Abstract
In the course of development of a “library” of monoclonal antibodies to nucleolar proteins, a monoclonal antibody to a nuclear antigen with a molecular weight of 150,000 was obtained. Using this monoclonal antibody as an immunocytochemical probe, low immunofluorescence was demonstrated in human peripheral blood lymphocytes or HL-60 cells treated with retinoic acid. In contrast, a high degree of immunofluorescence was detected in rapidly proliferating human cells (HeLa, Hep-2, HL-60), in Novikoff hepatoma cells, and in phytohemagglutinin-activated human blood lymphocytes. Immunodot blot analysis indicated there were low levels of Mr 150,000 protein in quiescent lymphocytes and increasing amounts after stimulation with phytohemagglutinin. The increased level of Mr 150,000 protein thus is associated with cell proliferation.
Footnotes
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↵1 These studies were supported by Cancer Research Center Grant CA-10893, P1, awarded by the National Cancer Institute, Department of Health and Human Services, USPHS; the DeBakey Medical Foundation; the H. Leland Kaplan Cancer Research Endowment; the Linda and Ronnie Finger Cancer Research Endowment Fund; and the William S. Farish Fund.
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↵2 To whom requests for reprints should be addressed.
- Received May 4, 1988.
- Revision received August 5, 1988.
- Accepted September 14, 1988.
- ©1988 American Association for Cancer Research.
Volume 48, Issue 24 Part 1
