Participation of Common Surface Receptor(s) in the Activation of Murine Macrophages by Sarcophaga Lectin and Wheat Germ Agglutinin

Abstract

Mouse peritoneal macrophage surface proteins which bind Sarcophaga lectin were studied. Two major binding proteins with molecular masses of 170 and 110 kDa were identified. Sarcophaga lectin and wheat germ agglutinin were found to share common binding proteins for activating macrophages, although their hapten sugars are different. Antibody raised against the Sarcophaga lectin-binding proteins inhibited both the production of tumor-specific cytotoxic protein by the macrophage-like cell line J774.1 cells and the lectin-dependent macrophage-mediated cytotoxic reaction induced by Sarcophaga lectin or wheat germ agglutinin. Thus the 170-kDa and/or 110-kDa protein is important in activation of macrophages.