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Basic Sciences

Establishment and Characterization of Four Human Bladder Tumor Cell Lines and Sublines with Different Degrees of Malignancy

Chi-Wei Lin, Julia C. Lin and George R. Prout Jr.
Chi-Wei Lin
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Julia C. Lin
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George R. Prout
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DOI:  Published October 1985
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Abstract

We have established four human bladder tumor cultures, designated MGH-U1 to -U4 (also known as EJ, HM, RN, and RB in some previous reports). All have been grown in culture for over 30 passages and were free of Mycoplasma contamination.

Characterizations of these cell lines were performed. These include isozyme profile, morphology with light and scanning electron microscopes, karyotype, growth rate, DNA content by flow cytometry, presence of cell surface ABH isoantigens, tumorigenicity in nude mice, lactic acid dehydrogenase isozymes, and colony formation in soft agar.

Results obtained from these characterizations confirm that MGH-U1 and -U2 are sublines of a previously established bladder tumor cell line, T-24. These results also show that MGH-U3 and -U4, derived respectively from a grade 1 tumor and an urothelium biopsy with severe atypia, are likely to be independent human bladder cell lines and different from other transitional cell bladder carcinoma cell lines reported. The study further demonstrates that these four cell lines/sublines have different degrees of malignancy and a close correlation, in biological and malignant characteristics, between the cells in culture and those in the original tumors. Therefore these cultures may represent cells at different stages of malignant progression. These can be useful models for studies of the development and progression of bladder tumors and detection and treatment of bladder tumors of different grades and stages.

Footnotes

  • ↵1 A portion of this study was reported at the 75th Annual Meeting of the American Association for Cancer Research (1).

  • ↵2 To whom requests for reprints should be addressed.

  • Received April 9, 1985.
  • Revision received June 24, 1985.
  • Accepted June 24, 1985.
  • ©1985 American Association for Cancer Research.
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October 1985
Volume 45, Issue 10
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Establishment and Characterization of Four Human Bladder Tumor Cell Lines and Sublines with Different Degrees of Malignancy
Chi-Wei Lin, Julia C. Lin and George R. Prout Jr.
Cancer Res October 1 1985 (45) (10) 5070-5079;

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Establishment and Characterization of Four Human Bladder Tumor Cell Lines and Sublines with Different Degrees of Malignancy
Chi-Wei Lin, Julia C. Lin and George R. Prout Jr.
Cancer Res October 1 1985 (45) (10) 5070-5079;
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