Skip to main content
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

  • Register
  • Log in
Advertisement

Main menu

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
  • AACR Publications
    • Cancer Discovery
    • Cancer Epidemiology, Biomarkers & Prevention
    • Cancer Immunology Research
    • Cancer Prevention Research
    • Cancer Research
    • Clinical Cancer Research
    • Molecular Cancer Research
    • Molecular Cancer Therapeutics

User menu

  • Register
  • Log in

Search

  • Advanced search
Cancer Research
Cancer Research

Advanced Search

  • Home
  • About
    • The Journal
    • AACR Journals
    • Subscriptions
    • Permissions and Reprints
    • Reviewing
  • Articles
    • OnlineFirst
    • Current Issue
    • Past Issues
    • Focus on Computer Resources
    • 75th Anniversary
    • Meeting Abstracts
  • For Authors
    • Information for Authors
    • Author Services
    • Best of: Author Profiles
    • Submit
  • Alerts
    • Table of Contents
    • OnlineFirst
    • Editors' Picks
    • Citations
    • Author/Keyword
  • News
    • Cancer Discovery News
Tumor Biology

Abstract LB-165: Novel metabolic metyltransferase mediates radiation resistance in glioblastomas

Kamalakannan Palanichamy, Nicolaus Gordon, Disha Patel, Katsuyuki Shirai and Arnab Chakravarti
Kamalakannan Palanichamy
1The Ohio State University Medical Center, Columbus, OH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolaus Gordon
1The Ohio State University Medical Center, Columbus, OH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Disha Patel
1The Ohio State University Medical Center, Columbus, OH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Katsuyuki Shirai
1The Ohio State University Medical Center, Columbus, OH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Arnab Chakravarti
1The Ohio State University Medical Center, Columbus, OH.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
DOI: 10.1158/1538-7445.AM10-LB-165 Published April 2010
  • Article
  • Info & Metrics
Loading
Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC

Abstract

Purpose: Nicotinamide-N-methyltransferase(NNMT) is an enzyme involved in the metabolism of drugs and xenobiotic compounds. It is known to be highly expressed in a variety of cancers and has been investigated for its potential use as a diagnostic or prognostic marker. Furthermore, clinical data has shown that high NNMT expression is correlated with adverse patient outcome. In our glioblastoma (GBM) patient data set and other publically available data set NNMT is one among the top 25 genes which is differentially regulated when compared with normal adjacent tissue and also with various grades of brain tumor. NNMT is highly expressed in grade IV compared to low grade astrocytomas. Kaplan-Meier survival plots for NNMT gene shows that higher expression is associated with poor survival benefit and the median survival was found to be more than doubled in patient group with low expression of NNMT. Despite all this, the mechanistic behavior of NNMT in the malignant phenotype remains uncharacterized. We plan to gain an understanding of the signaling pathways with which NNMT interacts and elucidate its role in radioresistance.

Experimental Design: The in vitro radiosensitivity assays were evaluated using wild type, NNMT-knockdown and NNMT-overexpression GBM cell lines. The assay conducted includes MTS, DNA damage and repair, Clonogenic survival assay, comet assay, Annexin V apoptosis assay, matrigel invasion assays, ATP assay, etc. The cell line after treatment was lysed and probed for pro-and anti-survival signaling pathways.

Results and Discussion: We explored the expression profile of NNMT in established (ATCC) and in our panel of primary GBM cell lines. The expression levels correlates with relative radioresistance exhibited by the cell line. It appears NNMT knockdown cell lines exhibited an enhanced radiosensitivity. Currently we are probing signaling cascades which are differentially regulated in the NNMT knockdown and overexpression cell lines and also the differential response to radiation treatment based on differing levels of NNMT expression. The results will be presented at the meeting.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr LB-165.

  • ©2010 American Association for Cancer Research
Back to top
Cancer Research: 70 (8 Supplement)
April 2010
Volume 70, Issue 8 Supplement
  • Table of Contents
  • Index by Author

Sign up for alerts

Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Cancer Research article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Abstract LB-165: Novel metabolic metyltransferase mediates radiation resistance in glioblastomas
(Your Name) has forwarded a page to you from Cancer Research
(Your Name) thought you would be interested in this article in Cancer Research.
Citation Tools
Abstract LB-165: Novel metabolic metyltransferase mediates radiation resistance in glioblastomas
Kamalakannan Palanichamy, Nicolaus Gordon, Disha Patel, Katsuyuki Shirai and Arnab Chakravarti
Cancer Res April 15 2010 (70) (8 Supplement) LB-165; DOI: 10.1158/1538-7445.AM10-LB-165

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
Share
Abstract LB-165: Novel metabolic metyltransferase mediates radiation resistance in glioblastomas
Kamalakannan Palanichamy, Nicolaus Gordon, Disha Patel, Katsuyuki Shirai and Arnab Chakravarti
Cancer Res April 15 2010 (70) (8 Supplement) LB-165; DOI: 10.1158/1538-7445.AM10-LB-165
del.icio.us logo Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
Advertisement

Related Articles

Cited By...

More in this TOC Section

Tumor Biology

  • Abstract SY34-04: Do we need to know what pO2 hypoxia is
  • Abstract SY28-04: Rational incorporation of novel agents into multimodality treatment of glioma and neuroblastoma
  • Abstract SY28-02: Connections in the BRCA1-BRCA2 pathway of homologous recombination: Implications for breast cancer development and treatment
Show more 3

Poster Presentations - Late-Breaking Abstracts

  • Abstract LB-293: Targeting the PI3K pathway to intercept cetuximab tolerance in metastatic colorectal cancer
  • Abstract LB-304: Discovery of chemical probe CCT251236: An orally bioavailable efficacious pirin ligand from an HSF1 phenotypic screen
  • Abstract LB-302: Potential role of larotrectinib (LOXO-101), a selective pan-TRK inhibitor, in NTRK fusion-positive recurrent glioblastoma
Show more 3

Late-Breaking Poster Presentations - Molecular Modulators of Radiation Response

  • Abstract LB-166: Characterization of a novel class of carbazole based radiation sensitizers
  • Abstract LB-376: Prediction of clinical outcome using NFkB target gene expression based classifier in head and neck patients with different HPV status
Show more 3
  • Home
  • Alerts
  • Feedback
Facebook  Twitter  LinkedIn  YouTube  RSS

Articles

  • Online First
  • Current Issue
  • Past Issues
  • Meeting Abstracts

Info for

  • Authors
  • Subscribers
  • Advertisers
  • Librarians
  • Reviewers

About Cancer Research

  • About the Journal
  • Editorial Board
  • Permissions
  • Submit a Manuscript
AACR logo

Copyright © 2018 by the American Association for Cancer Research.

Cancer Research Online ISSN: 1538-7445
Cancer Research Print ISSN: 0008-5472
Journal of Cancer Research ISSN: 0099-7013
American Journal of Cancer ISSN: 0099-7374

Advertisement