Sensitive and specific methods are needed for measuring human exposure to carcinogens. Synchronous fluorescence spectrophotometry can be used to measure fmol of aflatoxins, their metabolites, and DNA adducts. Computer-assisted analysis of spectra of these agents obtained by synchronous fluorescence spectrophotometry can be displayed as contour maps which are highly specific for each agent. Individual agents in mixtures, e.g. aflatoxins B1 and M1, can be identified by fourth derivative spectral analysis. This physical method should complement immunological and other methods to measure aflatoxin B1, its metabolites, and nucleic acid adducts.
↵1 To whom requests for reprints should be addressed, at National Cancer Institute, Building 37, Room 2C07, Bethesda, MD 20892.
- Received July 19, 1985.
- Revision received March 12, 1986.
- Accepted April 4, 1986.
- ©1986 American Association for Cancer Research.