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Molecular Biology and Genetics

Accurate and Objective Preoperative Diagnosis of Thyroid Papillary Carcinomas by Reverse Transcription-PCR Detection of Oncofetal Fibronectin Messenger RNA in Fine-Needle Aspiration Biopsies

Toru Takano, Akira Miyauchi, Tamotsu Yokozawa, Fumio Matsuzuka, Gang Liu, Takuya Higashiyama, Shinji Morita, Kanji Kuma and Nobuyuki Amino
Toru Takano
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Akira Miyauchi
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Tamotsu Yokozawa
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Fumio Matsuzuka
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Gang Liu
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Takuya Higashiyama
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Shinji Morita
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Kanji Kuma
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Nobuyuki Amino
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DOI:  Published November 1998
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Abstract

Recently, the restricted expression of oncofetal fibronectin mRNA was reported in thyroid papillary and anaplastic carcinomas. In this study, by extracting RNA from the leftover cells inside the needles used for fine-needle aspiration biopsy, we establish a new method for gene diagnosis of these carcinomas without further invasiveness to the patient (aspiration biopsy-reverse transcription-PCR, ABRP). RNA was extracted from 177 fine-needle aspiration biopsies of thyroid nodules that were suspicious for malignancy, and then the gene diagnoses made by reverse transciption-PCR detection of oncofetal fibronectin mRNA were compared with cytological diagnoses. Thirty-five (94.6%) of 37 samples that were diagnosed as papillary or anaplastic carcinomas by cytological examination showed a positive result by gene diagnosis, whereas only 4 (3.7%) of 109 samples that were cytologically diagnosed negative for both carcinomas showed a positive result. Among all of the cases, 50 patients underwent surgery, and a histological diagnosis was consequently made. The sensitivity and specificity of this method were 96.9 and 100%, respectively. A combined examination using both genetic and cytological approaches may contribute to a more precise preoperative diagnosis of papillary and anaplastic carcinomas.

Footnotes

  • ↵1 This work was supported by a Grant-in-Aid 10771346 for Encouragement of Young Scientists (to T. T.) from the Ministry of Education, Science, Sports and Culture of Japan and Grants-in-Aid from Osaka Cancer Research Foundation and Clinical Pathology Research Foundation of Japan.

  • ↵2 To whom requests for reprints should be addressed, Department of Laboratory Medicine, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

  • Received April 2, 1998.
  • Accepted August 31, 1998.
  • ©1998 American Association for Cancer Research.
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November 1998
Volume 58, Issue 21
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Accurate and Objective Preoperative Diagnosis of Thyroid Papillary Carcinomas by Reverse Transcription-PCR Detection of Oncofetal Fibronectin Messenger RNA in Fine-Needle Aspiration Biopsies
Toru Takano, Akira Miyauchi, Tamotsu Yokozawa, Fumio Matsuzuka, Gang Liu, Takuya Higashiyama, Shinji Morita, Kanji Kuma and Nobuyuki Amino
Cancer Res November 1 1998 (58) (21) 4913-4917;

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Accurate and Objective Preoperative Diagnosis of Thyroid Papillary Carcinomas by Reverse Transcription-PCR Detection of Oncofetal Fibronectin Messenger RNA in Fine-Needle Aspiration Biopsies
Toru Takano, Akira Miyauchi, Tamotsu Yokozawa, Fumio Matsuzuka, Gang Liu, Takuya Higashiyama, Shinji Morita, Kanji Kuma and Nobuyuki Amino
Cancer Res November 1 1998 (58) (21) 4913-4917;
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