Abstract
Decarbazine induces DNA lesions in replicating DNA. We show here that deoxyribonucleosides, with the exception of thymidine, in doses of 250 µm or higher, prevent dacarbazine-induced DNA lesions. The DNA lesions that appear in the presence of thymidine can be prevented by aphidicolin, an inhibitor of DNA synthesis. Cytotoxicity analyses confirm that the combination thymidine-dacarbazine prevents cell outgrowth only partially, while other deoxyribonucleosides completely abolish the dacarbazine effect.
Footnotes
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↵1 This work was supported by grants from the Swedish Cancer Society and Karolinska Institutet.
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↵2 To whom requests for reprints should be addressed, at Radiumhemmet, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden.
- Received September 27, 1988.
- Revision received February 15, 1989.
- Accepted February 16, 1989.
- ©1989 American Association for Cancer Research.
Reduced DNA Damage Induced in Human Melanoma Cells by Dacarbazine in the Presence of Deoxyribonucleosides
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