Summary
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The oxidation of formate in dialyzed mouse liver homogenates has been found to be accelerated by additions of acetaldehyde, as well as hypoxanthine and inosine. The greatest stimulation was obtained with inosine.
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The effect of formate concentration on the rate of oxidation has been studied in homogenates optimally supplemented with inosine. Although most of the concentration range can be described by the Michaelis-Menten equation, the data also indicate that generation of hydrogen peroxide becomes limiting at formate concentrations of around 0.1 M.
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The oxidation of formate is decreased by 40–50 per cent in fortified homogenates from mice carrying the Ehrlich ascites cell carcinoma.
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A decreased rate of oxidation of methionine to formate has been found in liver prisms from tumor-bearing mice as compared with those from normal mice.
Footnotes
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↵* Supported by an Institutional Grant from the American Cancer Society and by a research grant from the National Cancer Institute (C-2340), Dept. of Health, Education, and Welfare.
A preliminary report of part of this work has been published (20).
Taken from a dissertation by A. M. S. presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy to the Graduate School of the University of Southern California.
- Received June 2, 1958.
- ©1958 American Association for Cancer Research.
Volume 18, Issue 10
