Cell Cycle Regulation by MicroRNAs in Embryonic Stem Cells

¹The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research; ²Center for Reproductive Sciences; and ³Department of Urology, University of California, San Francisco, San Francisco, California

^* To whom correspondence should be addressed. E-mail: BlellochR{at}stemcell.ucsf.edu.

	Abstract

The cell cycle is tightly orchestrated during normal development. Embryonic stem (ES) cells have a unique cell cycle structure, in which the G1/S restriction is largely absent, enabling cells to rapidly move through the G1 phase and enter the S phase. This hastened cell cycle allows the early embryo to rapidly grow. Recent experiments suggest that small noncoding RNAs, the microRNAs (miRNAs), play a central role in achieving this unique cell cycle structure. The responsible miRNAs function by suppressing multiple inhibitors of the G1/S transition. Expression of these miRNAs drops dramatically as the ES cells differentiate and as the G1 phase extends. Some of the same miRNAs are overexpressed in cancers, in which they can promote tumor growth, suggesting common mechanisms of miRNA-regulated cell cycle control in ES cells and cancers. This review discusses these recent findings in the context of broader knowledge of cell cycle control in normal and abnormal development. [Cancer Res 2009;69(10):4093–6]