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Altered Expression of Proteoglycans in E1A-immortalized Rat Fetal Intestinal Epithelial Cells in Culture

Peggy Lévy, Shahin Emami, Gisèle Cherqui, Eric Chastre, Christian Gespach and Jacques Picard
Peggy Lévy
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Shahin Emami
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Gisèle Cherqui
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Eric Chastre
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Christian Gespach
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Jacques Picard
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DOI:  Published October 1990
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Abstract

Normal and E1A-immortalized rat fetal intestinal epithelial SLC-11 cells were compared for the characteristics of the 35S-labeled proteoglycans isolated from their cell-associated and secreted fractions. In comparison with control cells in primary culture, immortalized SLC-11 cells: (a) secreted larger amounts of radiolabeled proteoglycans; (b) contained larger amounts of membrane-intercalated proteoglycans, analyzed by hydrophobic affinity chromatography on octyl-Sepharose; (c) produced cell-associated and secreted proteoglycans of smaller hydrodynamic size, assessed by measurement of Kav, values; (d) contained a higher percentage of heparan sulfate in the cell-associated proteoglycans, determined by differential susceptibility of glycosaminoglycans to specific glycosaminoglycan lyases; (e) displayed heparan sulfate and chondroitin sulfate with a shorter chain length; and (f) synthesized glycosaminoglycans with a lower degree of sulfation, determined by ion-exchange chromatography.

Taken together, these results demonstrate that in E1A-immortalized intestinal epithelial SLC-11 cells, the expression of proteoglycans alters considerably at an early stage of oncogenic transformation.

Footnotes

  • ↵1 Aided by a research grant from the Ligue contre le Cancer (1990).

  • Received January 12, 1990.
  • Accepted July 17, 1990.
  • ©1990 American Association for Cancer Research.
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October 1990
Volume 50, Issue 20
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Altered Expression of Proteoglycans in E1A-immortalized Rat Fetal Intestinal Epithelial Cells in Culture
Peggy Lévy, Shahin Emami, Gisèle Cherqui, Eric Chastre, Christian Gespach and Jacques Picard
Cancer Res October 15 1990 (50) (20) 6716-6722;

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Altered Expression of Proteoglycans in E1A-immortalized Rat Fetal Intestinal Epithelial Cells in Culture
Peggy Lévy, Shahin Emami, Gisèle Cherqui, Eric Chastre, Christian Gespach and Jacques Picard
Cancer Res October 15 1990 (50) (20) 6716-6722;
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