Abstract
Decarbazine induces DNA lesions in replicating DNA. We show here that deoxyribonucleosides, with the exception of thymidine, in doses of 250 µm or higher, prevent dacarbazine-induced DNA lesions. The DNA lesions that appear in the presence of thymidine can be prevented by aphidicolin, an inhibitor of DNA synthesis. Cytotoxicity analyses confirm that the combination thymidine-dacarbazine prevents cell outgrowth only partially, while other deoxyribonucleosides completely abolish the dacarbazine effect.
Footnotes
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↵1 This work was supported by grants from the Swedish Cancer Society and Karolinska Institutet.
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↵2 To whom requests for reprints should be addressed, at Radiumhemmet, Karolinska Hospital, PO Box 60500, S-104 01 Stockholm, Sweden.
- Received September 27, 1988.
- Revision received February 15, 1989.
- Accepted February 16, 1989.
- ©1989 American Association for Cancer Research.
June 1989
Volume 49, Issue 11
Volume 49, Issue 11
Reduced DNA Damage Induced in Human Melanoma Cells by Dacarbazine in the Presence of Deoxyribonucleosides
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