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Molecular Biology and Genetics

Allelotyping of Ductal Carcinoma in Situ of the Breast: Deletion of Loci on 8p, 13q, 16q, 17p and 17q

Diane M. Radford, Keri L. Fair, Nancy J. Phillips, Jon H. Ritter, Todd Steinbrueck, Matthew S. Holt and Helen Donis-Keller
Diane M. Radford
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Keri L. Fair
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Nancy J. Phillips
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Jon H. Ritter
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Todd Steinbrueck
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Matthew S. Holt
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Helen Donis-Keller
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DOI:  Published August 1995
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Abstract

In order to determine which tumor suppressor loci are involved in preinvasive breast cancer, we have assayed for loss of heterozygosity (LOH) in ductal carcinoma in situ (DCIS). Areas of DCIS were microdissected from archival paraffin-embedded tissue. DNA was extracted, and LOH was determined by PCR of microsatellite markers that map to 39 autosomal arms. Either uninvolved lymph node or white cell DNA was used as normal control. A total of 61 samples of DCIS were assayed. The average number of informative tumors examined for each marker was 19 (range, 8–48). The median fractional allelic loss was 0.037. The highest percentage of LOH was shown for loci on 8p (18.7%), 13q (18%), 16q (28.6%), 17p (37.5%), and 17q (15.9%). LOH on 18q was found in 10.7% of informative tumors. Fractional allelic loss was associated with LOH on 17p, with high nuclear grade and with the comedo subtype of DCIS. LOH on 17p correlated with LOH on 17q and on 13q. Additional markers were used for 16q and 17p to determine the smallest common region of deletion. These data provide evidence that tumor suppressor loci that map to these regions are involved in the oncogenesis of breast cancer before progression to the invasive phenotype. Our findings provide additional support that multiple loci on 17p and 16q are involved in the development of breast cancer.

Footnotes

  • ↵1 Supported by a Veterans Administration RAG grant and an American Cancer Society Career Development award (D.M.R.) and in part by NIH Grants HG00469 and HG01066-01A1 (H.D.K.).

  • ↵2 To whom requests for reprints should be addressed, at 9901 Wohl Hospital, 660 S. Euclid Avenue, St. Louis, MO 63110.

  • Received January 31, 1995.
  • Accepted May 31, 1995.
  • ©1995 American Association for Cancer Research.
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August 1995
Volume 55, Issue 15
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Allelotyping of Ductal Carcinoma in Situ of the Breast: Deletion of Loci on 8p, 13q, 16q, 17p and 17q
Diane M. Radford, Keri L. Fair, Nancy J. Phillips, Jon H. Ritter, Todd Steinbrueck, Matthew S. Holt and Helen Donis-Keller
Cancer Res August 1 1995 (55) (15) 3399-3405;

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Allelotyping of Ductal Carcinoma in Situ of the Breast: Deletion of Loci on 8p, 13q, 16q, 17p and 17q
Diane M. Radford, Keri L. Fair, Nancy J. Phillips, Jon H. Ritter, Todd Steinbrueck, Matthew S. Holt and Helen Donis-Keller
Cancer Res August 1 1995 (55) (15) 3399-3405;
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