Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Experimental Therapeutics

Estramustine-Binding Protein and Specific Binding of the Anti-Mitotic Compound Estramustine in Astrocytoma

A. Tommy Bergenheim, Per Björk, Jonas Bergh, Eva von Schoultz, Helena Svedberg and Roger Henriksson
A. Tommy Bergenheim
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Per Björk
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jonas Bergh
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eva von Schoultz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Helena Svedberg
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Roger Henriksson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published September 1994
  • Article
  • Info & Metrics
  • PDF
Loading

Abstract

Estramustine-binding protein (EMBP) is a Mr 46,000 heterodimeric protein originally isolated from prostatic tissue. It has a demonstrated high affinity for, and selective binding of, estramustine, which is a derivative of 17β-estradiol and nornitrogen mustard with antimitotic activity. In this study, we have analysed the expression of an EMBP-like protein in astrocytoma specimens. Immunohistochemistry revealed a pronounced reactivity for EMBP in astrocytoma grades III–IV as well as in metastatic prostatic adenocarcinoma used as positive control. In astrocytoma grades I–II, the expression was weak. The EMBP-like protein was quantified by radioimmunoassay in astrocytoma tumor tissue with higher concentrations in malignant astrocytoma, grades III–IV, compared to grades I–II tumors. Western immunoblotting of immunoaffinity purified EMBP-like protein under nonreducing conditions revealed an immunoreactivity corresponding to Mr 138,000 and 200,000, indicating a different structure of EMBP in astrocytoma compared to prostatic tissue. Specific binding and the presence of saturable binding sites for 3H-labeled estramustine were demonstrated in astrocytoma tissues expressing EMBP-like protein. Scatchard plot analysis showed a Kd at ≈30 nm, which suggests a binding affinity for estramustine in the same range as previously reported for EMBP in the prostate, Moreover, the number of estramustine binding sites/g tumor as calculated from the Scatchard plots was well correlated with the EMBP levels determined in the radioimmunoassay. In conclusion, an EMBP-like protein is expressed in astrocytoma. This protein may be responsible for the specific binding of estramustine in the tumor tissue. Whether this specific binding of estramustine is of importance for the cytotoxic effect in glioma cells remains to be evaluated.

Footnotes

  • ↵1 This investigation was supported by grants from The Swedish Cancer Society and The Lion Cancer Foundation, Umeå; Swedish Society for Medical Research, Karolinska Institutet, Stockholm; Umeå University; and Lundbergs Foundation for Medical Research, Gothenburg, Sweden.

  • ↵2 To whom requests for reprints should be addressed.

  • Received April 18, 1994.
  • Accepted July 20, 1994.
  • ©1994 American Association for Cancer Research.
PreviousNext
Back to top
September 1994
Volume 54, Issue 18
  • Table of Contents
  • Table of Contents (PDF)
  • Index by Author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)

Sign up for alerts

Open full page PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Estramustine-Binding Protein and Specific Binding of the Anti-Mitotic Compound Estramustine in Astrocytoma
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Estramustine-Binding Protein and Specific Binding of the Anti-Mitotic Compound Estramustine in Astrocytoma
A. Tommy Bergenheim, Per Björk, Jonas Bergh, Eva von Schoultz, Helena Svedberg and Roger Henriksson
Cancer Res September 15 1994 (54) (18) 4974-4979;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Estramustine-Binding Protein and Specific Binding of the Anti-Mitotic Compound Estramustine in Astrocytoma
A. Tommy Bergenheim, Per Björk, Jonas Bergh, Eva von Schoultz, Helena Svedberg and Roger Henriksson
Cancer Res September 15 1994 (54) (18) 4974-4979;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • PDF
Advertisement

Related Articles

Cited By...

More in this TOC Section

Experimental Therapeutics

  • Novel Mechanisms of Apoptosis Induced by Histone Deacetylase Inhibitors
  • Phosphatidylinositol 3′-Kinase Is Required for Growth of Mast Cells Expressing the Kit Catalytic Domain Mutant
  • Antitumor Effect by Interleukin-11 Receptor α-Locus Chemokine/CCL27, Introduced into Tumor Cells through a Recombinant Adenovirus Vector
Show more 3

Articles

  • Laureates
  • Identification and Characterization of Collaborating Oncogenes in Compound Mutant Mice
  • The Role of Chimeric Paired Box Transcription Factors in the Pathogenesis of Pediatric Rhabdomyosarcoma
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement