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A Transient Thermotolerant Survival Response Produced by Single Thermal Doses in HeLa Cells

Eugene W. Gerner, Robert Boone, William G. Connor, John A. Hicks and Max L. M. Boone
Eugene W. Gerner
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Robert Boone
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William G. Connor
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John A. Hicks
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Max L. M. Boone
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DOI:  Published March 1976
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Summary

Continuous exposure of HeLa cells in culture to elevated temperatures (41–45°) results in cell killing which increases exponentially as the time at the elevated temperature increases linearly. When cells are returned to 37° after an initial thermal dose, cellular sensitivity to subsequent hyperthermic doses is reduced. Cell inactivation rates for cultures previously treated with 44° for either 0.5 or 1 hr followed by incubation at 37° for 2 hr, showed D0's of 1.1 and 1.5 hr, respectively, for subsequent thermal treatments at 44°. Cultures receiving no prior hyperthermic dose had a D0 of 0.5 hr for treatments at 44° for up to 3.5 hr. The viable progeny of cells treated with 44° for 1 hr, however, had the same sensitivity to thermal doses at 44° as did previously unheated cells. These results and others demonstrate that (a) single thermal doses produce a state of thermotolerance in HeLa cells to subsequent hyperthermic doses; (b) the degree of thermotolerance produced is dependent on the magnitude (i.e., temperature and time at the elevated temperature) of the first thermal dose; (c) thermotolerance does not develop at the elevated temperature but requires a return of culture temperatures to 37°; (d) cellular acquisition of thermal tolerance is dependent on cell metabolism, as demonstrated by an inhibition of the effect at 0°; and (e) this effect is a transient phenomenon which is lost as cells divide following the first thermal dose.

Footnotes

  • ↵1 This work was supported by Grants from the American Cancer Society (In-110), the USPHS (IN-3150-80), and the National Cancer Institute (CA 17343, CA 18273).

  • ↵2 To whom requests for reprints should be addressed.

  • Received July 10, 1975.
  • Accepted December 5, 1975.
  • ©1976 American Association for Cancer Research.
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March 1976
Volume 36, Issue 3
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A Transient Thermotolerant Survival Response Produced by Single Thermal Doses in HeLa Cells
Eugene W. Gerner, Robert Boone, William G. Connor, John A. Hicks and Max L. M. Boone
Cancer Res March 1 1976 (36) (3) 1035-1040;

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A Transient Thermotolerant Survival Response Produced by Single Thermal Doses in HeLa Cells
Eugene W. Gerner, Robert Boone, William G. Connor, John A. Hicks and Max L. M. Boone
Cancer Res March 1 1976 (36) (3) 1035-1040;
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Cancer Research Online ISSN: 1538-7445
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