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Emerging Technologies for Cancer Research

Abstract IA17: Microfluidic measurements of single cell mass reveal how growth and division are coordinated

Scott Manalis
Scott Manalis
1Massachusetts Institute of Technology, Cambridge, MA.
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DOI: 10.1158/1538-7445.FBCR11-IA17 Published September 2011
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Abstracts: Second AACR International Conference on Frontiers in Basic Cancer Research--Sep 14-18, 2011; San Francisco, CA

Abstract

Cell growth during the cell cycle must be coordinated with genome replication and segregation, but the mechanisms underlying this coordination are poorly understood, particularly in mammalian cells. My lab is developing suspended microchannel resonators that can precisely measure the size of single cells – mass, volume and density – over time. Using a microfluidic system for culturing single cells over multiple generations while cell mass and cell cycle progression were continuously measured, we monitored over 1,000 hours of single cell growth from mouse lymphoblasts (L1210). I will present recent data from this system and discuss preliminary experiments where the precision cell sizing capabilities of the resonator are used for biomarker analysis.

Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the Second AACR International Conference on Frontiers in Basic Cancer Research; 2011 Sep 14-18; San Francisco, CA. Philadelphia (PA): AACR; Cancer Res 2011;71(18 Suppl):Abstract nr IA17.

  • ©2011 American Association for Cancer Research
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Cancer Research: 71 (18 Supplement)
September 2011
Volume 71, Issue 18 Supplement
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Abstract IA17: Microfluidic measurements of single cell mass reveal how growth and division are coordinated
Scott Manalis
Cancer Res September 15 2011 (71) (18 Supplement) IA17; DOI: 10.1158/1538-7445.FBCR11-IA17

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Abstract IA17: Microfluidic measurements of single cell mass reveal how growth and division are coordinated
Scott Manalis
Cancer Res September 15 2011 (71) (18 Supplement) IA17; DOI: 10.1158/1538-7445.FBCR11-IA17
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Cancer Research Online ISSN: 1538-7445
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