Abstract
This study was undertaken to define the incidence and concentration of uncharged nuclear estrogen receptors (RN) in human breast cancer. The concentrations of RN and cytoplasmic uncharged receptor were determined on sucrose gradients following a 4-hr incubation at 4° with 1.6 nm 17β-[3H]estradiol in 139 tumor specimens from 137 patients. RN was extracted from washed nuclear pellets in buffer containing 0.4 m KCl. The receptor molecule extracted had a high affinity for 17β-[3H]estradiol (Kd = 0.9 to 7.6 nm) and was specific for estrogen. The possibility of artifact due to cytoplasmic contamination of the nuclear fraction or high-ionic-strength-induced exchange of charged nuclear receptors was rendered unlikely by validation experiments performed with pooled tumor tissue. Significant amounts of cytoplasmic uncharged receptor (greater than 7 fmol/mg protein) were found in 63.3% of the tumors. Similar significant amounts of RN were found in 29.5% of the tumors. Significant amounts of RN in the presence of undetectable cytoplasmic uncharged receptor were found in 2.9% of the tumors. The percentage of tumors that contain significant amounts of RN is approximately the same percentage of estrogen receptor-positive tumors that do not respond to ablative therapy.
Footnotes
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↵2 To whom requests for reprints should be addressed.
- Received December 2, 1977.
- Accepted March 27, 1978.
- ©1978 American Association for Cancer Research.
Volume 38, Issue 7
