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XBP1 Is Essential for Survival under Hypoxic Conditions and Is Required for Tumor Growth

¹ Department of Radiation Oncology, Stanford University, Stanford, California; ² Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts; ³ Department of Medicine, Harvard Medical School, Boston, Massachusetts; and ⁴ Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto, Japan

	ABSTRACT

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Hypoxia within solid tumors is a major determinant of outcome after anticancer therapy. Analysis of gene expression changes during hypoxia indicated that unfolded protein response genes were one of the most robustly induced groups of genes. In this study, we investigated the hypoxic regulation of X-box binding protein (XBP1), a major transcriptional regulator of the unfolded protein response. Hypoxia induced XBP1 at the transcriptional level and activated splicing of its mRNA, resulting in increased levels of activated XBP1 protein. After exposure to hypoxia, apoptosis increased and clonogenic survival decreased in XBP1-deficient cells. Loss of XBP1 severely inhibited tumor growth due to a reduced capacity for these transplanted tumor cells to survive in a hypoxic microenvironment. Taken together, these studies directly implicate XBP1 as an essential survival factor for hypoxic stress and tumor growth.

	Introduction

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Hypoxia is a physiologically important endoplasmic reticulum (ER) stress common to all solid tumors. Numerous clinical studies have demonstrated that tumor hypoxia predicts for decreased local control, increased distant metastases, and decreased overall survival in a variety of human tumors (1) . Hypoxia selects for tumors with an increased malignant phenotype (2) and increases the metastatic potential of tumor cells (3) . Understanding the role of hypoxia in tumorigenesis and its influence on anticancer therapy is crucial to improving current cancer treatments (4) .

The ER is an extensive intracellular membrane network that extends throughout the cytoplasm and functions primarily to process newly synthesized secretory and transmembrane proteins. Accumulation of unfolded proteins in this compartment causes ER stress and prolonged ER stress ultimately results in cell death. The cellular response to ER stress consists of at least two coordinated pathways: (1) rapid translational arrest mediated by pancreatic ER kinase or PKR-like ER kinase (PERK); and (2) transcriptional activation of unfolded protein response target genes (5 , 6) . In addition to cancer (7) , the unfolded protein response has therapeutic implications in diseases such as diabetes, atherosclerosis, viral infection, conformational diseases, and cerebrovascular disease (8) .

Koumenis et al. (9) previously demonstrated that translational control of protein synthesis during hypoxia occurs through the activation of PERK. These investigators showed that PERK^–/– mouse embryonic fibroblasts where unable to phosphorylate eIF2 and had decreased survival during hypoxia when compared with wild-type mouse embryonic fibroblasts. They concluded that PERK plays an important role in hypoxia-induced translation attenuation, additionally supporting a role for hypoxia as an inducer of ER stress (9) . When the PERK-dependent translational attenuation pathway is activated, activating transcription factor-4 (ATF-4) mRNA is selectively translated, leading to an induction of ATF-4 downstream target genes (10) . Moreover, ATF-4 protein has also been shown to be stabilized in a hypoxia-induced factor 1(HIF-1)-independent manner during anoxia (11) .

Previous studies have demonstrated that during ER stress, an ER transmembrane endoribonuclease (IRE1) oligomerizes, autophosphorylates, and uses its endoribonuclease activity to excise a 26 nucleotide intron from unspliced X-box binding protein 1 (XBP1) mRNA (12) . These events result in the production of a potent XBP1 transcription factor that regulates a distinct set of unfolded protein response target genes (13) .

In this study, we analyzed global changes in gene expression during hypoxia and found that unfolded protein response-related genes, including genes specifically regulated by XBP1, were most robustly induced during severe hypoxia/anoxia. Because of its role as a major transcriptional activator of these genes and its overexpression in some tumor types (14) , we investigated the effect of hypoxia on XBP1 regulation. Most importantly, we present evidence that loss of XBP1 increases the sensitivity of transformed cells to killing by hypoxia and severely impedes tumor growth. Because loss of XBP1 had little effect on the secretion of proangiogenic factors such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor, the mechanism of impaired tumor growth in XBP1-deficient cells is likely not dependent upon angiogenesis and is a direct consequence of impaired survival of XBP1-deficient cells under hypoxic conditions. These studies support the concept of targeting XBP1 as a therapeutic strategy to eliminate hypoxic cells and inhibit tumor growth.

	Materials and Methods

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Cell Culture and Hypoxia Treatments.
HIF-1 wild-type and knockout mouse embryonic fibroblasts, XBP1 wild-type and knockout mouse embryonic fibroblasts, and HT1080 cells (human fibrosarcoma cell line) were maintained in DMEM supplemented with 10% fetal bovine serum at 37°C in a 5% CO₂ incubator. For all hypoxia experiments, cells were treated at 70 to 80% confluency and maintained in an anaerobic chamber (Sheldon Corp., Cornelius, OR) with pO₂ levels < 0.02%.

Gene Expression Studies.
Mouse genome MGU74Av2 GeneChip arrays (Affymetrix, Santa Clara, CA) were used for mRNA expression profiling. The preparation of samples and hybridization were carried out as described by Affymetrix. In brief, total RNA from ras/c-myc–transformed mouse embryonic fibroblasts exposed to 8 hours of hypoxia were prepared using Trizol (Invitrogen, Carlsbad, CA). Biotinylated RNA was then synthesized using the BioArray RNA transcript labeling kit (Enzo, Farmingdale, NY). Arrays were hybridized with biotinylated in vitro transcription products for 16 hours at 45°C according to the manufacturer’s instructions. The Fluidic Station 400 (Affymetrix) was used for washing, and a three-step staining protocol was used to enhance detection of the hybridized biotinylated RNA. Arrays were scanned by the Affymetrix fluorescence reader (Hewlett Packard). The raw image DAT data files were initially processed using Affymetrix GeneChip software (version 5) to create CEL files. Higher level analysis of microarray CEL files was performed using dChip v1.3 (15) Thorough statistical analysis was carried out using the dChip software (15 , 16) .

XBP1 Small Interfering RNA (siRNA) Studies.
XBP1 wild-type mouse embryonic fibroblasts were infected with a retroviral vector (SFG U3neo), containing an XBP1-specific siRNA sequence as described previously (17) . Control cells were infected with the SFG U3neo empty vector alone. After infection, all cells were selected with 0.5 mg/mL G418 for 10 days. Suppression of XBP1 expression was confirmed by standard Northern and Western blotting techniques as described below.

RNA Isolation and Northern Blotting.
Total RNA was isolated with Trizol according to the manufacturer’s protocol (Invitrogen). Total RNA was denatured with glyoxal (Fisher, Pittsburgh, PA), size fractioned on 1% agarose/sodium phosphate gels, transferred to nylon membranes (Schleicher & Schuell, Keene, NH) and UV cross-linked. Human GRP78, GRP94, XBP1, and VEGF cDNA probes were obtained from American Type Culture Collection (Manassas, VA). Mouse ERDJ4, Herp, p58^IPK, XBP1, and Glut-1 probes were cloned using specific PCR primers. All probes were verified by sequencing. Probes were labeled with ³²P by random priming (Rediprime; Amersham-Pharmacia, Little Chalfont, United Kingdom). Membranes were hybridized in Churches buffer (2% BSA, 7% SDS, 1 mmol/L EDTA in 0.5 mol/L Tris-HCl (pH 7)] at 65°C overnight. The membranes were washed to a stringency of 2x SSC/0.2% SDS at 65°C, exposed to PhosphoImager film (Amersham-Pharmacia) overnight, and visualized on a Storm 860 PhosphoImager (Molecular Dynamics, Amersham). Ribosomal 28S RNA was used as a loading control.

Western Blotting.
Cell extracts were prepared in 9 mol/L urea, 75 mmol/L Tris-HCl (pH 7.5), and 0.15 mol/L ß-mercaptoethanol (Sigma, St. Louis, MO), sonicated briefly, boiled at 95°C for 5 minutes, and loaded onto SDS-PAGE gels. After electrophoresis, the proteins were transferred onto a nitrocellulose membrane (Bio-Rad, Hercules, CA) and blocked in 5% nonfat milk with 0.1% Tween 20 in Tris-buffered saline for 30 minutes at room temperature. XBP1 protein was detected using an antibody specific to the spliced and unspliced forms as described previously (12) .

Survival and Apoptosis Assay.
Cells previously treated in the hypoxia chamber were plated in tissue culture dishes in triplicate at different densities ranging from 300 to 500,000 cells per dish. Plating efficiency was determined by harvesting untreated cells. After 10 to 14 days, the cells were fixed and stained with a solution of 0.25% crystal violet in ethanol. Surviving fraction was determined by counting the number of colonies with >50 cells. Surviving fraction was normalized by the plating efficiency. The results represent the mean of three independent experiments with the error bars representing ±1SD.

For the apoptosis experiments, 10⁶ cells were plated in 10-cm tissue culture dishes and placed into the hypoxia chamber for 24 and 48 hours. Untreated cells (normoxic) cells were plated at the same density and maintained in a 37°C incubator. After the treatment, cells were trypsinized and stained with terminal deoxynucleotidyl transferase-mediated nick end labeling (TUNEL) antibody according to the manufacturer’s protocol (Roche Diagnostics, Indianapolis, IN). Apoptotic cells were visualized and quantitated by fluorescence activated cell sorting (Becton Dickinson, San Jose, CA). Results represent the mean of four independent experiments with the errors bars representing ±1 SD.

Tumor Growth.
Cells (2 x 10⁶) and (5 x 10⁶) were implanted s.c. into the flanks of SCID mice. Each mouse was implanted with spontaneously transformed mouse embryonic fibroblasts that were either XBP1 wild-type or XBP1 knockout. In separate experiments, SCID mice were also implanted with XBP1 wild-type mouse embryonic fibroblasts and siRNA XBP1 mouse embryonic fibroblasts (ix-XBP1). Tumor volume was calculated according to the following formula: V (mm³) = (L x l x hours x )/6, where L is the length, l is the width, and h the height of the tumor, respectively. The error bars represent ±1 SD of the mean volume. For the in vitro cell proliferation assays, XBP1 wild-type and -deficient cells were plated in equal cell numbers and counted during logarithmic growth phase.

Secretion Assays.
Cells (4 x 10⁵) were seeded in 6-cm dishes and incubated overnight. Fresh media were added, and cells were exposed to 6 and 24 hours of normoxia or hypoxia. VEGF was measured by ELISA according to the manufacturer’s protocol (R&D Systems, Indianapolis, IN). Basic fibroblast growth factor was detected using a mouse monoclonal antibody (Transduction Laboratories, Lexington, KY) at 1:1000 dilution. All data are reported as a fold induction relative to normoxic controls. These experiments represent the pooled results of two independent experiments with the error bars representing 1 SD of the mean.

	Results and Discussion

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Ras and c-myc transformed mouse embryonic fibroblasts were exposed to severe hypoxia/anoxia (0.02% O₂) for a period of 8 hours before total RNA was isolated from both normoxic and hypoxic samples for microarray analysis. This experiment was repeated six times to minimize experimentally introduced variations. Among the 12,000 genes present on the MGU74Av2 GeneChip, 285 were found to be induced by at least 2-fold (2.4%). Of the top 30 most robustly induced genes under severe hypoxia/anoxia, 25% were involved in the unfolded protein response. Fig. 1A is a compilation of known unfolded protein response genes that were induced 2-fold during hypoxia. We confirmed by Northern blotting that many of these unfolded protein response genes were transcriptionally induced under hypoxia (Fig. 1B) .

View larger version (31K): [in this window] [in a new window] [Download PPT slide]   Fig. 1. A, rank list of hypoxia-regulated UPR genes that were induced 2-fold using the mouse 12,000 gene MGU74Av2 GeneChip. These experiments were repeated in six independent experiments to minimize experimental variability. B, Northern blot analysis to confirm the induction of unfolded protein response genes by hypoxia (8 hours).

View larger version (35K): [in this window] [in a new window] [Download PPT slide]   Fig. 2. A, GRP78, GRP94, XBP1, and VEGF mRNA expression increased during hypoxia in HT1080 cells. Treatment with 300 nmol/L thapsigargin (Tg), an inhibitor of the Ca-ATPase, was included as a positive control for UPR induction. B, HIF-1 wild-type and -knockout mouse embryonic fibroblasts (MEFs) demonstrated similar splicing of XBP1 mRNA during hypoxia. C, Spliced XBP1 protein was induced during hypoxia in HT1080 cells.

To determine the role for XBP1 in cell survival during these conditions, we compared the amount of apoptosis in cell cultures of XBP1 wild-type and XBP1-deficient mouse embryonic fibroblasts by TUNEL staining (Fig. 3A) . After 48 hours of hypoxia, 62.7 ± 11.8% of the XBP1-knockout cells stained positive for TUNEL staining, whereas only 38.6 ± 9.3% of the wild-type cells stained positive for TUNEL. Under normoxic conditions, 4.5 ± 3.7% of the XBP1-deficient cells and 7.8 ± 4.0% of the wild-type cells stained positive for TUNEL. These data represent the mean of four independent experiments ±1 SD. These results indicate that XBP1-deficient cells are more sensitive to hypoxia-induced apoptosis than XBP1 wild-type cells and are consistent with published data showing increased apoptosis during ER stress in myeloma cells, expressing either a dominant negative or a siRNA vector to inhibit XBP1 expression (17) .

View larger version (11K): [in this window] [in a new window] [Download PPT slide]   Fig. 3. Comparison of apoptosis after 48 hours of hypoxia between (A) XBP1 wild-type and -knockout MEFs. B, comparison of clonogenic survival in XBP1 wild-type and -knockout MEFs (left panel). Comparison of clonogenic survival in control MEFs (neo-XBP1) and siRNA XBP1-knockdown cells (ix-XBP1; right panel). Far right panel was included to demonstrate that XBP1 expression was reduced in cells stably transfected with XBP1 siRNA construct.

We implanted XBP1 wild-type, XBP1-deficient, or XBP1 siRNA-transfected cells (either 2 x 10⁶ or 5 x 10⁶ cells) into the flanks of SCID mice to determine whether the differences that we found in clonogenic survival during hypoxic conditions in vitro translated into impaired tumor growth in XBP1-deficient cells. In each mouse, we implanted XBP1 wild-type cells in one flank and either XBP1-knockout cells or XBP1 siRNA-transfected cells (ix-XBP1) in the contralateral flank. We performed serial tumor measurements up to 63 days (XBP1-knockout cells; Fig. 4A , left panel) and 49 days (ix-XBP1, Fig. 4A , right panel) after implantation. The XBP1-knockout cells were unable to grow as tumors, whereas the ix-XBP1 cells showed a 4-fold delay in tumor growth rate. We attribute the slower tumor growth rate in the ix-XBP1 cells to incomplete suppression of XBP1 by the siRNA vector. The tumor weights at the time the mice were sacrificed are summarized in Fig. 4B . Mice bearing XBP1 wild-type tumors were also received injections of EF5, a nitroimidazole that forms intracellular adducts under hypoxic conditions (18) . Immunohistochemical staining of these tumor sections with EF5- and XBP1-specific antibodies showed excellent colocalization of XBP1 expression with areas of hypoxia (data not shown).

View larger version (33K): [in this window] [in a new window] [Download PPT slide]   Fig. 4. XBP1 is essential for tumor growth. A, Each SCID mouse was implanted subcutaneously with two tumors consisting of 2 x 106 XBP1 wild-type cells in one flank and either 2 x 106 knockout cells or 2 x 106 ix-XBP1 cells in the contralateral flank. In a separate experiment, 5 x 106 XBP1 wild-type or -knockout cells were implanted into separate mice. The XBP1-knockout cells were not able to grow as tumors, and the XBP1-knockdown cells demonstrated a 4-fold tumor growth delay. B, summary of the tumor volume and weights at the completion of the tumor growth delay experiments. C, comparison of in vitro growth rates between XBP1 wild-type and -deficient cells. D, comparison of secretion of proangiogenic factors VEGF and basic fibroblast growth factor in the XBP1 control and -knockdown cells. Error bars represent 1 SD of the mean from two independent experiments.

Because tumor growth requires cells to survive long enough to form a multicellular mass and secrete proangiogenic growth factors, we investigated whether the differences in tumor growth between XBP1 wild-type and -deficient cells were due to a defect in the secretion of proangiogenic factors such as VEGF or basic fibroblast growth factor. We found that this was not the case as XBP1-deficient cells actually secreted a higher basal level of fibroblast growth factor, whereas VEGF secretion was similar at baseline and in response to severe hypoxia/anoxia in XBP1 wild-type and -deficient cells (Fig. 4D) .

Taken together, these studies strongly support a role for XBP1 in mediating survival during hypoxia and in regulating tumor growth. Before the establishment of an adequate blood supply, tumor cells must be able to survive in a microenvironment that is deficient in oxygen, ATP, and other essential nutrients. Under these conditions, the response to ER stress is critical for cell survival. The unfolded protein response pathway functions independently of HIF-1 and directly links XBP1 with tumorigenesis, leading us to conclude that hypoxic regulation of the unfolded protein response is fundamentally important in the development of tumors. Although the exact mechanism for this observation remains to be elucidated, other components of the unfolded protein response pathway are also likely involved. Furthermore, because XBP1-deficient cells were unable to grow as tumors, it is unclear whether XBP1 is essential for establishment of a tumor, regulation of its subsequent growth, or both processes. Future experiments are directed at defining this mechanism.

	ACKNOWLEDGMENTS

 
We thank Dr. Costas Koumenis for insightful discussions and Erin Christofferson for administrative assistance on this project.

	FOOTNOTES

 
Grant support: Eli Lilly-Damon Runyon Clinical Investigator Award (A. Koong), Basque Country Government Grant BFI01.424 (L. Romero-Ramirez), National Cancer Institute Grant CA67166 (Q-T. Le, N. Denko, A. Giaccia), and NIH Grants AI32412 (L. Glimcher) and 1PO150CA100707 (L. Glimcher, A-H. Lee).

The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Requests for reprints: Albert Koong, Stanford University, Department of Radiation Oncology, 269 Campus Drive, CCSR-1245C, Stanford, CA 94305-5152. Phone: (650) 723-7371; Fax: (650) 723-7382; E-mail: akoong{at}stanford.edu

Received 5/ 7/04. Revised 7/ 7/04. Accepted 7/20/04.

	REFERENCES

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