Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Tumor Biology

Abstract 408: Stem cell marker Nestin regulated the migration and invasion of pancreatic cancer cells

Yoko Matsuda, Toshiyuki Ishiwata, Masao Kawamoto, Tetsushi Yamomoto, Kiyoko Kawahara, Kiyoshi Teduka, Wei-Xia Peng, Kazuya Yamahatsu, Murray Korc and Zenya Naito
Yoko Matsuda
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Toshiyuki Ishiwata
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Masao Kawamoto
2Department of Surgery, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tetsushi Yamomoto
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiyoko Kawahara
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kiyoshi Teduka
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Wei-Xia Peng
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kazuya Yamahatsu
2Department of Surgery, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Murray Korc
3Department of Medicine, Dartmouth Medical School and Dartmouth Hitchcock Medical Center, Lebanon, NH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zenya Naito
1Department of Pathology, Integrative Oncological Pathology, Nippon Medical School, Tokyo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.AM10-408 Published April 2010
  • Article
  • Info & Metrics
Loading
Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC

Abstract

Background: Nestin, a class VI intermediate filament protein, was originally described as a neuronal stem cell/progenitor cell marker. Nestin expression has also been shown to be up-regulated in progenitor cells in muscle, testis, teeth and pancreas. In the pancreas, lineage-tracing experiments have indicated that exocrine cells are derived from Nestin-expressing progenitor cells. Moreover, activation of Kras in the Nestin cell lineage is sufficient for the initiation of premalignant pancreatic intraepithelial neoplasia lesions in mice. We have reported that Nestin was expressed in 30% of human pancreatic ductal adenocarcinoma (PDAC) cases, and Nestin expression in PDAC positively correlated with nerve involvement and invasion of the peripancreatic tissue margin. Therefore, we hypothesized that Nestin may play an important role in the migration and invasive potential of PDAC cells. In this study, we used a silencing strategy to clarify the roles of Nestin in human pancreatic cancer cells. Methods: An expression vector carrying a short hairpin RNA (shRNA) targeting Nestin was stably transfected into PANC-1 and PK-45H human pancreatic cancer cells, which express high levels of Nestin. Changes in the morphology and alignment of actin filaments were analyzed using phase-contrast images and immunocytochemistry. Effects of decreased expression of Nestin on cell growth, migration in scratch and Boyden chamber assays, invasion through matrigel, and cell adhesion on extracellular matrices were examined. Differences in mRNA levels of selected signaling molecules were examined by PCR arrays. Results: Targeting Nestin with shRNA caused a marked decrease in Nestin mRNA and protein levels. Nestin shRNA-transfected cells (Nes-sh cells) exhibited a sheet-like appearance with tight cell-cell adhesion, and increased expression of filamentous (F)-actin by comparison with sham-transfected cells, whereas anchorage-dependent and -independent cell growth and cell-extracellular matrix adhesion of Nes-sh cells did not differ from those of sham cells. By contrast, migration and invasion of Nes-sh cells were markedly attenuated. To clarify the mechanisms underlying this observation, we analyzed differences in selected signaling molecules by PCR arrays that could determine the expression of mRNA closely related to tumor metastasis. The gene expressing the greatest value in Nes-sh cells was E-cadherin. Real-time PCR and western blotting confirmed that E-cadherin expression was increased in Nes-sh cells by comparison with sham cells. Conclusion: Nestin participates in the regulation of pancreatic cancer cell migration and invasion, in part, by altering F-actin and E-cadherin expression. These observations suggest that Nestin may modulate the migration of Nestin-positive progenitor cells during pancreatic development, and may serve as a novel target for suppression of invasion and metastasis in pancreatic cancer.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 408.

  • ©2010 American Association for Cancer Research
Back to top
Cancer Research: 70 (8 Supplement)
April 2010
Volume 70, Issue 8 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract 408: Stem cell marker Nestin regulated the migration and invasion of pancreatic cancer cells
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Abstract 408: Stem cell marker Nestin regulated the migration and invasion of pancreatic cancer cells
Yoko Matsuda, Toshiyuki Ishiwata, Masao Kawamoto, Tetsushi Yamomoto, Kiyoko Kawahara, Kiyoshi Teduka, Wei-Xia Peng, Kazuya Yamahatsu, Murray Korc and Zenya Naito
Cancer Res April 15 2010 (70) (8 Supplement) 408; DOI: 10.1158/1538-7445.AM10-408

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract 408: Stem cell marker Nestin regulated the migration and invasion of pancreatic cancer cells
Yoko Matsuda, Toshiyuki Ishiwata, Masao Kawamoto, Tetsushi Yamomoto, Kiyoko Kawahara, Kiyoshi Teduka, Wei-Xia Peng, Kazuya Yamahatsu, Murray Korc and Zenya Naito
Cancer Res April 15 2010 (70) (8 Supplement) 408; DOI: 10.1158/1538-7445.AM10-408
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Tumor Biology

  • Abstract SY34-04: Do we need to know what pO2 hypoxia is
  • Abstract SY28-04: Rational incorporation of novel agents into multimodality treatment of glioma and neuroblastoma
  • Abstract SY28-02: Connections in the BRCA1-BRCA2 pathway of homologous recombination: Implications for breast cancer development and treatment
Show more 3

Poster Presentations - Proffered Abstracts

  • Abstract LB-236: Risk of tMDS/AML after chemotherapy for first primary lymphoid malignancy, 2000-2013
  • Abstract LB-001: Impact of direct physician engagement with racial/ethnic minorities for oncology clinical trial access and accrual model
  • Abstract LB-107: EV-TRACK: transparent reporting and centralizing knowledge of extracellular vesicles to support the validation of extracellular vesicle biomarkers in cancer research
Show more 3

Poster Presentations - Biomarkers, Determinants of Invasiveness, and Therapeutics That Target Invasion

  • Abstract 434: A novel extracellular chaperokine function of Hsp90 is essential for EphA2-driven cell migration in glioblastoma
  • Abstract 424: Invasion and metastasis of human colon cancer cells is prevented by aldose reductase inhibition
  • Abstract 412: Isolation and analysis of metastasis-related genes in an oral cancer cells with autocrine stromal cell-derived factor −1/CXCR4 system
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement