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Immunology

Expression of a Recombinant Breast Tumor-associated Mucin Fusion Protein in Escherichia coli Exposes the Tumor-specific Epitope

Peisheng Hu and Stephen E. Wright
Peisheng Hu
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Stephen E. Wright
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DOI:  Published October 1993
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Abstract

Mucins are highly immunogenic glycoproteins that are abundantly expressed by breast carcinomas and other carcinomas. The fact that deglycosylated normal mucin can induce tumor-specific monoclonal antibodies indicates that tumor-specific epitopes are hidden in the fully glycosylated form. Using recombinant DNA techniques, a fragment of mucin tandem repeats was inserted into pMal-p, an Escherichia coli expression vector, and resulted in the expression of an unglycosylated maltose-binding protein-mucin fusion protein. This fusion protein has been purified and showed strong affinity to breast tumor-specific monoclonal antibody SM3. The antisera against this recombinant mucin fusion protein recognized all breast tumor cell lines we tested. Competition assay with monoclonal antibody SM3 shows that anti-recombinant mucin fusion protein binds the epitope that SM3 binds. These results confirm the hypothesis that unglycosylated mucin contains a tumor-specific epitope. This leads to the possibility that recombinant mucin may be used to develop vaccines against breast cancer and cytotoxic T-lymphocyte lines for immunotherapy of breast cancer.

Footnotes

  • ↵1 This work was supported by Department of Veterans Affairs medical research funds to S. E. W. and Elsa U. Pardee Foundation to Dr. K. E. Dombrowski and S. E. W.

  • ↵3 To whom requests for reprints should be addressed, at Veterans Affairs Medical Center-151, 6010 Amarillo Boulevard West, Amarillo, TX 79106.

  • Received January 5, 1993.
  • Accepted August 11, 1993.
  • ©1993 American Association for Cancer Research.
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October 1993
Volume 53, Issue 20
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Expression of a Recombinant Breast Tumor-associated Mucin Fusion Protein in Escherichia coli Exposes the Tumor-specific Epitope
Peisheng Hu and Stephen E. Wright
Cancer Res October 15 1993 (53) (20) 4920-4926;

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Expression of a Recombinant Breast Tumor-associated Mucin Fusion Protein in Escherichia coli Exposes the Tumor-specific Epitope
Peisheng Hu and Stephen E. Wright
Cancer Res October 15 1993 (53) (20) 4920-4926;
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