Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Experimental and Molecular Therapeutics

Autophagy precedes caspase-independent cell death in chemo-resistant ovarian cancer cells

Ayesha Alvero, Michele Montagna, Ki Hyung Kim and Gil Mor
Ayesha Alvero
Yale Univ. School of Medicine, New Haven, CT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Michele Montagna
Yale Univ. School of Medicine, New Haven, CT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ki Hyung Kim
Yale Univ. School of Medicine, New Haven, CT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gil Mor
Yale Univ. School of Medicine, New Haven, CT
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI:  Published May 2007
  • Article
  • Info & Metrics
Loading
AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

Abstract

3216

Introduction: The role of autophagy in cancer is controversial. Initially autophagy was thought to represent an alternative pathway for cell death. However, more recently it has been suggested to play a role in tumor survival. In this study we describe how the induction of autophagy by a novel isoflavone derivative, NV128, induce cell death in chemo-resistant ovarian cancer cells. Methods: Eight primary cultures and two established epithelial ovarian cancer (EOC) cell lines were treated with increasing concentrations of NV128 (0.1, 1, and 10 ug/ml) with or without the pan-caspase inhibitor, Z-VAD-FMK. Cell viability was determined after 24h using the Celltiter 96 assay. DNA fragmentation was analyzed by flow cytometry with Hoechst and Propidium iodide staining. Activity of caspases- 3/7, -8, and -9 was measured using Caspase-Glo assay. Autophagic markers LC3-II and Beclin-1 was determined by Western blot analysis. Results: NV128 treatment decreased cell viability in all tested EOC cells lines in a dose-dependent manner with IC50 between 1 and 5 ug/ml. Flow cytometry analysis revealed DNA fragmentation, with Hoechst and Propidium iodide double-positive cells increasing 2-4 fold after NV128 treatment. However, cell death was caspase independent as evidenced by the lack of caspases- 3/7, -8, and -9 activity and the inability of the caspase inhibitor, Z-VAD-FMK, to prevent cell death. Western blot analysis of autophagic markers showed early involvement of LC3-II and Beclin-1. Conclusion: We demonstrate that induction of autophagy by NV128 in chemo-resistant ovarian cancer cells is able to promote caspase- independent cell death. Our results suggest that the autophagic pathway can be an alternative target in EOC cells, especially those that are highly resistant to apoptosis.

Footnotes

  • 98th AACR Annual Meeting-- Apr 14-18, 2007; Los Angeles, CA

  • American Association for Cancer Research
Previous
Back to top
Cancer Research: 67 (9 Supplement)
May 2007
Volume 67, Issue 9 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Autophagy precedes caspase-independent cell death in chemo-resistant ovarian cancer cells
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Autophagy precedes caspase-independent cell death in chemo-resistant ovarian cancer cells
Ayesha Alvero, Michele Montagna, Ki Hyung Kim and Gil Mor
Cancer Res May 1 2007 (67) (9 Supplement) 3216;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Autophagy precedes caspase-independent cell death in chemo-resistant ovarian cancer cells
Ayesha Alvero, Michele Montagna, Ki Hyung Kim and Gil Mor
Cancer Res May 1 2007 (67) (9 Supplement) 3216;
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Experimental and Molecular Therapeutics

  • Abstract SY14-03: Anaplastic lymphoma kinase (ALK): Normal biology and role in hematopoietic malignancies
  • Abstract SY18-01: Targeting the p53-MDM2 interaction for cancer therapy
  • Abstract SSY01-04: Discovery and validation of genome-wide genetic signatures of chemotherapy susceptibility: A translational model
Show more 3

Cell Cycle and Apoptosis: Poster Presentations

  • Near-infrared Raman microspectroscopic analysis of the effect of etoposide on C6 glioma cell division cycle
  • Role of MAP-kinase pathways in photodynamic therapy: Mediated apoptosis in human multidrug resistant uterine sarcoma cells
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement