Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Basic Sciences

Catalysis of the Covalent Binding of 3-Hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole to DNA by a l-Proline- and Adenosine Triphosphate-dependent Enzyme in Rat Hepatic Cytosol

Yasushi Yamazoe, Miki Shimada, Atsuko Shinohara, Kazuki Saito, Tetsuya Kamataki and Ryuichi Kato
Yasushi Yamazoe
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miki Shimada
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Atsuko Shinohara
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuki Saito
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsuya Kamataki
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ryuichi Kato
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published June 1985
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

An enzymatic mechanism involved in the activation of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (N-hydroxy-Trp-P-2), a mutagenic intermediate of a tryptophan pyrolysate, was studied in vitro. In hepatic cytosol supplemented with adenosine triphosphate and l-proline, N-hydroxy-Trp-P-2 was converted to a form which reacts readily with DNA. The enzyme responsible for the activation was partially purified and identified as prolyl transfer RNA synthetase as judged by their cofactor requirements, inhibition by pyrophosphate or adenosine monophosphate, and copurification of their activities.

The prolyl transfer RNA-dependent covalent binding of N-hydroxy-Trp-P-2 to DNA of hepatic cytosol was highest in rats, followed by mice, hamsters, rabbits, and guinea pigs in that order. The capacity for the binding of N-hydroxy-Trp-P-2 was largely consistent with their prolyl transfer RNA synthetase activity.

With regard to the ultimate form of N-hydroxy-Trp-P-2 for the covalent binding, a possible formation of N,O-prolyl-3-amino-1-methyl-5H-pyrido[4,3-b]indole was proposed.

Footnotes

  • ↵1 This work was supported in part by Grants-in-Aid for Cancer Research from the Ministry of Health and Welfare of Japan, the Ministry of Education, Science and Culture of Japan, and an additional grant from the Princess Takamatsu Cancer Research Fund.

  • ↵2 To whom requests for reprints should be addressed.

  • Received August 6, 1984.
  • Revision received December 26, 1984.
  • Accepted February 28, 1985.
  • ©1985 American Association for Cancer Research.
PreviousNext
Back to top
June 1985
Volume 45, Issue 6
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)

Sign up for alerts

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Catalysis of the Covalent Binding of 3-Hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole to DNA by a l-Proline- and Adenosine Triphosphate-dependent Enzyme in Rat Hepatic Cytosol
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Catalysis of the Covalent Binding of 3-Hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole to DNA by a l-Proline- and Adenosine Triphosphate-dependent Enzyme in Rat Hepatic Cytosol
Yasushi Yamazoe, Miki Shimada, Atsuko Shinohara, Kazuki Saito, Tetsuya Kamataki and Ryuichi Kato
Cancer Res June 1 1985 (45) (6) 2495-2500;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Catalysis of the Covalent Binding of 3-Hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole to DNA by a l-Proline- and Adenosine Triphosphate-dependent Enzyme in Rat Hepatic Cytosol
Yasushi Yamazoe, Miki Shimada, Atsuko Shinohara, Kazuki Saito, Tetsuya Kamataki and Ryuichi Kato
Cancer Res June 1 1985 (45) (6) 2495-2500;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

Basic Sciences

  • Differential Expression of Transforming Growth Factor-β1 Gene in 3LL Metastatic Variants
  • Extracellular Matrix and the Patterns of Differentiation of Human Endometrial Carcinomas in Vitro and in Vivo
  • O6-Methylguanine Is a Critical Determinant of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone Tumorigenesis in A/J Mouse Lung
Show more 3

Articles

  • Differential Expression of Transforming Growth Factor-β1 Gene in 3LL Metastatic Variants
  • Extracellular Matrix and the Patterns of Differentiation of Human Endometrial Carcinomas in Vitro and in Vivo
  • O6-Methylguanine Is a Critical Determinant of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone Tumorigenesis in A/J Mouse Lung
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement