Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Experimental and Molecular Therapeutics 29: Nonviral Gene Therapy Approaches to Cancer

Interleukin-10 plasmid DNA inhibits liver and lung metastasis of Colon26 adenocarcinoma in mice

Takeshi Ishikawa, Satoshi Kokura, Hiroshi Higashihara, Naoyuki Sakamoto, Nami Nakabe, Haruki Kato, Tetsuro Yamane, Yuji Naito, Norimasa Yoshida and Toshikazu Yoshikawa
Takeshi Ishikawa
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Satoshi Kokura
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Hiroshi Higashihara
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Naoyuki Sakamoto
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nami Nakabe
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haruki Kato
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsuro Yamane
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yuji Naito
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norimasa Yoshida
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshikazu Yoshikawa
Matsushita Memorial Hospital, Moriguchi Osaka, Japan, Biomedical Safety Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Inflammation and Immunology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan, Molecular Gastroenterology and Hepatology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto Japan, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published May 2005
  • Article
  • Info & Metrics
Loading
Proc Amer Assoc Cancer Res, Volume 46, 2005

Abstract

3364

We demonstrated that Interluekin-10 (IL-10) gene therapy markedly inhibited liver and lung metastasis of Colon26 adenocarcinoma. The transcription factor NF-kB is constitutively activated in many human cancers, and induces the expression of multiple genes including adhesion molecules, COX-2, and VEGF. Therefore, the blocking of NF-kB activity may be associated with the suppression of angiogenesis, invasion, and metastasis. Recent papers indicate that NF-kB activation is inhibited by IL-10. Accordingly, we hypothesized that systemic IL-10 treatment could induce the blocking of NF-kB activation, resulting in the inhibition of the expression of genes that mediate metastasis. To induce a high level of IL-10 in plasma, we transferred the naked EBV-derived plasmid vectors encoding the human IL-10 gene (pGEG.IL-10) into the liver via the intravenous route. A single injection of pGEG.IL-10 elevated IL-10 in the plasma of mice with the peak levels (115 pg/mL) observed on the second day. An injection of additional pGEG.IL-10 five days later significantly increased the plasma level of IL-10 again. This high level of IL-10 was maintained by readministering pGEG.IL-10. The inoculation of Colon26 carcinoma cells into mice caused the activation of NF-kB, resulting in a marked increase in the gene expression of TNFα, COX-2, and VEGF in the liver. In contrast, pGEG.IL-10 inhibited the activation of NF-kB in the liver. pGEG.IL-10 also inhibited liver and lung metastasis of Colon26, possibly via a decrease in the transactivation of important NF-kB-driven genes necessary for the processes of cancer metastasis and growth. Our gene-transfer technique induced the IL-10 gene transfection primarily in hepatocytes, and concentrations of IL-10 in the liver were consequently high. The protocol induces the desired inhibition of liver metastasis, but an IL-10 concentration 115 pg/mL, the plasma level induced by the IL-10 gene transfection, is also sufficient to inhibit the lung metastases of colon cancer. These results demonstrate that the activation of NF-kB in response to an inoculation of carcinoma cells is an important mechanism of metastasis, and that systemic IL-10 gene therapy could be a new therapeutic strategy for cancer metastasis.

  • American Association for Cancer Research
Previous
Back to top
Cancer Research: 65 (9 Supplement)
May 2005
Volume 65, Issue 9 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Interleukin-10 plasmid DNA inhibits liver and lung metastasis of Colon26 adenocarcinoma in mice
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Interleukin-10 plasmid DNA inhibits liver and lung metastasis of Colon26 adenocarcinoma in mice
Takeshi Ishikawa, Satoshi Kokura, Hiroshi Higashihara, Naoyuki Sakamoto, Nami Nakabe, Haruki Kato, Tetsuro Yamane, Yuji Naito, Norimasa Yoshida and Toshikazu Yoshikawa
Cancer Res May 1 2005 (65) (9 Supplement) 792-793;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Interleukin-10 plasmid DNA inhibits liver and lung metastasis of Colon26 adenocarcinoma in mice
Takeshi Ishikawa, Satoshi Kokura, Hiroshi Higashihara, Naoyuki Sakamoto, Nami Nakabe, Haruki Kato, Tetsuro Yamane, Yuji Naito, Norimasa Yoshida and Toshikazu Yoshikawa
Cancer Res May 1 2005 (65) (9 Supplement) 792-793;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Prolonged half-life and limited decrease in dGTP results in additive rather than synergistic bystander cytotoxicity in SW480 cells treated with ganciclovir and hydroxyurea
  • HRE copy number affects hypoxia-induced BAX gene expression and function in human glioblastoma cells
  • Development of specific gene therapy by bio-compatible MPC polymer combined with hepatitis B surface antigen
Show more Experimental and Molecular Therapeutics 29: Nonviral Gene Therapy Approaches to Cancer
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement