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Molecular Biology and Genetics

Frequent Deletion and 5′ CpG Island Methylation of the p16 Gene in Primary Malignant Lymphoma of the Brain

Shu-Jing Zhang, Sumio Endo, Tomio Ichikawa, Kazuo Washiyama and Toshiro Kumanishi
Shu-Jing Zhang
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Sumio Endo
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Tomio Ichikawa
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Kazuo Washiyama
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Toshiro Kumanishi
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DOI:  Published March 1998
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Abstract

A total of 10 primary malignant lymphomas of the brain were examined for deletion, mutation, and 5′ CpG island methylation of the p16 gene, which is a candidate tumor suppressor gene with CDK-inhibitory function. In Southern blot analysis, p16 gene deletion was suggested in nine cases, homozygously (five cases) or hemizygously (four cases). In the remaining one case, p16 gene deletion was not suggested. Although single-strand conformation polymorphism and nucleotide analyses suggested no mutations of the p16 gene in these cases, methylation analyses revealed 5′ CpG island methylation in three cases, of which two were those with presumed hemizygous deletion and one was that without deletion in Southern blot analysis. Thus, p16 gene abnormality was detected in all 10 of the brain lymphomas examined, and in 8 of them, actual p16 gene inactivation was suggested by their homozygous deletion (5 cases) or 5′ CpG island methylation (3 cases). These findings suggest that p16 gene abnormality and inactivation are closely related to carcinogenesis in primary malignant lymphoma of the brain. The p15 gene, another candidate tumor suppressor gene located in the vicinity of the p16 gene, to which it shows structural and functional similarity, was also presumed to be deleted similarly in most cases. Its methylation was seen in one case, the case without the methylated p16 gene.

Footnotes

  • ↵1 This work was supported by grants from the Yoneyama Foundation, the Association of International Education, Japan, the Inoue Foundation for Science, the Fujisawa Foundation, the Sasakawa Health, Science Foundation, and the Ministry of Education, Science, Sports and Culture of Japan.

  • ↵2 To whom requests for reprints should be addressed. Phone: 81-25-223-6161, ext. 5141; Fax: 81-25-229-2959.

  • Received June 6, 1997.
  • Accepted January 12, 1998.
  • ©1998 American Association for Cancer Research.
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March 1998
Volume 58, Issue 6
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Frequent Deletion and 5′ CpG Island Methylation of the p16 Gene in Primary Malignant Lymphoma of the Brain
Shu-Jing Zhang, Sumio Endo, Tomio Ichikawa, Kazuo Washiyama and Toshiro Kumanishi
Cancer Res March 15 1998 (58) (6) 1231-1237;

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Frequent Deletion and 5′ CpG Island Methylation of the p16 Gene in Primary Malignant Lymphoma of the Brain
Shu-Jing Zhang, Sumio Endo, Tomio Ichikawa, Kazuo Washiyama and Toshiro Kumanishi
Cancer Res March 15 1998 (58) (6) 1231-1237;
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Cancer Research Online ISSN: 1538-7445
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