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Cell culture. Two normal human mammary epithelial cells (N1330 HMEC and HMEpC) were from BioWittaker, Inc. (Walkersfield, MD) and Cell Applications, Inc. (San Diego, CA) and were maintained in defined mammary epithelial cell medium provided by the respective companies. All other cell lines used were maintained in RPMI containing 10% FCS. Cultures were determined to be Mycoplasma-free using the GeneProbe rapid detection system.
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