Abstract
The influence of pretreatment with piperonylbutoxide (PIP) on the biological effects of N-nitrosodiethylamine (DEN) in vivo in Syrian golden hamsters was investigated. PIP pretreatment significantly reduced covalent binding of N-[ethyl-1-14C]DEN to tissue macromolecules in trachea, lung, and liver, while it did not change the tissue distribution of the parent compound. In a chronic experiment, hamsters treated with PIP before each DEN injection did not develop any tumors or precancerous changes in the lungs, while 60% of the animals given DEN alone developed lung tumors with the morphology of Clara cells and endocrine cells. Tumor incidence in the trachea was also significantly reduced by PIP, but to a lesser extent than in the lungs.
Footnotes
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↵1 To whom requests for reprints should be addressed, at Laboratory of Experimental Therapeutics and Metabolism, Division of Cancer Treatment, National Cancer Institute, Bldg. 37, Room 5C11, Bethesda, MD 20205.
- Received July 24, 1984.
- Revision received October 31, 1984.
- Revision received January 25, 1985.
- Accepted March 8, 1985.
- ©1985 American Association for Cancer Research.
Volume 45, Issue 6
