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Apoptosis of Prostate Cancer Cells by CKIs

View larger version (19K): [in this window] [in a new window] [Download PPT slide]   Figure 5. Requirement for p53 for efficient apoptosis of CKI-treated prostate cancer cells. A, LNCaP cells received no addition (NA) or 30 µmol/L pifithrin- (PTF) for 24 hours. Cells subsequently received DMSO (control, C) or 25 µmol/L roscovitine (ROS) for 16 hours. Amounts of p53 and cleaved PARP were determined by Western blotting of cell extracts. B, LNCaP and PC3 cells received the indicated concentrations of roscovitine for 20 hours. Amounts of cytosolic, histone-associated DNA fragments were determined by a cell death detection ELISA. Bars, SD. bgd, background (buffer only). C, LNCaP and PC3 cells received the indicated concentrations of CGP74514A for 20 hours. Amounts of cleaved PARP and XIAP were determined by Western blotting of cell extracts. D, LNCaP and DU145 cells received the indicated concentrations of roscovitine for 20 hours. Amounts of cleaved PARP and XIAP were determined by Western blotting of cell extracts.

1. Mohapatra S, Chu B, Zhao X, Pledger WJ. Accumulation of p53 and reductions in XIAP abundance promote the apoptosis of prostate cancer cells. Cancer Res2005;65:7717–23.[Abstract/Free Full Text]

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