Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Immunology/Tumor Immunobiology 3: Specific Immunomechanisms and Cancer Vaccines: Basic Studies I

Immunization targeting a cell surface glycoantigen mediates apoptosis of tumor cells

Behjatolah Monzavi Karbassi, Cecile Artaud, Fariba Jousheghany, Saeid Shaaf, Soheila Korourian, Joseph S. Stanley and Thomas Kieber-Emmons
Behjatolah Monzavi Karbassi
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cecile Artaud
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Fariba Jousheghany
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Saeid Shaaf
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Soheila Korourian
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joseph S. Stanley
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas Kieber-Emmons
Arkansas Cancer Research Center and Department of Pathology, Univ of Arkansas for Medical Sciences, Little Rock, AR
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published April 2004
  • Article
  • Info & Metrics
Loading
Proc Amer Assoc Cancer Res, Volume 45, 2004

Abstract

1282

Introduction. Carbohydrate-reactive lectins have shown an ability to mediate apoptosis upon binding of tumor cells. Induction of humoral responses that mediate apoptosis is perceived to be beneficial in vaccine formulations. Here we show that immunization with a DNA form of a rationally chosen peptide mimotope of lectin-reactive tumor-associated carbohydrate antigen induces a humoral response that mediates apoptosis and significantly increases survival rate of tumor-bearing animal in metastatic murine mammary 4T1 tumor model. Experimental procedures. Flow cytometry was utilized to profile 4T1 cell surface carbohydrates using plant lectins and for detection of apoptosis. An array of carbohydrate mimotopes was screened with the lectins by ELISA assay. Peptides were translated to DNA and cloned into a plasmid that is designed to secret the expressed product. Mice were inoculated with 4T1 cells in the abdominal mammary gland. DNA immunization started 4 days post-tumor transplant and each mouse received three intramuscular injection of each construct. Tumor size was measured regularly and survival date recorded. Lectin histochemistry was performed using organs harvested from tumor-bearing mice. Metastasis was assessed by growth in selective medium. Results. We observed that Griffonia simplicifolia lectin I (GS-I) binds to 4T1 cells. A peptide mimotope that was chosen based on its reactivity with GS-I inhibits the binding of the lectin to 4T1 cells, indicating that the peptide mimics a carbohydrate structure reactive with GS-I. By analysis of annexin V binding we observed that GS-I induces apoptosis in 4T1 cells. Immunization with the peptide mimic generates antibodies that bind to the cells and induce apoptosis in tumor cells. We further observed that therapeutic immunization of tumor-bearing animals with the peptide-encoding construct results in slight tumor shrinkage but statistically significant (P = 0.021) higher survival rate as compared with vector immunized animals. Immunization with the peptide significantly diminished metastasis to the liver (P= 0.018). Histochemistry demonstrates that all tumor cells in liver are stained with GS-I, while staining is partial for tumor cells in the primary mass and lung. Histochemical data imply that liver metastasis might depend on the presence of GS-I-reactive structure on migrating cells. Conclusion. Here we report that a peptide surrogate of a tumor associated carbohydrate antigen can trigger an in vivo tumor-specific response in which serum antibodies may function as mediators of cell apoptosis. Induction of apoptosis in combination with activation of the immune response might be an effective strategy for vaccination against weakly immunogenic malignancies.

  • American Association for Cancer Research
Previous
Back to top
April 2004
Volume 64, Issue 7 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Immunization targeting a cell surface glycoantigen mediates apoptosis of tumor cells
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Immunization targeting a cell surface glycoantigen mediates apoptosis of tumor cells
Behjatolah Monzavi Karbassi, Cecile Artaud, Fariba Jousheghany, Saeid Shaaf, Soheila Korourian, Joseph S. Stanley and Thomas Kieber-Emmons
Cancer Res April 1 2004 (64) (7 Supplement) 293-294;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Immunization targeting a cell surface glycoantigen mediates apoptosis of tumor cells
Behjatolah Monzavi Karbassi, Cecile Artaud, Fariba Jousheghany, Saeid Shaaf, Soheila Korourian, Joseph S. Stanley and Thomas Kieber-Emmons
Cancer Res April 1 2004 (64) (7 Supplement) 293-294;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Phagocytosis of damaged 9L glioma cells by microglia/macrophages isolated from adult rat brains
  • Identification of DHBcAg as a potent carrier protein comparable to KLH for augmenting MUC1 antigenicity
Show more Immunology/Tumor Immunobiology 3: Specific Immunomechanisms and Cancer Vaccines: Basic Studies I
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement