Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Basic Sciences

Synergism and Antagonism in the Effects of 1α,25-Dihydroxyvitamin D3, Retinoic Acid, Dexamethasone, and a Tumor-promoting Phorbol Ester on the Functional Capability of P388D1 Cells: Phagocytosis and Transglutaminase Activity

Rachel Goldman
Rachel Goldman
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published July 1985
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and retinoic acid (RA) induce a high-phagocytic phenotype in the macrophage-like tumor cell line P388D1. A concurrent cultivation of P388D1 cells in the presence of suboptimal concentrations of both agents led to an extent of induction of phagocytic activity that surpassed the additive effect of either of the agents alone; i.e., 1,25(OH)2D3 and RA synergistically induce the phagocytic capability of P388D1 cells. Dexamethasone and 4β-phorbol-12β-myristate-13α-acetate (TPA) did not induce a high-phagocytic phenotype in P388D1 cells and affected differentially the high-phagocytic phenotype induced by RA and 1,25(OH)2D3. Dexamethasone inhibited the phagocytic activity induced by RA (80% at 24 h), while it had small suppressive effects on that induced by 1,25(OH)2D3. TPA suppressed the phagocytic activity induced by RA (60% within 96 h) while potentiating the expression of the high-phagocytic phenotype induced by 1,25(OH)2D3 (50% increase with 96 h). The observed effects did not involve modulation of prostaglandin synthesis or intracellular cyclic adenosine 3′:5′-monophosphate. Expression of transglutaminase activity in P388D1 cells was also modulated differentially by the four agents; 1,25(OH)2D3 treatment had no effect on enzyme level, RA and TPA suppressed it, and dexamethasone increased it. The data suggest that: (a) 1,25(OH)2D3 and RA act via disparate mechanisms that can operate simultaneously; (b) the elements induced in P388D1 cells by 1,25(OH)2D3 and RA, and which are responsible for the phagocytic activity, differ in their sensitivity to dexamethasone and TPA; and (c) transglutaminase activity in P388D1 cells is readily manipulable, but there seems to be no straightforward correlation between the level of its activity and the phagocytic capability of the cells or their rate of proliferation.

Footnotes

  • ↵1 Partially supported by the Edward D. and Anna Mitchell Research Fund.

  • ↵2 To whom requests for reprints should be addressed, at the Department of Membrane Research, The Weizmann Institute of Science, Rehovot 76100, Israel.

  • Received November 2, 1984.
  • Revision received March 4, 1985.
  • Accepted March 7, 1985.
  • ©1985 American Association for Cancer Research.
PreviousNext
Back to top
July 1985
Volume 45, Issue 7
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)

Sign up for alerts

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Synergism and Antagonism in the Effects of 1α,25-Dihydroxyvitamin D3, Retinoic Acid, Dexamethasone, and a Tumor-promoting Phorbol Ester on the Functional Capability of P388D1 Cells: Phagocytosis and Transglutaminase Activity
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Synergism and Antagonism in the Effects of 1α,25-Dihydroxyvitamin D3, Retinoic Acid, Dexamethasone, and a Tumor-promoting Phorbol Ester on the Functional Capability of P388D1 Cells: Phagocytosis and Transglutaminase Activity
Rachel Goldman
Cancer Res July 1 1985 (45) (7) 3118-3124;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Synergism and Antagonism in the Effects of 1α,25-Dihydroxyvitamin D3, Retinoic Acid, Dexamethasone, and a Tumor-promoting Phorbol Ester on the Functional Capability of P388D1 Cells: Phagocytosis and Transglutaminase Activity
Rachel Goldman
Cancer Res July 1 1985 (45) (7) 3118-3124;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

Basic Sciences

  • Dietary Calcium and Vitamin D Modulate 1,2-Dimethylhydrazine-induced Colonic Carcinogenesis in the Rat
  • Androgen Sensitivity of the New Human Breast Cancer Cell Line MFM-223
  • Transforming Growth Factor Gene Expression in Human Endometrial Adenocarcinoma Cells: Regulation by Progestins
Show more 3

Articles

  • Dietary Calcium and Vitamin D Modulate 1,2-Dimethylhydrazine-induced Colonic Carcinogenesis in the Rat
  • Androgen Sensitivity of the New Human Breast Cancer Cell Line MFM-223
  • Transforming Growth Factor Gene Expression in Human Endometrial Adenocarcinoma Cells: Regulation by Progestins
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement