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Basic Sciences

Effects of Serum, Transforming Growth Factor Type β, or 12-O-Tetradecanoylphorbol-13-acetate on Ionized Cytosolic Calcium Concentration in Normal and Transformed Human Bronchial Epithelial Cells

Masao Miyashita, Mary W. Smith, James C. Willey, John F. Lechner, Benjamin F. Trump and Curtis C. Harris
Masao Miyashita
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Mary W. Smith
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James C. Willey
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John F. Lechner
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Benjamin F. Trump
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Curtis C. Harris
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DOI:  Published January 1989
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Abstract

Fluctuations in ionized cytosolic calcium ([Ca2+]i) are considered important signals for induction of growth or differentiation in mammalian cells. The resting concentrations of [Ca2+]i in normal and adenovirus 12-SV40 hybrid virus-transformed (BEAS-2B) human bronchial epithelial cells were 63 ± 15 nM (SD) and 44 ± 15 nM, respectively. Eight % calcium-free fetal bovine serum rapidly caused a significant increase in [Ca2+]i, while causing both cell types to undergo squamous differentiation. When treated with 8% calcium-free fetal bovine serum, a serum-sensitive subclone of BEAS-2B cells exhibited a higher elevation of [Ca2+]i than a serum-resistant (i.e., not stimulated to differentiate by serum) subclone. However, a serum component involved in the induction of squamous differentiation, transforming growth factor type β, did not increase [Ca2+]i in either normal cells or BEAS-2B cells. 12-O-Tetradecanoylphorbol-13-acetate, an exogenous inducer of squamous differentiation and activator of protein kinase C, did not increase [Ca2+]i, but did attenuate serum-induced elevation of [Ca2+]i. These results suggest that while an increase in [Ca2+]i is associated with serum-induced squamous differentiation, a cytosolic ionized calcium signal is not required for the initiation of the squamous differentiation pathway induced by either transforming growth factor type β or 12-O-tetradecanoylphorbol-13-acetate.

Footnotes

  • ↵1 To whom requests for reprints should be addressed, at Building 37, Room 2C01, National Cancer Institute, Bethesda, MD 20892.

  • Received February 23, 1988.
  • Revision received August 18, 1988.
  • Accepted October 5, 1988.
  • ©1989 American Association for Cancer Research.
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January 1989
Volume 49, Issue 1
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Effects of Serum, Transforming Growth Factor Type β, or 12-O-Tetradecanoylphorbol-13-acetate on Ionized Cytosolic Calcium Concentration in Normal and Transformed Human Bronchial Epithelial Cells
Masao Miyashita, Mary W. Smith, James C. Willey, John F. Lechner, Benjamin F. Trump and Curtis C. Harris
Cancer Res January 1 1989 (49) (1) 63-67;

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Effects of Serum, Transforming Growth Factor Type β, or 12-O-Tetradecanoylphorbol-13-acetate on Ionized Cytosolic Calcium Concentration in Normal and Transformed Human Bronchial Epithelial Cells
Masao Miyashita, Mary W. Smith, James C. Willey, John F. Lechner, Benjamin F. Trump and Curtis C. Harris
Cancer Res January 1 1989 (49) (1) 63-67;
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