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Cellular, Molecular, and Tumor Biology 46: Signaling and Tumor Invasion

Characterization of p31CTN, a new α-catenin binding protein.

Gerald W. Verhaegh, Marianne Linkels, Kjeld P. Van Houwelingen, Egbert Oosterwijk and Jack A. Schalken
Gerald W. Verhaegh
University Medical Centre Nijmegen, Nijmegen, Netherlands.
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Marianne Linkels
University Medical Centre Nijmegen, Nijmegen, Netherlands.
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Kjeld P. Van Houwelingen
University Medical Centre Nijmegen, Nijmegen, Netherlands.
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Egbert Oosterwijk
University Medical Centre Nijmegen, Nijmegen, Netherlands.
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Jack A. Schalken
University Medical Centre Nijmegen, Nijmegen, Netherlands.
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DOI:  Published April 2004
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Proc Amer Assoc Cancer Res, Volume 45, 2004

Abstract

2436

Alpha-catenin plays a crucial role in E-cadherin mediated cell-cell adhesion by coupling the E-cadherin-beta/gamma-catenin complex to the actin cytoskeleton of the cell. Absence or dysfunction of α-catenin leads to an impaired E-cadherin function, and a concomitant loss of epithelial integrity. In addition to the direct binding of α-catenin to α-actinin and actin filaments, other proteins might be involved in the binding to, and signaling via the cytoskeleton. By screening a yeast two hybrid cDNA library using full length α-catenin as a bait, a gene, named p31CTN, was identified that encodes a 31kDa protein that binds to α-catenin. No sequence homology was found with any protein described so far. To confirm that p31CTN is a bona fide interactant of α-catenin, co-localization and co-immunoprecipitation were performed using stable p31CTN transfectants in the human A431 cell line. Furthermore, the effect of ectopic overexpression of p31CTN on cell migration was studied. Co-immunoprecipitation experiments demonstrated that p31CTN interacts with α-catenin and the other proteins of the E-cadherin/catenin complex. Furthermore, the p31CTN protein co-localizes with E-cadherin and α, β and γ-catenin to the cell membrane. Overexpression of p31CTN resulted in an increased cell migration as determined by a scratch assay, indicating that p31CTN destabilizes or modulates the E-cadherin/catenin-mediated cell adhesion complex. Interestingly, although p31CTN was found to be ubiquitously expressed in normal human tissues, a significant upregulation was found in several tumor specimens. In conclusion, p31CTN is a novel protein that physically interacts with α-catenin. Increased expression of p31CTN was observed in tumor cells, and in vitro led to an increased cell migration. These data suggest that p31CTN might be a novel tumor invasion marker.

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April 2004
Volume 64, Issue 7 Supplement
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Characterization of p31CTN, a new α-catenin binding protein.
Gerald W. Verhaegh, Marianne Linkels, Kjeld P. Van Houwelingen, Egbert Oosterwijk and Jack A. Schalken
Cancer Res April 1 2004 (64) (7 Supplement) 564;

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Characterization of p31CTN, a new α-catenin binding protein.
Gerald W. Verhaegh, Marianne Linkels, Kjeld P. Van Houwelingen, Egbert Oosterwijk and Jack A. Schalken
Cancer Res April 1 2004 (64) (7 Supplement) 564;
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Cancer Research Online ISSN: 1538-7445
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