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Basic Sciences

Characterization of Interleukin 2-dependent Cytotoxic T-Cell Clones: Specificity, Cell Surface Phenotype, and Susceptibility to Blocking by Lyt Antisera

Michael A. Palladino, Yuichi Obata, Elisabeth Stockert and Herbert F. Oettgen
Michael A. Palladino
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Yuichi Obata
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Elisabeth Stockert
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Herbert F. Oettgen
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DOI:  Published February 1983
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Abstract

Cytotoxic T-cells were derived from the peritoneal cavity of a C57BL/6 mouse immunized with BALB/c sarcoma Meth A and from the spleens of BALB/c × C57BL/6 F1 (hereafter called CB6F1) mice immunized with BALB/c leukemia RL♂1. The cells were cultured in interleukin 2 and cloned by limiting dilution, and their specificity was determined by direct tests and competitive inhibition assays. C57BL/6 anti-Meth A effector cells recognized H-2Dd determinants. CB6F1 anti-RL♂1 effector cells recognized a unique cell surface antigen of leukemia RL♂1. The specificity was maintained in long-term culture. The cell surface phenotype of the cloned effector cell lines as determined by absorption analysis was Thy-1.2+, Lyt-1.2+, 2.2+, and 3.2+. Cytotoxicity was blocked at the target cell level by antisera against H-2Dd, but not H-2Dk or H-2b, and at the effector cell level by antisera against Lyt-2.2 and 3.2, but not Lyt-1.2, Ly-5.1 or Thy-1.2.

Footnotes

  • ↵1 Research supported by American Cancer Society Grants IM-162C, IM-310, and JFRA-39 and by USPHS Grants CA-08748 and CA-16599 awarded by the National Cancer Institute, Department of Health and Human Services.

  • Received February 8, 1982.
  • Accepted October 6, 1982.
  • ©1983 American Association for Cancer Research.
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February 1983
Volume 43, Issue 2
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Characterization of Interleukin 2-dependent Cytotoxic T-Cell Clones: Specificity, Cell Surface Phenotype, and Susceptibility to Blocking by Lyt Antisera
Michael A. Palladino, Yuichi Obata, Elisabeth Stockert and Herbert F. Oettgen
Cancer Res February 1 1983 (43) (2) 572-576;

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Characterization of Interleukin 2-dependent Cytotoxic T-Cell Clones: Specificity, Cell Surface Phenotype, and Susceptibility to Blocking by Lyt Antisera
Michael A. Palladino, Yuichi Obata, Elisabeth Stockert and Herbert F. Oettgen
Cancer Res February 1 1983 (43) (2) 572-576;
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