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Tumor Biology

Decreased Connexin Expression and Intercellular Communication in Human Bladder Cancer Cells

H. Barton Grossman, Monica Liebert, Ike W. Lee and Sam W. Lee
H. Barton Grossman
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Monica Liebert
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Ike W. Lee
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Sam W. Lee
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DOI:  Published June 1994
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Abstract

Connexins make up a gene family encoding proteins that form intercellular channels known as gap junctions. Decreases in connexin expression and loss of intercellular communication have been associated with the malignant phenotype in some animal and human cells. The expression of connexin 26 and 43 mRNA was evaluated in cultured normal and malignant human urothelial cells. The normal urothelial cells were shown by Northern analysis to express both connexins. Increased confluence of the cultured normal human urothelial cells was associated with upregulation of connexin 26 mRNA. Connexin 26 mRNA expression was decreased in the bladder cancer cells. Using a human connexin 26 complementary DNA probe, nuclear run-on assays demonstrated that the decreased expression in the cancer cells was due to a failure of transcription. Southern blot analysis did not reveal any alterations in the genomic DNA. Assessment of gap junction function by scrape loading of lucifer yellow demonstrated dye transfer in normal urothelial cells but not in bladder cancer cells. Downregulation of connexin 26 mRNA was associated with functional loss of intercellular communication in the human bladder cancer cells. Connexin 43 expression varied considerably in the bladder cancer cell lines and did not correlate with dye transfer of lucifer yellow. These data suggest that alterations in the regulation of connexin 26 expression are associated with and may contribute to the malignant phenotype in bladder cancer.

Footnotes

  • ↵1 Supported in part by CA56973 from the National Cancer Institute, NIH, Department of Health and Human Services.

  • ↵2 To whom requests for reprints should be addressed, at 1500 E. Medical Center Drive, Ann Arbor, MI 48109-0330.

  • Received August 3, 1993.
  • Accepted April 5, 1994.
  • ©1994 American Association for Cancer Research.
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June 1994
Volume 54, Issue 11
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Decreased Connexin Expression and Intercellular Communication in Human Bladder Cancer Cells
H. Barton Grossman, Monica Liebert, Ike W. Lee and Sam W. Lee
Cancer Res June 1 1994 (54) (11) 3062-3065;

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Decreased Connexin Expression and Intercellular Communication in Human Bladder Cancer Cells
H. Barton Grossman, Monica Liebert, Ike W. Lee and Sam W. Lee
Cancer Res June 1 1994 (54) (11) 3062-3065;
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