Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Experimental and Molecular Therapeutics 22: Adenovector-based Therapy

Bifunctional therapeutic effects on peritoneal cell layer and cancer cells in calponin h1 gene therapy to prevent peritoneal dissemination of ovarian cancer

Hiroaki Kobayashi, Tomonori Ogura, Yousuke Ueoka, Kaoru Okugawa, Kiyoko Kato, Toshio Hirakawa, Norio Wake, Shigenari Hashimoto and Shun’ichiro Taniguchi
Hiroaki Kobayashi
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tomonori Ogura
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Yousuke Ueoka
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaoru Okugawa
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiyoko Kato
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshio Hirakawa
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Norio Wake
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shigenari Hashimoto
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shun’ichiro Taniguchi
Kyushu University, Fukuoka, Japan and Shinshu University, Matsumoto, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published April 2006
  • Article
  • Info & Metrics
Loading
Proc Amer Assoc Cancer Res, Volume 47, 2006

Abstract

2982

Calponin h1 (CNh1), one of the family of actin-binding proteins, stabilizes the filaments of actin and modulates various cellular biological phenotypes. We have reported that ovarian cancer cells secrete factors and probably make favorable environment for their invasion through the down-regulation of CNh1 and alpha-smooth muscle actin (α-SMA) in host stromal cells, such as peritoneal mesothelial cells, fibroblasts and cells forming blood vessel walls. In this study, we investigated the effects of CNh1 gene-transfection into peritoneal cells and ovarian cancer cells, and the efficacy of intraperitoneal (i.p.) CNh1 gene therapy against peritoneal dissemination of ovarian cancer. Adenoviral vector-mediated CNh1 gene transfections into peritoneal cells and ovarian cancer cells induced the stable formation of longer and thicker actin stress fibers. The infected peritoneal cells acquired an ability to resist intercellular dissociation induced by the conditioned medium of ovarian cancer cells, and thus cancer cell invasion through the monolayer of peritoneal cells was inhibited. In addition, CNh1-transfected ovarian cancer cells showed retarded growth property and invasiveness, the latter of which accompanied impaired cell motility. The concomitant CNh1 transfection into both the peritoneal cells and ovarian cancer cells produced an additive inhibitory effect with respect to cancer cell invasion through the peritoneal cell monolayer. In in vivo experiments designed to treat nude mice i.p. inoculated with ovarian cancer cells, we found that the i.p. injected CNh1-adenovirus successfully blocked ovarian cancer-induced morphological changes in peritoneal cell surface, and significantly prolonged the survival time of tumor-bearing mice without any serious side effects. Thus CNh1 gene therapy against peritoneal dissemination of ovarian cancer is bifunctionally effective, i.e. through inhibitory effects on the infected peritoneal cell layers which suppress cancer invasion and, second, through direct anti-tumor effect on invasion and growth properties of cancer cells. As such, this therapy may be considered as a potentially novel therapeutic intervention, whereby one and the same gene has two distinctive effects: one to control the cancer cells and the other to bolster a host defense (anti-invasive) mechanism.

  • American Association for Cancer Research
Previous
Back to top
Cancer Research: 66 (8 Supplement)
April 2006
Volume 66, Issue 8 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Bifunctional therapeutic effects on peritoneal cell layer and cancer cells in calponin h1 gene therapy to prevent peritoneal dissemination of ovarian cancer
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Bifunctional therapeutic effects on peritoneal cell layer and cancer cells in calponin h1 gene therapy to prevent peritoneal dissemination of ovarian cancer
Hiroaki Kobayashi, Tomonori Ogura, Yousuke Ueoka, Kaoru Okugawa, Kiyoko Kato, Toshio Hirakawa, Norio Wake, Shigenari Hashimoto and Shun’ichiro Taniguchi
Cancer Res April 15 2006 (66) (8 Supplement) 702;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Bifunctional therapeutic effects on peritoneal cell layer and cancer cells in calponin h1 gene therapy to prevent peritoneal dissemination of ovarian cancer
Hiroaki Kobayashi, Tomonori Ogura, Yousuke Ueoka, Kaoru Okugawa, Kiyoko Kato, Toshio Hirakawa, Norio Wake, Shigenari Hashimoto and Shun’ichiro Taniguchi
Cancer Res April 15 2006 (66) (8 Supplement) 702;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

  • Exportin 1-mediated nuclear export of adenovirus E1A protein: a novel target for generation of oncolytic viruses
  • Optimal strategy for loco-regional delivery of adenoviral vectors
  • Tumor-specific replicating Ad in combination with chemotherapy elicit synergistic anti-tumor effect in head & neck squamous cell carcinoma
Show more Experimental and Molecular Therapeutics 22: Adenovector-based Therapy
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement