Cells by Interaction of CXCR4 Expressed in Carcinoma Cells with Tumor Necrosis Factor-{alpha} and Stromal-Derived Factor-1 Released in Stroma Am. J. Pathol., April 1, 2006; 168(4): 1155 - 1168. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Clohessy, J. Zhuang, J. de Boer, G. Gil-Gomez, and H. J. M. Brady Mcl-1 Interacts with Truncated Bid and Inhibits Its Induction of Cytochrome c Release and Its Role in Receptor-mediated Apoptosis J. Biol. Chem., March 3, 2006; 281(9): 5750 - 5759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ishimura, H. Isomoto, S. F. Bronk, and G. J. Gores Trail induces cell migration and invasion in apoptosis-resistant cholangiocarcinoma cells Am J Physiol Gastrointest Liver Physiol, January 1, 2006; 290(1): G129 - G136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Liu, P. Eksarko, V. Temkin, G. K. Haines III, H. Perlman, A. E. Koch, B. Thimmapaya, and R. M. Pope Mcl-1 Is Essential for the Survival of Synovial Fibroblasts in Rheumatoid Arthritis J. Immunol., December 15, 2005; 175(12): 8337 - 8345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Karp, A. Passaniti, I. Gojo, S. Kaufmann, K. Bible, T. S. Garimella, J. Greer, J. Briel, B. D. Smith, S. D. Gore, et al. Phase I and Pharmacokinetic Study of Flavopiridol followed by 1-{beta}-D-Arabinofuranosylcytosine and Mitoxantrone in Relapsed and Refractory Adult Acute Leukemias Clin. Cancer Res., December 1, 2005; 11(23): 8403 - 8412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Austin and S. J. Cook Increased Expression of Mcl-1 Is Required for Protection against Serum Starvation in Phosphatase and Tensin Homologue on Chromosome 10 Null Mouse Embryonic Fibroblasts, but Repression of Bim Is Favored in Human Glioblastomas J. Biol. Chem., September 30, 2005; 280(39): 33280 - 33288. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Minagawa, E. A. Kruglov, J. A. Dranoff, M. E. Robert, G. J. Gores, and M. H. Nathanson The Anti-apoptotic Protein Mcl-1 Inhibits Mitochondrial Ca2+ Signals J. Biol. Chem., September 30, 2005; 280(39): 33637 - 33644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wirth, F. Kuhnel, B. Fleischmann-Mundt, N. Woller, M. Djojosubroto, K. L. Rudolph, M. Manns, L. Zender, and S. Kubicka Telomerase-Dependent Virotherapy Overcomes Resistance of Hepatocellular Carcinomas against Chemotherapy and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand by Elimination of Mcl-1 Cancer Res., August 15, 2005; 65(16): 7393 - 7402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yasui, T. Hideshima, M. Hamasaki, A. M. Roccaro, N. Shiraishi, S. Kumar, P. Tassone, K. Ishitsuka, N. Raje, Y.-T. Tai, et al. SDX-101, the R-enantiomer of etodolac, induces cytotoxicity, overcomes drug resistance, and enhances the activity of dexamethasone in multiple myeloma Blood, July 15, 2005; 106(2): 706 - 712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Lashinger, K. Zhu, S. A. Williams, M. Shrader, C. P.N. Dinney, and D. J. McConkey Bortezomib Abolishes Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Resistance via a p21-Dependent Mechanism in Human Bladder and Prostate Cancer Cells Cancer Res., June 1, 2005; 65(11): 4902 - 4908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Han, L. A. Goldstein, B. R. Gastman, A. Rabinovitz, and H. Rabinowich Disruption of Mcl-1{middle dot}Bim Complex in Granzyme B-mediated Mitochondrial Apoptosis J. Biol. Chem., April 22, 2005; 280(16): 16383 - 16392. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Weng, Y. Li, D. Xu, Y. Shi, and H. Tang Specific Cleavage of Mcl-1 by Caspase-3 in Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis in Jurkat Leukemia T Cells J. Biol. Chem., March 18, 2005; 280(11): 10491 - 10500. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Papageorgiou, L. Lashinger, R. Millikan, H. B. Grossman, W. Benedict, C. P. N. Dinney, and D. J. McConkey Role of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand in Interferon-Induced Apoptosis in Human Bladder Cancer Cells Cancer Res., December 15, 2004; 64(24): 8973 - 8979. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Cancer Research | Clinical Cancer Research |
| Cancer Epidemiology Biomarkers & Prevention | Molecular Cancer Therapeutics |
| Molecular Cancer Research | Cancer Prevention Research |
| Cancer Prevention Journals Portal | Cancer Reviews Online |
| Annual Meeting Education Book | Meeting Abstracts Online |
