Abstract
Carcinogenicity, toxicity, and mutagenicity of the milk of bracken fern (BF)-fed cows were determined. Four Brown Swiss cows were fed a BF-containing diet, and two cows were fed a normal hay-grain diet. The milk obtained was fed to rats as either fresh or freeze-dried powdered milk mixed with grain diet: 9 of 34 fed whole milk and 11 of 56 fed powdered milk diet developed small Intestine, kidney, or urinary bladder carcinomas within 117 weeks, while 0 of 70 rats fed either whole or powdered milk from cows receiving a normal diet and 0 of 20 rats fed a basic grain diet displayed neoplasia of these organs.
Six organic solvent extract fractions of milk were prepared from BF- or normal diet-fed cows. Diethyl ehter and ehtyl acetate fractions displayed the highest murine acute toxicity. The murine bladder carcinogenicity of prepared fractions was assessed by the pellet implantation technqiue. Diethyl ether fractions from milk of BF-fed cows induced bladder carcinoma in 10 of 24 mice compared witht he corresponding fraction of normal diet-fed cows (3 of 19) or mice exposed to only the cholesterol vehicle (5 of 30, 6 of 40) (p < 0.01).
Four organic solvent extract fractions of milk obtained from BF- or normal diet-fed cows were prepared and tested for mutagenic activity in Salmonella typhimurium TA 98 and TA 100. The chloroform:methanol fraction from BF-fed cows demonstrated mutagenic activity for TA 100 but not for TA 98.
Footnotes
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↵1 Supported in part by USPHS Research Grants CA 10017, CA 14520, and CA 14523. A preliminary report of this work was presented (8).
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↵2 To whom requests for repritns should be addressed, at Division of Clinical Oncology, Department of Human Oncology, University of Wisconsin Center for Health Sciences, 1300 University Avenue, Madison, Wis. 53706.
- Received October 11, 1977.
- Accepted March 7, 1978.
- ©1978 American Association for Cancer Research.