Microdiver Studies on the Respiration of Burkitt Lymphoma Cells (EB-3)

Summary

The effects of several sugars on the respiration of Burkitt lymphoma cells (EB-3) were studied by the micro-Cartesian diver technique. Results demonstrated that respiration was inhibited by glucose, fructose, and mannose, whereas galactose and sucrose produced no effects. Inhibition was more pronounced at higher concentrations of glucose and fructose. Respiratory inhibition was maintained after 4 hr of determination for the glucose- and fructose-exposed cells, and respiration declined for cells in the absence of substrate. The effects obtained with the sugars are considered to be due to the hexokinase-type substrates and the general metabolic characteristics of the cells.