Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Clinical Research 2: Molecular Diagnosis and Prognosis I

Overexpression of tuberous sclerosis complex 2 exerts antitumor effect on oral cancer cells

Shin-Ichi Kawaguchi, Koji Harada, Supri Atno, Hideo Yoshida and Mitsunobu Sato
Shin-Ichi Kawaguchi
Department of Oral and Maxillofacial Radiology and Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, Tokushima, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Koji Harada
Department of Oral and Maxillofacial Radiology and Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, Tokushima, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Supri Atno
Department of Oral and Maxillofacial Radiology and Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, Tokushima, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hideo Yoshida
Department of Oral and Maxillofacial Radiology and Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, Tokushima, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mitsunobu Sato
Department of Oral and Maxillofacial Radiology and Second Department of Oral and Maxillofacial Surgery, University of Tokushima, School of Dentistry, Tokushima, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published April 2004
  • Article
  • Info & Metrics
Loading
Proc Amer Assoc Cancer Res, Volume 45, 2004

Abstract

410

Tuberous sclerosis is caused by mutations in tuberous sclerosis complex (TSC) 2 on chromosome 16p13.3, encoding tuberin which is thought to be essential for p27Kip1 to regulate the cell cycle. In this study, we conducted to examine whether overexpression of TSC2 can affect the growth of oral cancer cells which have different expression level of p27Kip1 protein and also investigated whether tuberin expression can be an useful prognostic factor in oral cancer patients. We constructed an expression vector containing sense-oriented rat TSC2 cDNA with pcDNA3.1. We transfected oral cancer cells, B88t (high expression of p27Kip1 protein) and HI (low expression of p27Kip1 protein) with the sense expression vector to up-regulate the expression of TSC2 gene. Moreover, tuberin and p27Kip1 expression was investigated by immunohistochemistry in tissue samples from 100 patients with oral cancer. Associations of tuberin expression with clinicopathological characteristics and patient survival were also analyzed. Overexpression of TSC2 exerted the growth inhibitory effect of B88t and HI in vitro and also exerted the antitumor effect on oral cancer cells whether they have high expression of p27Kip1 protein or not in vivo. In addition, there was significant association found between tuberin expression and therapeutic effect (p<0.01), patient outcome (p<0.01), the 5-year-survival rates(p<0.01). In general, loss of p27Kip1 thought to be associated with disease progression and an unfavorable outcome in several malignancies including an oral cancer. But, these findings suggest that overexpression of TSC2 may exert the antitumor effect on oral cancer cells whether they have high expression of p27Kip1 protein or not, and expression of tuberin may play an important role in the progression of oral cancer and is considered to be a useful prognostic factor in oral cancer patients.

  • American Association for Cancer Research
Previous
Back to top
April 2004
Volume 64, Issue 7 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Overexpression of tuberous sclerosis complex 2 exerts antitumor effect on oral cancer cells
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Overexpression of tuberous sclerosis complex 2 exerts antitumor effect on oral cancer cells
Shin-Ichi Kawaguchi, Koji Harada, Supri Atno, Hideo Yoshida and Mitsunobu Sato
Cancer Res April 1 2004 (64) (7 Supplement) 93;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Overexpression of tuberous sclerosis complex 2 exerts antitumor effect on oral cancer cells
Shin-Ichi Kawaguchi, Koji Harada, Supri Atno, Hideo Yoshida and Mitsunobu Sato
Cancer Res April 1 2004 (64) (7 Supplement) 93;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Stromelysin-2 (MMP-10) is overexpressed and enzymatically active in human lung cancers
  • Simultaneous detection and relative quantification of leukemia-associated chromosome translocation fusion genes using a combined multiplex RT-PCR and fluorescent bead detection system
  • Identification of genes for detection of circulating tumor cells in blood using real-time RT-PCR
Show more Clinical Research 2: Molecular Diagnosis and Prognosis I
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement