Abstract
Retinoid derivatives have been used for chemoprevention for ovarian cancer. However, the molecular mechanisms are not yet fully understood. PTEN, a tumor suppressor gene and a PI3K functional antagonist, has been evaluated in cancer cell proliferation, migration and drug resistance. To determine if retinoids have an effect on PTEN-linked signaling during tumor suppression in ovarian cancer, we examined the ovarian epithelium cells (IOSE) and ovarian cancer cells (SKOV3 and Hey) response to retinoids in the scratch wound healing assay. The results showed that all-trans retinoic acid (ATRA) inhibited healing response in IOSE and SKOV3 cells but not in Hey cells. In addition, Western blotting showed that RA increased PTEN expression and phosphorylation of PTEN in IOSE cells but decreased the level of phosphorylated PTEN in SKOV3 and Hey cells which was confirmed with cDNA arrays. These results suggested that RA may affect PTEN activity differently in cell types with more aggressive ovarian cancer cells may not being as susceptible to migration inhibition as the normal cells.
Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 2263.
Footnotes
100th AACR Annual Meeting-- Apr 18-22, 2009; Denver, CO
- American Association for Cancer Research
Volume 69, Issue 9 Supplement
