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1 Genome Damage and Stability Centre, University of Sussex, East Sussex, United Kingdom; 2 Fachrichtung Biophysik, Universität des Saarlandes, Homburg/Saar, Germany; and 3 Department of Biochemistry, Kanazawa Medical University, Ishikawa, Japan
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ABSTRACT |
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 Top ABSTRACT INTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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INTRODUCTION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is distinct from the former phosphatidylinositol 3'-kinase-like kinases because it does not appear to function in signal transduction damage response pathways but rather functions in DNA nonhomologous end joining, a pathway for DSB repair (Ref. 15 ; for a review, see Ref. 16 ). Thus, cell cycle checkpoint arrest and p53 phosphorylation occur normally in DNA-PKcs-deficient cells (17) .
An early response to DSBs is phosphorylation of a variant form of the histone H2A designated H2AX. Phosphorylated H2AX (called 
-H2AX) can be visualized as foci by immunofluorescence using phospho-specific antibodies (18
, 19)
. H2AX foci colocalize with foci of other proteins, including NBS1, 53BP1, MDC1, and BRCA1 (3
, 6
, 18
, 20)
. Although the initial recruitment of these proteins appears to be -H2AX independent, their retention as foci at longer times postirradiation does not occur in cells lacking H2AX, leading to the suggestion that -H2AX plays a critical role in the retention of repair factors at the sites of DSBs (21
, 22)
. Serine 139, which lies within an ATM consensus sequence, is the major H2AX residue phosphorylated in response to DNA damage (19)
. One study examining ATM knockout cell lines concluded that IR-induced -H2AX foci formation is ATM dependent (23)
. We have also observed failure to phosphorylate H2AX in A-T lymphoblastoid cell lines [LCLs (24)
]. Together, these data have led to the widely held belief and logical conclusion that -H2AX formation in response to DSBs is an early step in the ATM-dependent signal transduction process. However, another study reported defective H2AX phosphorylation in the DNA-dependent protein kinase (DNA-PK)-defective tumor line M059J (18)
. Here, we have addressed the roles of ATM, ATR, and DNA-PK in H2AX phosphorylation after exposure to IR. We demonstrate that IR-induced H2AX phosphorylation can be carried out by ATM or DNA-PK in a redundant, overlapping manner.
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MATERIALS AND METHODS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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Treatment with DNA-Damaging Agents.
Irradiation was performed using a 137Cs source (8.5 Gy/min). X-irradiation (95 kV, 25 mA, 
6 Gy/min) was used for the experiments involving DT40 chicken cells. When present, LY294002 (200 µM) was added to the cells 30 min before treatment with the relevant agent. LY294002 was purchased from Sigma-Aldrich (Poole, United Kingdom).
Antibodies.
Anti-p53Ser15 and anti-Rad17Ser645 were purchased from Cell Signaling Technology (Beverely, MA) and New England Biolabs (Hitchin, United Kingdom), respectively. Anti-H2AXSer139 antibodies were obtained from Upstate Technology (Buckingham, United Kingdom). The anti-53BP1 mouse monoclonal antibodies were as described previously (31)
. The anti-MDC1 (NH2-terminal FHA domain) rabbit polyclonal antibodies were kindly provided by Dr. M. Goldberg and Prof. S. Jackson. Antirabbit and antimouse secondary antibodies were purchased from Dako (Glostrup, Denmark).
Immunofluorescence.
Cells were fixed in 3% paraformaldehyde and 2% sucrose PBS for 10 min at room temperature and permeabilized in 20 mM HEPES (pH 7.4), 50 mM NaCl, 3 mM MgCl2, 300 mM sucrose, and 0.5% Triton X-100 (Sigma-Aldrich) for 5 min at 4°C. Thereafter, coverslips were washed in PBS before immunostaining. Primary antibody incubations were performed for 40 min at 37°C at 1:100 dilutions (1:800 for anti--H2AX) in PBS supplemented with 2% bovine serum fraction V albumin (Sigma-Aldrich) and followed by washing in PBS. Incubations with antimouse tetramethylrhodamine isothiocyanate and FITC or with antirabbit FITC secondary antibodies (Sigma-Aldrich) were performed at 37°C at 1:100 dilutions in 2% bovine serum fraction V albumin for 20 min. Nuclei were counterstained with 4',6-diamidino-2-phenylindole (Sigma-Aldrich; data not shown) for 10 min at 4°C. Coverslips were mounted in Vectashield (Vector Laboratories, Peterborough, United Kingdom). Foci were counted using a fluorescence microscope. Foci present in 200 cells were counted in each experiment. The error bars represent the SD of the mean. A minimum of three experiments were carried out where error bars are shown. For experiments involving the DT40 cells, cells in suspension were spotted onto glass slides coated with 0.1% gelatin, fixed, and permeabilized with 100% ice-cold methanol for 30 min and ice-cold acetone for 1 min. Further analysis was as described previously (32)
.
DNA-PK Kinase Assay.
Whole cell extracts were prepared by freezing and thawing as described previously (33)
. Cell extract was mixed with pre-swollen and prewashed DNA-cellulose beads (Sigma; 5 mg/reaction) and incubated for 30 min at 4°C. After two washes in Z'0.05 buffer [25 mM HEPES (pH 7.5), 50 mM KCl, 10 mM MgCl2, 1 mM DTT, 1% NP40, and 20% glycerol], the beads were resuspended in the same buffer and used for the kinase assay. The beads were incubated with 1 µl of p53-derived substrate (Promega) and 0.52 µCi of [-32P]ATP for 15 min at 30°C, and then the reaction was stopped by the addition of 30% acetic acid. Reactions were spotted onto P81 cation exchange paper to bind the peptide substrate, washed three times with 15% acetic acid, and then dried. Radioactivity retained on the filters was quantified by liquid scintillation counting.
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RESULTS |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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-H2AX formation after exposure to 2 Gy was examined in two A-T primary fibroblast cell lines (AT1BR and AT7BI), an immortalized A-T cell line (AT5BIVA), and DNA-PKcs-deficient M059J cells. Although the foci formed with slightly slower kinetics in the three A-T cell lines, by 30 min postirradiation, a similar number of foci were observed in A-T and control cell lines (Fig. 1A)
. M059J (DNA-PKcs–/–) and the parental line, M059K (DNA-PKcs+/+), a glioma cell line, gave a more robust response relative to the primary fibroblast cell lines, but a similar peak of foci was observed in both lines (Fig. 1A)
. The elevated number of foci in these glioma cell lines relative to primary cell lines is most likely explained by the fact that the cells are hyperdiploid (modal chromosome number approximately 110), allowing the formation of more DSBs (34)
. Foci both form and commence disappearing more rapidly in the M059K (DNA-PKcs+/+) cells relative to the fibroblasts. By 1 h postirradiation, the number of foci in M059K cells had already decreased, whereas foci were lost more slowly in DNA-PKcs–/– M059J cells, indicative of their impaired DSB repair (26)
. With the fibroblasts, foci remained at a similar level until around 60 min postirradiation, most likely representing a steady state between continued formation and disappearance due to DSB repair. With prolonged incubation, the number of foci decreased, and this occurred to similar extents in A-T and control cells over a 3-h period examined (data not shown).
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. At higher doses, the response ceased to be linear, probably due to inaccuracy in scoring >60 foci/cell. Taken together, these results show that foci form efficiently in human cells lacking either ATM or DNA-PK.
H2AX Phosphorylation Is Ablated in Human A-T Fibroblasts Treated with a DNA-PK-Inhibiting Drug.
One explanation for these findings is that ATM and DNA-PKcs function redundantly to phosphorylate H2AX. To examine this possibility, we measured -H2AX formation in the presence of the drug LY294002, a specific inhibitor of DNA-PKcs, in cells lacking ATM (35, 36, 37)
. Whereas IR-induced foci form normally in the presence of LY294002 in 1BR3, M059K (DNA-PKcs+/+), and M059J (DNA-PKcs–/–) cells, foci were barely detectable in the two A-T fibroblast cell lines (AT1BR and AT7BI) examined (Fig. 1C)
. To confirm that LY294002 does not affect ATR, we exploited a recent finding that F02-98 cells, which have a mutational change in ATR leading to aberrant splicing and impaired ATR activity, show dramatically reduced UV-induced -H2AX formation, demonstrating that this event is ATR dependent (14)
. LY294002 did not impair UV-induced H2AX phosphorylation in 1BR3 cells, demonstrating that ATR remains active in the presence of the drug (data not shown). To verify that LY294002 does not impair ATM function, we examined the impact of the drug LY294002 on IR-induced p53 and Rad17 phosphorylation by immunofluorescence using phospho-specific antibodies (anti-p53Ser15 and anti-Rad17Ser645) and observed similar levels of phosphorylated p53 and Rad17 in control and drug-treated control cells but impaired phosphorylation in AT1BR (data not shown). Taken together, these findings demonstrate that LY294002 does not impair either ATM or ATR activity. To verify that LY294002 does impair DNA-PK activity, we first carried out a DNA-PK kinase assay in the presence of the drug and observed significant inhibition of DNA-PK activity consistent with previous findings (data not shown; Refs. 36
and 37
). To examine the impact on DNA-PK activity in vivo, we counted the H2AX foci remaining at 2 h postirradiation in wild-type MEFs in the presence of LY294002 (Fig. 1D)
. In a manner similar to the results obtained with DNA-PKcs–/– M059J and DNA-PKcs+/+ M059K cells, we observed that H2AX foci form normally in DNA-PKcs+/+ and DNA-PK–/– MEFS (see also below). However, the H2AX foci are lost more slowly in the DNA-PK–/– MEFs, presumably due to the reduced DSB repair observed in DNA-PK-deficient cells (Fig. 1D
; Ref. 26
). Treatment of wild-type MEFs with LY294002 caused the same reduced rate of loss of H2AX foci as that observed in DNA-PKcs–/– MEFs (Fig. 1D)
.
Finally, to examine the contribution of ATR to IR-induced H2AX phosphorylation, we examined foci formation in F02-98 (ATRm/m) cells in the presence and absence of LY294002. In all cases, a similar level of IR-induced H2AX foci relative to control cells was observed (Fig. 1C)
. Thus, we conclude that ATR does not significantly contribute to IR-induced H2AX foci formation, at least over the time scale examined in these experiments.
Taken together, these findings show that in addition to ATM, another phosphatidylinositol 3'-kinase that is inhibited by LY294002 can phosphorylate H2AX after IR treatment. We show that DNA-PK, but not ATM or ATR, is inhibited by LY294002.
IR-Induced H2AX Phosphorylation Occurs Efficiently in MEFS Lacking ATM and Is Ablated by Drug-Induced Inhibition of DNA-PK.
An earlier study carried out on primary and transformed MEFs reported that H2AX phosphorylation is ATM dependent (23)
. To verify that our findings were not specific to human cells, which have 50-fold higher levels of DNA-PK activity compared with mouse cells, we also examined transformed ATM–/– and DNA-PKcs–/– MEFs for induction of -H2AX foci. Similar to our findings with human cells, we observed slightly slower induction of -H2AX foci in ATM–/– MEFs compared with control and DNA-PKcs–/– MEFs, but by 15 min postirradiation, a similar level of foci was observed (Fig. 2)
. Addition of LY294002 ablated H2AX foci formation in ATM–/– MEFs, identical to the results found with human fibroblasts (Fig. 2)
. Thus, our findings suggest that in rodent cells, as in human cells, DNA-PK has a redundant, overlapping function with ATM in phosphorylating H2AX.
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-H2AX Foci in LCLs.-H2AX foci formation in A-T LCLs (24)
. These experiments were carried out with LCLs that had grown to saturation after several days of growth without medium change. To examine whether this might be a factor influencing the response, we monitored IR-induced -H2AX formation in two A-T LCLs replenished by the addition of fresh medium postirradiation in parallel to cells maintained in growth saturating conditions. No significant IR-induced H2AX phosphorylation was observed in the two A-T LCLs [AO (ATM–/–) and LB541 (ATMm/m)] that were maintained in their original medium postirradiation (Fig. 3A)
. In contrast, efficient -H2AX formation similar to that observed in control cells was observed in both A-T LCLs when the medium was changed immediately after irradiation (Fig. 3A)
. The addition of LY294002 inhibited this H2AX phosphorylation. To examine whether DNA-PK is down-regulated in growth-saturated LCLs, we monitored DNA-PK activity in extracts prepared under both conditions (Fig. 3B)
. Although the level of DNA-PK activity was slightly lower when held under nongrowing conditions, the difference was small and unlikely to explain the dramatic impact on H2AX phosphorylation. Thus, the inability of DNA-PK to phosphorylate H2AX in growth-saturated LCLs cannot be explained simply by down-regulation of DNA-PK levels or activity.
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-H2AX foci formation in primary fibroblasts that were actively growing with fibroblasts maintained in plateau phase for 2 weeks. -H2AX foci formation occurred to a similar extent in all samples (Fig. 3C)
. Taken together, we conclude that ATM and DNA-PK can phosphorylate H2AX in a redundant, overlapping manner in response to DSBs. However, under certain growth conditions (e.g., growth-inhibited LCLs), DNA-PK does not appear to play a major role.
ATM and DNA-PK Contribute to IR-Induced H2AX Phosphorylation in Chicken Cells.
To substantiate that it is DNA-PK rather than another phosphatidylinositol 3'-kinase inhibited by LY294002 that overlaps with ATM in H2AX phosphorylation, we sought to examine cells lacking both DNA-PK and ATM and exploited chicken DT40 cells because double mutant cell lines lacking Ku70 and ATM have been described previously (30)
. Because DNA-PK activation requires Ku binding to DNA ends, Ku70 mutants are devoid of DNA-PK activity. We therefore examined foci formation in ATM–/–, Ku70–/–, and ATM–/–Ku70–/– cells at 2 h posttreatment with 1 and 2 Gy of X-rays in the presence and absence of the drug LY294002 using human anti-phospho-H2AX antibody. By Western blotting, we verified that human anti-H2AX antibodies cross-react with chicken H2AX in a manner inducible by IR (Fig. 4A)
. To allow a quantitative examination of H2AX phosphorylation, we examined foci formation as carried out with human and mouse cells (Fig. 4B)
. All three chicken cell lines had a high background of -H2AX foci relative to that observed with mammalian cells, which we attribute to ATR-dependent events (Fig. 4B)
. After irradiation, there was an increase in the number of cells with >5 foci/cell in the ATM–/– and Ku70–/– single mutant cells that was not observed in the ATM–/– Ku70–/– double mutants. The slightly greater increase in -H2AX foci in the Ku70–/– cells relative to ATM–/– cells possibly reflects the inability of Ku-defective cells to repair their DSBs relative to ATM–/– cells during the 2-h incubation period. Furthermore, LY294002 inhibited the radiation-induced -H2AX foci in ATM–/– cells but not in the cell line lacking Ku, consistent with our conclusion that the impact of LY294002 on -H2AX foci formation is due to its inhibition of DNA-PK. Taken together, we conclude that ATM and DNA-PK jointly contribute to H2AX phosphorylation in vertebrate cells.
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. These findings are consistent with the dependency of Rad17 and p53 phosphorylation on ATM (39, 40, 41)
and demonstrate that whereas DNA-PK can phosphorylate H2AX, it is not able to phosphorylate these other components of the signaling pathway. It is worth noting that previous studies have reported that M059J cells have low ATM activity (42)
. The present results show that the level of ATM in M059J cells is sufficient to efficiently phosphorylate H2AX, Rad17, and p53.
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-H2AX dependent (22)
. To examine the contribution of DNA-PK to this process, we examined the formation of MDC1 and 53BP1 foci at 1 h post-IR in 1BR3, AT1BR, M059J, and M059K cells. In control cells, both 53BP1 and MDC1 changed from diffuse nuclear staining with the occasional (1, 2, 3)
large foci in the absence of irradiation to abundant small nuclear foci after radiation exposure. 53BP1and MDC1 foci formed normally in M059J and AT1BR but were abolished in drug-treated AT1BR (Fig. 6)
. These findings consolidate previous reports that retention of these two proteins as foci post-irradiation is ATM independent (22)
. Our results suggest that this is because there is DNA-PK-dependent H2AX phosphorylation, thereby demonstrating that DNA-PK can contribute to the retention of these complexes at the sites of DSBs in the absence of ATM.
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DISCUSSION |
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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-H2AX foci are required for the stable formation of NBS1, 53BP1, MDC1, and BRCA1 foci at the damage sites, this represents an important step that determines subsequent events in the signal transduction pathway (3
, 6
, 18
, 20, 21, 22)
.
Firstly, we show that inactivation of ATM and a second phosphatidylinositol 3'-kinase that is inhibited by the drug LY294002 is required to ablate IR-induced H2AX phosphorylation. Consistent with reported data that LY294002 is a specific DNA-PK inhibitor, we show that in vivo DNA-PK is inhibited by LY294002, whereas both ATM and ATR remain active. We substantiate the notion that DNA-PK is the inhibited kinase by using the DT40 model system to examine chicken cells lacking both ATM and DNA-PK activity. Thus, we conclude that ATM and DNA-PK function redundantly to phosphor-ylate H2AX in response to DSBs. This overlapping function is observed in human, mouse, and vertebrate cells. Whereas our findings show that each kinase can function in the absence of the other, they do not demonstrate which protein phosphorylates H2AX when both proteins are present. Interestingly, DSBs undergo repair and initiate ATM-dependent phosphorylation events concomitantly, indicating that the DNA-PK complex and ATM are both localized to or activated by the double-stranded DNA ends noncompetitively. The slightly slower kinetics of induction of -H2AX foci in ATM-deficient human and mouse cells indicates that ATM may play the dominant role, at least at early times postirradiation. Additionally, we observed ATM-dependent H2AX phosphorylation in growth-saturated A-T LCLs. However, nonproliferating (plateau phase) A-T human fibroblasts phosphorylate H2AX efficiently, suggesting that this observation is not linked to proliferation per se. DNA-PK was not markedly down-regulated under these conditions, suggesting that there may be factors influencing its access to H2AX that remain to be elucidated. Nonetheless, this was only one of many conditions examined where H2AX phosphorylation was found to be specifically ATM dependent. Thus, we conclude that under most normal conditions (actively growing and plateau phase human fibroblasts, growing MEFs and LCLs, and in chicken cells), the two kinases function redundantly, although there are kinetic and growth conditions where ATM predominates.
Two previous studies have addressed which phosphatidylinositol 3'-kinase-like kinase is responsible for IR-induced H2AX phosphor-ylation (18
, 23)
. Our findings disagree with the study of Burma et al. (23)
, which concluded that ATM is the responsible kinase. It is possible that the growth status of the cells could explain this result. Nonetheless, our findings show clearly that DNA-PK can contribute to H2AX phosphorylation after IR. The other study reported a very high background of -H2AX foci in M059J cells that increased only slightly after IR (18)
. In our experiments using 2 Gy, although the background level of foci was elevated in M059J cells, we observed a substantial increase after IR treatment, and the induced level of phosphorylation was similar to that observed in DNA-PKcs+/+ M059K cells. It has been shown previously that M059J cells carry a mutational change in ATM and have reduced ATM as well as DNA-PKcs activity (42)
. However, here we observed efficient Rad17 and p53 phosphorylation (Fig. 5)
, indicating that ATM is functional and capable of efficiently phosphorylating its substrates. Nonetheless, certain conditions might facilitate exposure of the dual phosphatidylinositol 3'-kinase-like kinase requirement in M059J. Paull et al. (18)
used two-dimensional gel analysis to examine H2AX phosphorylation and observed decreased phosphorylation in M059J cells (18)
. This procedure required high doses of IR, and it is possible that the reduced ATM activity in M059J cells could not fully achieve the extensive phosphorylation required, thereby exposing a role for DNA-PK. Although our results are substantially different, our conclusion is similar: namely that ATM and DNA-PK both contribute to H2AX phosphorylation in response to DSBs. Furthermore, the fact that H2AX phosphorylation is reduced after high radiation doses in M059J cells supports our contention that DNA-PK can contribute to H2AX phosphorylation. We also show that ATR does not contribute to IR-induced H2AX phosphorylation in primary fibroblasts, at least over the time scale examined. It is likely, however, that at longer times postirradiation after replication of IR-induced lesions, a contribution of ATR may occur. Our findings do not eliminate this possibility but do show, importantly, that ATR is not activated by DSBs in G1-phase cells.
Activation of ATM by DSBs results in phosphorylation of substrates further downstream that include Chk2, NBS1, CtIP, BLM, and p53. None of these events are observed in A-T cell lines, demonstrating that DNA-PK does not contribute significantly to this aspect of the checkpoint response. Consistent with this, it has been shown that DNA-PKcs-defective cells show checkpoint arrest and p53 phosphor-ylation (17)
. We show that Rad17, which, like H2AX, is located on the chromatin, is also efficiently phosphorylated in DNA-PK-defective cells but not in A-T cells. The fact that DNA-PK phosphorylates H2AX but not other substrates demonstrates the constraints of phosphorylation events in vivo. More recently, another aspect of the DNA damage response has been described in which the role of ATM appears to be redundant, namely, the recruitment of proteins that form as foci that overlap with -H2AX foci (4
, 6
, 21
, 22)
. The proteins that fall into this class include MCD1, 53BP1, MRE11/RAD50/NBS1, and BRCA1. We show that at 1 h post-IR treatment, MDC1 and 53BP1 foci are not visible when both DNA-PK and ATM activities are inhibited but are visible in cells lacking either DNA-PK or ATM alone. Because this correlates with the abolition of -H2AX foci formation, this is consistent with the model that retention of these proteins as foci is dependent on H2AX phosphorylation but not ATM activity. Interestingly, a recent study has suggested that H2AX is not required for the recruitment of these proteins to the DSB but is required for their retention (22)
. Our findings, therefore, provide evidence that DNA-PK plays a redundant, overlapping role with ATM in the retention of these proteins as foci at the damage site by contributing to H2AX phosphorylation.
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ACKNOWLEDGMENTS |
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FOOTNOTES |
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The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Requests for reprints: Penny A. Jeggo, Genome Damage and Stability Centre, University of Sussex, Falmer, Brighton, East Sussex BN1 9RQ, United Kingdom. Phone: 44-1273-678482; Fax: 44-1273-678121; E-mail: p.a.jeggo{at}sussex.ac.uk
Received 10/12/03. Revised 12/22/03. Accepted 1/26/04.
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TopABSTRACTINTRODUCTIONMATERIALS AND METHODSRESULTSDISCUSSIONREFERENCES |
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C. E. Rube, S. Grudzenski, M. Kuhne, X. Dong, N. Rief, M. Lobrich, and C. Rube DNA Double-Strand Break Repair of Blood Lymphocytes and Normal Tissues Analysed in a Preclinical Mouse Model: Implications for Radiosensitivity Testing Clin. Cancer Res., October 15, 2008; 14(20): 6546 - 6555. [Abstract] [Full Text] [PDF]
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A. Kinner, W. Wu, C. Staudt, and G. Iliakis {gamma}-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin Nucleic Acids Res., October 1, 2008; 36(17): 5678 - 5694. [Abstract] [Full Text] [PDF]
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X. Liu, Y. Shi, R. Guan, C. Donawho, Y. Luo, J. Palma, G.-d. Zhu, E. F. Johnson, L. E. Rodriguez, N. Ghoreishi-Haack, et al. Potentiation of Temozolomide Cytotoxicity by Poly(ADP)Ribose Polymerase Inhibitor ABT-888 Requires a Conversion of Single-Stranded DNA Damages to Double-Stranded DNA Breaks Mol. Cancer Res., October 1, 2008; 6(10): 1621 - 1629. [Abstract] [Full Text] [PDF]
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L. C. Riches, A. M. Lynch, and N. J. Gooderham Early events in the mammalian response to DNA double-strand breaks Mutagenesis, September 1, 2008; 23(5): 331 - 339. [Abstract] [Full Text] [PDF]
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 M. L. Kilkenny, A. S. Dore, S. M. Roe, K. Nestoras, J. C.Y. Ho, F. Z. Watts, and L. H. Pearl Structural and functional analysis of the Crb2-BRCT2 domain reveals distinct roles in checkpoint signaling and DNA damage repair Genes & Dev., August 1, 2008; 22(15): 2034 - 2047. [Abstract] [Full Text] [PDF]
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S. Zha, J. Sekiguchi, J. W. Brush, C. H. Bassing, and F. W. Alt Complementary functions of ATM and H2AX in development and suppression of genomic instability PNAS, July 8, 2008; 105(27): 9302 - 9306. [Abstract] [Full Text] [PDF]
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S. L. Bailey, K. E. Gurley, K. Hoon-Kim, K. S. Kelly-Spratt, and C. J. Kemp Tumor Suppression by p53 in the Absence of Atm Mol. Cancer Res., July 1, 2008; 6(7): 1185 - 1192. [Abstract] [Full Text] [PDF]
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 J. Yan, X.-P. Yang, Y.-S. Kim, and A. M. Jetten RAP80 Responds to DNA Damage Induced by Both Ionizing Radiation and UV Irradiation and Is Phosphorylated at Ser205 Cancer Res., June 1, 2008; 68(11): 4269 - 4276. [Abstract] [Full Text] [PDF]
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 K. Yao, W. Wu, Y. Yu, Q. Zeng, J. He, D. Lu, and K. Wang Effect of Superposed Electromagnetic Noise on DNA Damage of Lens Epithelial Cells Induced by Microwave Radiation Invest. Ophthalmol. Vis. Sci., May 1, 2008; 49(5): 2009 - 2015. [Abstract] [Full Text] [PDF]
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Z. Hu, H. Liu, H. Wang, R. Miao, W. Sun, G. Jin, Y. Wang, H. Ma, L. Jin, Q. Wei, et al. Tagging Single Nucleotide Polymorphisms in Phosphoinositide-3-Kinase-Related Protein Kinase Genes Involved in DNA Damage "Checkpoints" and Lung Cancer Susceptibility Clin. Cancer Res., May 1, 2008; 14(9): 2887 - 2891. [Abstract] [Full Text] [PDF]
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J. Hejna, M. Holtorf, J. Hines, L. Mathewson, A. Hemphill, M. Al-Dhalimy, S. B. Olson, and R. E. Moses Tip60 Is Required for DNA Interstrand Cross-link Repair in the Fanconi Anemia Pathway J. Biol. Chem., April 11, 2008; 283(15): 9844 - 9851. [Abstract] [Full Text] [PDF]
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S. Franco, M. M. Murphy, G. Li, T. Borjeson, C. Boboila, and F. W. Alt DNA-PKcs and Artemis function in the end-joining phase of immunoglobulin heavy chain class switch recombination J. Exp. Med., March 17, 2008; 205(3): 557 - 564. [Abstract] [Full Text] [PDF]
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G. Pennarun, C. Granotier, F. Hoffschir, E. Mandine, D. Biard, L. R. Gauthier, and F. D. Boussin Role of ATM in the telomere response to the G-quadruplex ligand 360A Nucleic Acids Res., March 1, 2008; 36(5): 1741 - 1754. [Abstract] [Full Text] [PDF]
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B. Muller, J. Blackburn, C. Feijoo, X. Zhao, and C. Smythe DNA-activated protein kinase functions in a newly observed S phase checkpoint that links histone mRNA abundance with DNA replication J. Cell Biol., January 28, 2008; 179(7): 1385 - 1398. [Abstract] [Full Text] [PDF]
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S. S. Palii, B. O. Van Emburgh, U. T. Sankpal, K. D. Brown, and K. D. Robertson DNA Methylation Inhibitor 5-Aza-2'-Deoxycytidine Induces Reversible Genome-Wide DNA Damage That Is Distinctly Influenced by DNA Methyltransferases 1 and 3B Mol. Cell. Biol., January 15, 2008; 28(2): 752 - 771. [Abstract] [Full Text] [PDF]
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S. J.H. Arlander, B. T. Greene, C. L. Innes, and R. S. Paules DNA Protein Kinase Dependent G2 Checkpoint Revealed following Knockdown of Ataxia-Telangiectasia Mutated in Human Mammary Epithelial Cells Cancer Res., January 1, 2008; 68(1): 89 - 97. [Abstract] [Full Text] [PDF]
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 N. K. Kolas, J. R. Chapman, S. Nakada, J. Ylanko, R. Chahwan, F. D. Sweeney, S. Panier, M. Mendez, J. Wildenhain, T. M. Thomson, et al. Orchestration of the DNA-Damage Response by the RNF8 Ubiquitin Ligase Science, December 7, 2007; 318(5856): 1637 - 1640. [Abstract] [Full Text] [PDF]
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K. E. Gurley and C. J. Kemp Ataxia-Telangiectasia Mutated Is Not Required for p53 Induction and Apoptosis in Irradiated Epithelial Tissues Mol. Cancer Res., December 1, 2007; 5(12): 1312 - 1318. [Abstract] [Full Text] [PDF]
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 S. Hanasoge and M. Ljungman H2AX phosphorylation after UV irradiation is triggered by DNA repair intermediates and is mediated by the ATR kinase Carcinogenesis, November 1, 2007; 28(11): 2298 - 2304. [Abstract] [Full Text] [PDF]
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B. E. Lally, G. A. Geiger, S. Kridel, A. E. Arcury-Quandt, M. E. Robbins, N. D. Kock, K. Wheeler, P. Peddi, A. Georgakilas, G. D. Kao, et al. Identification and Biological Evaluation of a Novel and Potent Small Molecule Radiation Sensitizer via an Unbiased Screen of a Chemical Library Cancer Res., September 15, 2007; 67(18): 8791 - 8799. [Abstract] [Full Text] [PDF]
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I. M. Munoz, P. A. Jowsey, R. Toth, and J. Rouse Phospho-epitope binding by the BRCT domains of hPTIP controls multiple aspects of the cellular response to DNA damage Nucleic Acids Res., August 13, 2007; 35(16): 5312 - 5322. [Abstract] [Full Text] [PDF]
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G. D. Kao, Z. Jiang, A. M. Fernandes, A. K. Gupta, and A. Maity Inhibition of Phosphatidylinositol-3-OH Kinase/Akt Signaling Impairs DNA Repair in Glioblastoma Cells following Ionizing Radiation J. Biol. Chem., July 20, 2007; 282(29): 21206 - 21212. [Abstract] [Full Text] [PDF]
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S. Kuhfittig-Kulle, E. Feldmann, A. Odersky, A. Kuliczkowska, W. Goedecke, A. Eggert, and P. Pfeiffer The mutagenic potential of non-homologous end joining in the absence of the NHEJ core factors Ku70/80, DNA-PKcs and XRCC4-LigIV Mutagenesis, May 1, 2007; 22(3): 217 - 233. [Abstract] [Full Text] [PDF]
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X. Song, E. Gjoneska, Q. Ren, S. D. Taverna, C. D. Allis, and M. A. Gorovsky Phosphorylation of the SQ H2A.X Motif Is Required for Proper Meiosis and Mitosis in Tetrahymena thermophila Mol. Cell. Biol., April 1, 2007; 27(7): 2648 - 2660. [Abstract] [Full Text] [PDF]
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Y. Shi, G. E. Dodson, P. S. Mukhopadhyay, N. P. Shanware, A. T. Trinh, and R. S. Tibbetts Identification of Carboxyl-terminal MCM3 Phosphorylation Sites Using Polyreactive Phosphospecific Antibodies J. Biol. Chem., March 23, 2007; 282(12): 9236 - 9243. [Abstract] [Full Text] [PDF]
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 M. Matsumoto, K. Yaginuma, A. Igarashi, M. Imura, M. Hasegawa, K. Iwabuchi, T. Date, T. Mori, K. Ishizaki, K. Yamashita, et al. Perturbed gap-filling synthesis in nucleotide excision repair causes histone H2AX phosphorylation in human quiescent cells J. Cell Sci., March 15, 2007; 120(6): 1104 - 1112. [Abstract] [Full Text] [PDF]
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I. H. Ismail, T. I. Wadhra, and O. Hammarsten An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans Nucleic Acids Res., March 12, 2007; 35(5): e36 - e36. [Abstract] [Full Text] [PDF]
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S. E. Golding, E. Rosenberg, S. Neill, P. Dent, L. F. Povirk, and K. Valerie Extracellular Signal-Related Kinase Positively Regulates Ataxia Telangiectasia Mutated, Homologous Recombination Repair, and the DNA Damage Response Cancer Res., February 1, 2007; 67(3): 1046 - 1053. [Abstract] [Full Text] [PDF]
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 Q. Luo, J. Yang, Q.-L. Zeng, X.-M. Zhu, Y.-L. Qian, and H.-F. Huang 50-Hertz Electromagnetic Fields Induce gammaH2AX Foci Formation in Mouse Preimplantation Embryos In Vitro Biol Reprod, November 1, 2006; 75(5): 673 - 680. [Abstract] [Full Text] [PDF]
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 S. V. Diaz-Perez, D. O. Ferguson, C. Wang, G. Csankovszki, C. Wang, S.-C. Tsai, D. Dutta, V. Perez, S. Kim, C. D. Eller, et al. A Deletion at the Mouse Xist Gene Exposes Trans-effects That Alter the Heterochromatin of the Inactive X Chromosome and the Replication Time and DNA Stability of Both X Chromosomes Genetics, November 1, 2006; 174(3): 1115 - 1133. [Abstract] [Full Text] [PDF]
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M. S. Eller, X. Liao, S. Liu, K. Hanna, H. Backvall, P. L. Opresko, V. A. Bohr, and B. A. Gilchrest A role for WRN in telomere-based DNA damage responses PNAS, October 10, 2006; 103(41): 15073 - 15078. [Abstract] [Full Text] [PDF]
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Y. Joe, J.-H. Jeong, S. Yang, H. Kang, N. Motoyama, P. P. Pandolfi, J. H. Chung, and M. K. Kim ATR, PML, and CHK2 Play a Role in Arsenic Trioxide-induced Apoptosis J. Biol. Chem., September 29, 2006; 281(39): 28764 - 28771. [Abstract] [Full Text] [PDF]
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S. Liu, S. Bekker-Jensen, N. Mailand, C. Lukas, J. Bartek, and J. Lukas Claspin Operates Downstream of TopBP1 To Direct ATR Signaling towards Chk1 Activation. Mol. Cell. Biol., August 1, 2006; 26(16): 6056 - 6064. [Abstract] [Full Text] [PDF]
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M. Toulany, U. Kasten-Pisula, I. Brammer, S. Wang, J. Chen, K. Dittmann, M. Baumann, E. Dikomey, and H. P. Rodemann Blockage of Epidermal Growth Factor Receptor-Phosphatidylinositol 3-Kinase-AKT Signaling Increases Radiosensitivity of K-RAS Mutated Human Tumor Cells In vitro by Affecting DNA Repair. Clin. Cancer Res., July 1, 2006; 12(13): 4119 - 4126. [Abstract] [Full Text] [PDF]
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M. G. Rosenfeld, V. V. Lunyak, and C. K. Glass Sensors and signals: a coactivator/corepressor/epigenetic code for integrating signal-dependent programs of transcriptional response Genes & Dev., June 1, 2006; 20(11): 1405 - 1428. [Abstract] [Full Text] [PDF]
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Y. Zhao, H. D. Thomas, M. A. Batey, I. G. Cowell, C. J. Richardson, R. J. Griffin, A. H. Calvert, D. R. Newell, G. C.M. Smith, and N. J. Curtin Preclinical Evaluation of a Potent Novel DNA-Dependent Protein Kinase Inhibitor NU7441. Cancer Res., May 15, 2006; 66(10): 5354 - 5362. [Abstract] [Full Text] [PDF]
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C. M. Sturgeon, Z. A. Knight, K. M. Shokat, and M. Roberge Effect of combined DNA repair inhibition and G2 checkpoint inhibition on cell cycle progression after DNA damage. Mol. Cancer Ther., April 1, 2006; 5(4): 885 - 892. [Abstract] [Full Text] [PDF]
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 R. Sakasai, K. Shinohe, Y. Ichijima, N. Okita, A. Shibata, K. Asahina, and H. Teraoka Differential involvement of phosphatidylinositol 3-kinase-related protein kinases in hyperphosphorylation of replication protein A2 in response to replication-mediated DNA double-strand breaks Genes Cells, March 1, 2006; 11(3): 237 - 246. [Abstract] [Full Text] [PDF]
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T. Nubel, J. Damrot, W. P. Roos, B. Kaina, and G. Fritz Lovastatin Protects Human Endothelial Cells from Killing by Ionizing Radiation without Impairing Induction and Repair of DNA Double-Strand Breaks Clin. Cancer Res., February 1, 2006; 12(3): 933 - 939. [Abstract] [Full Text] [PDF]
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R. A. Greenberg, B. Sobhian, S. Pathania, S. B. Cantor, Y. Nakatani, and D. M. Livingston Multifactorial contributions to an acute DNA damage response by BRCA1/BARD1-containing complexes Genes & Dev., January 1, 2006; 20(1): 34 - 46. [Abstract] [Full Text] [PDF]
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S. T. Al Rashid, G. Dellaire, A. Cuddihy, F. Jalali, M. Vaid, C. Coackley, M. Folkard, Y. Xu, B. P.C. Chen, D. J. Chen, et al. Evidence for the Direct Binding of Phosphorylated p53 to Sites of DNA Breaks In vivo Cancer Res., December 1, 2005; 65(23): 10810 - 10821. [Abstract] [Full Text] [PDF]
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J. Li and D. F. Stern DNA Damage Regulates Chk2 Association with Chromatin J. Biol. Chem., November 11, 2005; 280(45): 37948 - 37956. [Abstract] [Full Text] [PDF]
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 K. J. McManus and M. J. Hendzel ATM-dependent DNA Damage-independent Mitotic Phosphorylation of H2AX in Normally Growing Mammalian Cells Mol. Biol. Cell, October 1, 2005; 16(10): 5013 - 5025. [Abstract] [Full Text] [PDF]
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Y. Sun, X. Jiang, S. Chen, N. Fernandes, and B. D. Price A role for the Tip60 histone acetyltransferase in the acetylation and activation of ATM PNAS, September 13, 2005; 102(37): 13182 - 13187. [Abstract] [Full Text] [PDF]
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M. Livingstone, H. Ruan, J. Weiner, K. R. Clauser, P. Strack, S. Jin, A. Williams, H. Greulich, J. Gardner, M. Venere, et al. Valosin-Containing Protein Phosphorylation at Ser784 in Response to DNA Damage Cancer Res., September 1, 2005; 65(17): 7533 - 7540. [Abstract] [Full Text] [PDF]
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X. Liu, Y. Guo, Y. Li, Y. Jiang, S. Chubb, A. Azuma, P. Huang, A. Matsuda, W. Hittelman, and W. Plunkett Molecular Basis for G2 Arrest Induced by 2'-C-Cyano-2'-Deoxy-1-{beta}-D-Arabino-Pentofuranosylcytosine and Consequences of Checkpoint Abrogation Cancer Res., August 1, 2005; 65(15): 6874 - 6881. [Abstract] [Full Text] [PDF]
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M. A. Bellani, P. J. Romanienko, D. A. Cairatti, and R. D. Camerini-Otero SPO11 is required for sex-body formation, and Spo11 heterozygosity rescues the prophase arrest of Atm-/- spermatocytes J. Cell Sci., August 1, 2005; 118(15): 3233 - 3245. [Abstract] [Full Text] [PDF]
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Y. Zhang, C. U.K. Lim, E. S. Williams, J. Zhou, Q. Zhang, M. H. Fox, S. M. Bailey, and H. L. Liber NBS1 Knockdown by Small Interfering RNA Increases Ionizing Radiation Mutagenesis and Telomere Association in Human Cells Cancer Res., July 1, 2005; 65(13): 5544 - 5553. [Abstract] [Full Text] [PDF]
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 L. Erker, R. Schubert, H. Yakushiji, C. Barlow, D. Larson, J. B. Mitchell, and A. Wynshaw-Boris Cancer chemoprevention by the antioxidant tempol acts partially via the p53 tumor suppressor Hum. Mol. Genet., June 15, 2005; 14(12): 1699 - 1708. [Abstract] [Full Text] [PDF]
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S. Dhanalakshmi, C. Agarwal, R. P. Singh, and R. Agarwal Silibinin Up-regulates DNA-Protein Kinase-dependent p53 Activation to Enhance UVB-induced Apoptosis in Mouse Epithelial JB6 Cells J. Biol. Chem., May 27, 2005; 280(21): 20375 - 20383. [Abstract] [Full Text] [PDF]
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 R. Dip and H. Naegeli More than just strand breaks: the recognition of structural DNA discontinuities by DNA-dependent protein kinase catalytic subunit FASEB J, May 1, 2005; 19(7): 704 - 715. [Abstract] [Full Text] [PDF]
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E. Bilsland and J. A. Downs Tails of histones in DNA double-strand break repair Mutagenesis, May 1, 2005; 20(3): 153 - 163. [Abstract] [Full Text] [PDF]
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J. D. Friesner, B. Liu, K. Culligan, and A. B. Britt Ionizing Radiation-dependent {gamma}-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related Mol. Biol. Cell, May 1, 2005; 16(5): 2566 - 2576. [Abstract] [Full Text] [PDF]
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D. G. Pankratz and S. L. Forsburg Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest Mol. Biol. Cell, April 1, 2005; 16(4): 1651 - 1660. [Abstract] [Full Text] [PDF]
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J. Li and D. F. Stern Regulation of CHK2 by DNA-dependent Protein Kinase J. Biol. Chem., March 25, 2005; 280(12): 12041 - 12050. [Abstract] [Full Text] [PDF]
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E. E. Bosco and E. S. Knudsen Differential role of RB in response to UV and IR damage Nucleic Acids Res., March 14, 2005; 33(5): 1581 - 1592. [Abstract] [Full Text] [PDF]
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J. Drouet, C. Delteil, J. Lefrancois, P. Concannon, B. Salles, and P. Calsou DNA-dependent Protein Kinase and XRCC4-DNA Ligase IV Mobilization in the Cell in Response to DNA Double Strand Breaks J. Biol. Chem., February 25, 2005; 280(8): 7060 - 7069. [Abstract] [Full Text] [PDF]
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S. Rooney, F. W. Alt, J. Sekiguchi, and J. P. Manis Artemis-independent functions of DNA-dependent protein kinase in Ig heavy chain class switch recombination and development PNAS, February 15, 2005; 102(7): 2471 - 2475. [Abstract] [Full Text] [PDF]
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J. G. Viniegra, N. Martinez, P. Modirassari, J. H. Losa, C. P. Cobo, V. J. S.-A. Lobo, C. I. A. Luquero, L. Alvarez-Vallina, S. Ramon y Cajal, J. M. Rojas, et al. Full Activation of PKB/Akt in Response to Insulin or Ionizing Radiation Is Mediated through ATM J. Biol. Chem., February 11, 2005; 280(6): 4029 - 4036. [Abstract] [Full Text] [PDF]
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I. H. Ismail, S. Nystrom, J. Nygren, and O. Hammarsten Activation of Ataxia Telangiectasia Mutated by DNA Strand Break-inducing Agents Correlates Closely with the Number of DNA Double Strand Breaks J. Biol. Chem., February 11, 2005; 280(6): 4649 - 4655. [Abstract] [Full Text] [PDF]
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B. Kysela, M. Chovanec, and P. A. Jeggo Phosphorylation of linker histones by DNA-dependent protein kinase is required for DNA ligase IV-dependent ligation in the presence of histone H1 PNAS, February 8, 2005; 102(6): 1877 - 1882. [Abstract] [Full Text] [PDF]
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M. Fernet, M. Gribaa, M. A.M. Salih, M. Z. Seidahmed, J. Hall, and M. Koenig Identification and functional consequences of a novel MRE11 mutation affecting 10 Saudi Arabian patients with the ataxia telangiectasia-like disorder Hum. Mol. Genet., January 15, 2005; 14(2): 307 - 318. [Abstract] [Full Text] [PDF]
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C. Bavoux, A. M. Leopoldino, V. Bergoglio, J. O-Wang, T. Ogi, A. Bieth, J.-G. Judde, S. D. J. Pena, M.-F. Poupon, T. Helleday, et al. Up-Regulation of the Error-Prone DNA Polymerase {kappa} Promotes Pleiotropic Genetic Alterations and Tumorigenesis Cancer Res., January 1, 2005; 65(1): 325 - 330. [Abstract] [Full Text] [PDF]
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E. U. Kurz, P. Douglas, and S. P. Lees-Miller Doxorubicin Activates ATM-dependent Phosphorylation of Multiple Downstream Targets in Part through the Generation of Reactive Oxygen Species J. Biol. Chem., December 17, 2004; 279(51): 53272 - 53281. [Abstract] [Full Text] [PDF]
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I. Hickson, Y. Zhao, C. J. Richardson, S. J. Green, N. M. B. Martin, A. I. Orr, P. M. Reaper, S. P. Jackson, N. J. Curtin, and G. C. M. Smith Identification and Characterization of a Novel and Specific Inhibitor of the Ataxia-Telangiectasia Mutated Kinase ATM Cancer Res., December 15, 2004; 64(24): 9152 - 9159. [Abstract] [Full Text] [PDF]
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S. J. Collis, J. M. Schwaninger, A. J. Ntambi, T. W. Keller, W. G. Nelson, L. E. Dillehay, and T. L. DeWeese Evasion of Early Cellular Response Mechanisms following Low Level Radiation-induced DNA Damage J. Biol. Chem., November 26, 2004; 279(48): 49624 - 49632. [Abstract] [Full Text] [PDF]
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B. Reina-San-Martin, H. T. Chen, A. Nussenzweig, and M. C. Nussenzweig ATM Is Required for Efficient Recombination between Immunoglobulin Switch Regions J. Exp. Med., November 1, 2004; 200(9): 1103 - 1110. [Abstract] [Full Text] [PDF]
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R. Daniel, J. Ramcharan, E. Rogakou, K. D. Taganov, J. G. Greger, W. Bonner, A. Nussenzweig, R. A. Katz, and A. M. Skalka Histone H2AX Is Phosphorylated at Sites of Retroviral DNA Integration but Is Dispensable for Postintegration Repair J. Biol. Chem., October 29, 2004; 279(44): 45810 - 45814. [Abstract] [Full Text] [PDF]
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