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Clinical and Epidemiological Investigations

Histochemical Studies with an Estrogen Receptor-related Protein in Human Breast Tumors

Antonio Cano, Arnold I. Coffer, Remy Adatia, Rosemary R. Millis, Robert D. Rubens and Roger J. B. King
Antonio Cano
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Arnold I. Coffer
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Remy Adatia
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Rosemary R. Millis
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Robert D. Rubens
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Roger J. B. King
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DOI:  Published December 1986
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Abstract

The histochemical characteristics of a Mr 29,000 phosphoprotein related to estradiol receptor are described in a large series of human breast tumors. The antigen was detected with a monoclonal antibody (D5) raised against partially purified human myometrial estradiol receptor. An indirect immunoperoxidase method was used with methacarnfixed, wax-embedded sections. Quantitation of staining and its reproducibility are described. Results with trucut biopsies agree with those obtained with larger tumor sections. Normal breast is infrequently positive. Histochemical staining is higher in invasive carcinoma than in normal breast with ductal carcinoma in situ adjacent to infiltrating tumors exhibiting intermediate values. Furthermore, most in situ carcinomas have a heterogeneous staining pattern. About 20% of invasive tumors also exhibit heterogeneity. No simple correlation is seen between staining and histological grade. There are more low-staining tumors in young (<50 yr old) patients than in older women.

Staining correlates with levels of cytosol estradiol receptor but not cytosol progesterone receptor. However, cytosol estradiol receptor-negative, cytosol progesterone receptor-positive tumors tend to have positive Mr 29,000 phosphoprotein levels. Positive staining is associated with a higher response rate to hormone therapy (50%). None of the negative tumors responded to hormone treatment. With these patients, comparison of histochemical assay for Mr 29,000 phosphoprotein and [3H]estradiol binding assays indicated that the former was at least as good as the latter assay in predicting hormone response. About 20% of cytosol estradiol receptor-positive tumors have low Mr 29,000 phosphoprotein, and such tumors have poor response to hormone treatment.

Footnotes

  • ↵2 To whom requests for reprints should be addressed.

  • Received March 31, 1986.
  • Revision received June 24, 1986.
  • Revision received August 15, 1986.
  • Accepted August 22, 1986.
  • ©1986 American Association for Cancer Research.
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December 1986
Volume 46, Issue 12 Part 1
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Histochemical Studies with an Estrogen Receptor-related Protein in Human Breast Tumors
Antonio Cano, Arnold I. Coffer, Remy Adatia, Rosemary R. Millis, Robert D. Rubens and Roger J. B. King
Cancer Res December 1 1986 (46) (12 Part 1) 6475-6480;

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Histochemical Studies with an Estrogen Receptor-related Protein in Human Breast Tumors
Antonio Cano, Arnold I. Coffer, Remy Adatia, Rosemary R. Millis, Robert D. Rubens and Roger J. B. King
Cancer Res December 1 1986 (46) (12 Part 1) 6475-6480;
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