Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Articles

Tumor-blocking and -inhibitory Serum Factors in the Clinical Course of Canine Venereal Tumor

B. Taylor Bennett, Kim M. Debelak-Fehir and Robert B. Epstein
B. Taylor Bennett
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kim M. Debelak-Fehir
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Robert B. Epstein
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published November 1975
  • Article
  • Info & Metrics
  • PDF
Loading

Summary

An in vitro method for growing colonies of canine transplantable venereal tumor cells in a semisolid agar medium is described. Using autologous or pooled homologous normal dog sera as a feeder layer, 49.3 ± 3.5 and 47.5 ± 4.5 tumor colonies were obtained, respectively, when 2 × 104 tumor cells were plated. With this assay system, assessment of colony counts provided an accurate and rapid technique for monitoring serum factors in tumor-bearing dogs that inhibited colony formation, or blocked the inhibition of colony formation. In 52 dogs given tumor transplants, a direct correlation was demonstrated between serum activity tested in vitro and the in vivo growth characteristics of the tumor. Tumor cells preincubated with serum from dogs with active tumor growth consistently showed normal colony growth when cultured in agar containing colony-inhibitory sera (blocking effect). In vivo regression was characterized by loss of serum blocking and the development of serum colony-inhibitory activity in culture. In metastatic disease, only blocking activity could be identified, while persistent local invasive disease was characterized by low levels of both blocking and inhibitory serum activity. The sensitivity of this technique coupled with its in vivo predictive capabilities provides a model for monitoring serological responses to a naturally occurring neoplasm in a large, randomly bred animal.

Footnotes

  • ↵1 This work was supported by Basic Institutional Support Funds, Veterans Administration West Side Hospital FCP 632/103 and the Illinois Division of The American Cancer Society GPF 15. Presented in part at the American Association for Cancer Research, Houston, Texas, March 1974.

  • Received March 20, 1975.
  • Accepted July 18, 1975.
  • ©1975 American Association for Cancer Research.
PreviousNext
Back to top
November 1975
Volume 35, Issue 11
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author

Sign up for alerts

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Tumor-blocking and -inhibitory Serum Factors in the Clinical Course of Canine Venereal Tumor
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Tumor-blocking and -inhibitory Serum Factors in the Clinical Course of Canine Venereal Tumor
B. Taylor Bennett, Kim M. Debelak-Fehir and Robert B. Epstein
Cancer Res November 1 1975 (35) (11) 2942-2947;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Tumor-blocking and -inhibitory Serum Factors in the Clinical Course of Canine Venereal Tumor
B. Taylor Bennett, Kim M. Debelak-Fehir and Robert B. Epstein
Cancer Res November 1 1975 (35) (11) 2942-2947;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Intersections between Blood Cell Development and Leukemia Genes
  • Genetic Control of Programmed Cell Death in the Nematode Caenorhabditis elegans
  • Core-Binding Factor: A Central Player in Hematopoiesis and Leukemia
Show more Articles
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement